Phytochemical, antioxidant, and antimicrobial effects of methanolic leaf extract of Rhazya stricta decne

Ahmed Saif


Background: In this research, we investigated the antioxidant phytochemicals, antimicrobial and antibiofilm properties of methanolic leaf extract from Rhazya stricta.

Methods: The main components of R. stricta were confirmed through HPLC techniques. The antioxidant activity of methanolic leaf extract was examined by DPPD, ABTS, SOD and Glutathione peroxidase assays.

Results: The results showed that the methanolic extract of R. stricta showed better free radical scavenging properties with the IC50 value 74.2 mg/ml by DPPH assay. And the tested extract showed high SOD and peroxidase activities. The HPLC analysis of leaf extract reveal the presence of rutin. Antibacterial results revealed that methanolic leaf extract of R. stricta had higher anti-bacterial activity against multi drug resistant microbes namely, Bacillus subtilis (3.51 mm), Staphylococcus aureus (1.29 mm), Escherichia coli (3.39 mm), Klebsiella pneumoniae (2.03 mm), Pseudomonas aeruginosa (5.73 mm). In addition, the leaf extract showed strong antibiofilm potential against Pseudomonas aeruginosa at 100 µg/ml.

Conclusions: Overall, the methanolic leaf extract of R. stricta showed significant antioxidant capacity and highly inhibit the bacterial pathogens.


Rhazya stricta, Antibacterial, Antioxidant, HPLC, Anti-biofilm activity

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Franklin MJ, Nivens DE, Weadge JT, Howell PL. Biosynthesis of the Pseudomonas aeruginosa Extracellular Polysaccharides, Alginate, Pel, and Psl. Front Microbio. 2011;2:14-9.

Parsek MR, Singh PK. Bacterial Biofilms: An Emerging Link to Disease Pathogenesis. Annu Rev Microbiol. 2003;57(1):677-701.

Mah TFC, O’Toole GA. Mechanisms of biofilm resistance to antimicrobial agents. Trends in Microbiology. 2001;9(1):34-9.

Edeoga HO, Okwu DE, Mbaebie BO. Phytochemical constituents of some Nigerian medicinal plants. Afr J Biotechnol. 2005;4(7):685-8.

Chung KT, Wong TY, Wei CI, Huang YW, Lin Y. Tannins and Human Health: A Review. Crit Rev Food Sci Nutr. 1998;38(6):421-64.

Kadereit JW, Bittrich V, eds. Flowering Plants. Eudicots: Apiales, Gentianales (except Rubiaceae). United States of America: Springer International Publishing; 2018.

Shaltout KH, El-Sheikh MA. Vegetation of the urban habitats in the Nile Delta region, Egypt. Urban Ecosystems. 2002;6(3):205-21.

Obaid AY, Voleti S, Bora RS. Cheminformatics studies to analyze the therapeutic potential of phytochemicals from Rhazya stricta. Chem Central J. 2017;11(1):11.

Bukhari NA, Al-Otaibi RA, Ibhrahim MM. Phytochemical and taxonomic evaluation of Rhazya stricta in Saudi Arabia. Saudi J Biol Sci. 2017; 24(7):1513-21.

Gilani SA, Kikuchi A, Shinwari ZK, Khattak ZI, Watanabe KN. Phytochemical, pharmacological and ethnobotanical studies ofRhazya stricta Decne. Phytother Res. 2007;21(4):301-7.

Sultana N, Khalid A. Phytochemical and enzyme inhibitory studies on indigenous medicinal plant Rhazya stricta. Natural Product Res. 2010;24(4):305-14.

Abdul-Hameed ZH, Alarif WM, Omer AMS, et al. Selective Anti-proliferative Activity of Indole Alkaloids from Rhazya stricta Decne Leaves. LOC. 2019;16(12):941-7.

Aziz AT, Alshehri MA, Alanazi NA. Phytochemical analysis of Rhazya stricta extract and its use in fabrication of silver nanoparticles effective against mosquito vectors and microbial pathogens. Sci Total Environ. 2020;700:134443.

Abdul-Hameed ZH, Alarif WM, Sobhi TR. New cytotoxic indole-type alkaloids obtained from Rhazya stricta leaves. South African J Botany. 2021;137:298-302.

Mahmood R, Kayani WK, Ahmed T. Assessment of antidiabetic potential and phytochemical profiling of Rhazya stricta root extracts. BMC Complement Med Ther. 2020;20(1):293.

Asiry KA, Zaitoun AA. Evaluation of the toxicity of three plant extracts against the Khapra beetle Trogoderma granarium Everts (Coleoptera: Dermestidae) under laboratory conditions. Revista de la Sociedad Entomológica Argentina. 2020;79(1).

