Efficacy of combined phenotypic methods for methicillin-resistant Staphylococcus aureus detection and antibiotic susceptibility

Authors

  • Aliya Shah Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India
  • Bushra Peerzada Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India
  • Varsha A. Singh Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India
  • Ritu Garg Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India
  • Muzzafar Zaman Department of Surgery, SKIMS Medical College, Srinagar, Jammu and Kashmir, India
  • Akif Mutahar Shah Department of Anaesthesia, GMC Srinagar, Jammu and Kashmir, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20221177

Keywords:

MRSA, Phenotypic methods, Antibiotic sensitivity, Resistance

Abstract

Background: The main aim of our study is to demonstrate comparative evaluation of oxacillin disc diffusion (ODD), oxacillin screen agar (OSA), CHROM agar (CA) with cefoxitin disc diffusion (CDD) method for the detection of methicillin-resistant Staphylococcus aureus obtained from various clinical samples.

Methods: This prospective study was conducted to detect Methicillin resistance among staphylococcus aureus by four phenotypic methods isolated from various clinical samples received in the Department of microbiology MMIMSR, Mullana.

Results: The data was statistically analyzed, compiled in form of tables, graphs, percentage and test of significance will also be done wherever necessary (using Microsoft Excel, 2008 version) CDD+ODD+OSA+CA proved to be 100% followed by ODD+OSA+CA and CDD+OSA+CA 82.07% and CDD+ODD+OSA 80.1%.

Conclusions: Combined phenotypic methods are better in evaluating and studying MRSA infections in hospitals as compared to tests done in isolation for proper diagnosis and timely treatment of infections.

Author Biographies

Aliya Shah, Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India

SENIOR RESIDENT ,MICROBIOLOGY

Bushra Peerzada, Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India

SENIOR RESIDENT

Varsha A. Singh, Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India

EX-PROFESSOR AND HOD ,MICROBIOLOGY

Ritu Garg, Department Of Microbiology, GMC Srinagar, Jammu and Kashmir, India

PROFESSOR  OF MICROBIOLOGY

Muzzafar Zaman, Department of Surgery, SKIMS Medical College, Srinagar, Jammu and Kashmir, India

ASSOCIATE PROFESSOR ,SURGERY

Akif Mutahar Shah, Department of Anaesthesia, GMC Srinagar, Jammu and Kashmir, India

senior resident

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Published

2022-04-26

How to Cite

Shah, A., Peerzada, B., Singh, V. A., Garg, R., Zaman, M., & Shah, A. M. (2022). Efficacy of combined phenotypic methods for methicillin-resistant Staphylococcus aureus detection and antibiotic susceptibility. International Journal of Research in Medical Sciences, 10(5), 1066–1071. https://doi.org/10.18203/2320-6012.ijrms20221177

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Original Research Articles