Characterization of pseudobasophilia on Sysmex-XT 1800i automated hematology analyser

Authors

  • Chethana Mannem Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India
  • Thejasvi Krishanmurthy Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India
  • Gayathri Gayathri B. R. Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India
  • Preetha D. Prabhu Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20172594

Keywords:

Automation, Pseudobasophilia, Reactive lymphocytes

Abstract

Background: Pseudobasophilia is a common automation related phenomenon which requires manual peripheral smear study in an era of complete automation. This study has attempted to evaluate the reasons for pseudobasophilia and in-turn suggest measures to eliminate the errors.

Methods: A sample size of 207 cases showing pseudobasophilia on automation were studied by manual peripheral examination to categorize the possible cause for its occurrence. Descriptive and inferential statistical analysis was carried out. Results on continuous measurements are presented on Mean SD and results on categorical measurements are presented in Number (%). Significance is assessed at 5% level of significance. Student t test has been used to find the significance of study parameters on continuous scale within each group.

Results: Atypical/ reactive lymphocytes were present in 86.5% cases contributing to pseudobasophilia phenomenon on automation, which also showed falsely increased absolute basophil count with more percentage of lymphocytes showing reactive changes. Temperature and storage effects did not contribute to their occurrence in this study. Another finding was an associated pseudomonocytosis with pseudobasophilia on automation which was statistically significant (p<0.001).

Conclusions: Pseudobasophilia, and pseudomonocytosis are automation related phenomenon. Atypical/ reactive lymphocytes, which are cytoplasmic strip resistant, contribute to their occurrence. Hence, newer modalities like multicolour flow cytometry coupled with antibody tagging, multiangle polarised scatter separation and volume conductivity scatter may reduce the chances of pseudobasophilia, thereby reducing the overall turnaround time.

Author Biographies

Chethana Mannem, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India

Pathology

Assistant Professor

Thejasvi Krishanmurthy, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India

Pathology

Assistant Professor

Gayathri Gayathri B. R., Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India

Pathology

Tutor

Preetha D. Prabhu, Department of Pathology, Kempegowda Institute of Medical Sciences, Bangalore, Karnataka, India

Pathology

Tutor

References

Jacomo R. What’s the Meaning of Basophilia in Sysmex XE-2100? Arch Pathol Lab Med. 2011;135:415.

Muniraj F, Amritraj V. Pseudobasophilia in Paediatric Age Group. Int J Sci Stud. 2015;3(3):5-10.

Sysmex XT-2000i/XT-1800i Instructions for Use. Sysmex Corporation; Kobe, Japan: 2006.

Amundsen EK, Henriksson CE, Holthe MR, Urdal P. Is the blood basophil count sufficiently precise, accurate, and specific? Three automated hematology instruments and flow cytometry compared. Am J Clin Pathol. 2012;137:86-92.

Ryseff JK. Thesis in partial fulfilment of the requirements for the Degree of Master of Science, Colorado State University, Fort Collins, Colorado. Pseudobasophilia in the dog: its occurrence and predictability of disease using the Advia 120 hematology analyser. 2012;37-51.

Davies S, Bain BJ. Basophil counts on the Technicon H*1 automated counter. Clin Lab Haematol. 1996;18:35-8.

Piva E, Pelloso M, Ciubotaru D, Penello L, Burlina A, Plebani M. The role of automated analyzers in detecting abnormal granulation of leucocytes in lysosomal storage diseases: Maroteaux–Lamy disease. Am J Hematol. 2013;88:527.

Approach to the Microscopic Evaluation of Blood and Bone Marrow. In: Tkachuk DC, Hirschmann JV, eds: Wintrobe’s Atlas of Clinical Hematology. Philadelphia: Williams & Wilkins; 2007:288.

Bain BJ. The peripheral blood smear. In: Goldman L, Schafer AI, eds. Goldman- Cecil Medicine. 25 ed. Philadelphia, Elsevier Saunders; 2016:1056.

Malathesha MK, Ashwini HN. Hematological manifestations in dengue fever- an observational study. J Evol Medi Dent Sci. 2014;3(9):2245-50.

HAEM-R-LP-Sysmex. NHS file, Haematology and Blood Transfusion Directorate of Laboratory Medicine. 4th Ed. 2002:22.

Hill VL, Simpson VZ, Higgins JM, Hu Z, Stevens RA, Metcalf JA, et al. Evaluation of performance of Sysmex-XT 2000i Hematology Analyzer with Whole Bloods Stored at Room Temperature. Lab Med. 2009;40(12):709-18.

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Published

2017-06-24

How to Cite

Mannem, C., Krishanmurthy, T., Gayathri B. R., G., & Prabhu, P. D. (2017). Characterization of pseudobasophilia on Sysmex-XT 1800i automated hematology analyser. International Journal of Research in Medical Sciences, 5(7), 2912–2916. https://doi.org/10.18203/2320-6012.ijrms20172594

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Section

Original Research Articles