Ali BH, Alqarawi AA, Bashir AK, Tanira MO. Antioxidant action of extract of the traditional medicinal plantRhazya stricta Decne. in rats. Phytother Res. 2000;14(6):469-71.

Al-Dabbagh B, Elhaty IA, Al Hrout A, et al. Antioxidant and anticancer activities of Trigonella foenum-graecum, Cassia acutifolia and Rhazya stricta. BMC Complement Altern Med. 2018;18(1): 240.

Al-Zharani N, Abutaha A. Apoptotic Induction and Anti-Migratory Effects of Rhazya Stricta Fruit Extracts on a Human Breast Cancer Cell Line. Molecules. 2019;24(21):3968.

Murugan K, Samidoss CM, Panneerselvam C. Seaweed-synthesized silver nanoparticles: an eco-friendly tool in the fight against Plasmodium falciparum and its vector Anopheles stephensi? Parasitol Res. 2015;114(11):4087-97.

Re R, Pellegrini N, Proteggente A, Pannala A, Yang M, Rice-Evans C. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine. 1999;26(9-10):1231-7.

Paglia DE, Valentine WN. Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase. J lab Clin Med. 1967;70(1): 158-69.

Balasubramanian M, Anbumegala M, Surendran R, Arun M, Shanmugam G. Elite hairy roots of Raphanus sativus (L.) as a source of antioxidants and flavonoids. 3 Biotech. 2018;8(2):128.

Vanaraj S, Keerthana BB, Preethi K. Biosynthesis, Characterization of Silver Nanoparticles Using Quercetin from Clitoria ternatea L to Enhance Toxicity Against Bacterial Biofilm. J Inorg Organomet Polym. 2017;27(5):1412-22.

Stepanović S, Vuković D, Hola V. Quantification of biofilm in microtiter plates: overview of testing conditions and practical recommendations for assessment of biofilm production by staphylococci. Apmis. 2007;115(8):891-9.

Huston AL, Methe B, Deming JW. Purification, Characterization, and Sequencing of an Extracellular Cold-Active Aminopeptidase Produced by Marine Psychrophile Colwellia psychrerythraea Strain 34H. Appl Environ Microbiol. 2004;70(6):3321-8.

Akhgari A, Laakso I, Seppänen-Laakso T. Analysis of Indole alkaloids from rhazya stricta hairy roots by ultra-performance liquid chromatography-mass spectrometry. Molecules. 2015;20(12):22621-34.

Ferguson PJ, Kurowska EM, Freeman DJ, Chambers AF, Koropatnick J. In vivo inhibition of growth of human tumor lines by flavonoid fractions from cranberry extract. Nutr Cancer. 2006;56(1):86-94.

Yuan JF, Zhang ZQ, Fan ZC, Yang JX. Antioxidant effects and cytotoxicity of three purified polysaccharides from Ligusticum chuanxiong Hort. Carbohydrate Polymers. 2008;74(4):822-7.

Razali N, Razab R, Junit SM, Aziz AA. Radical scavenging and reducing properties of extracts of cashew shoots (Anacardium occidentale). Food Chemistry. 2008;111(1):38-44.

Al-Busafi S, Al-Riyami M, Al-Ouwaisi K, Hisham A. Screening of Antioxidant and Radical Scavenging Activities of Some Omani Medicinal Plants. SQU J Sci. 2007;12(1):1.

Reszka KJ, Britigan BE. Doxorubicin inhibits oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) by a lactoperoxidase/H2O2 system by reacting with ABTS-derived radical. Arch Biochem Biophysics. 2007;466(2):164-71.

Shahinuzzaman M, Yaakob Z, Anuar FH. In vitro antioxidant activity of Ficus carica L. latex from 18 different cultivars. Sci Rep. 2020;10(1):10852.

Kumar U, Mishra M, Prakash V. Assessment of antioxidant enzymes and free radical scavenging activity of selected medicinal plants. Free Radicals Antioxidants. 2012;2(3):58-63.

Sarker U, Oba S. Catalase, superoxide dismutase and ascorbate-glutathione cycle enzymes confer drought tolerance of Amaranthus tricolor. Sci Rep. 2018; 8(1):16496.

Shegute T, Wasihun Y. Antibacterial Activity and Phytochemical Components of Leaf Extracts of Agave americana. Journal of Experimental Pharmacology. 2020;12:447-4.

Chuah EL, Zakaria Z, Suhaili Z, Bakar SA, Desa MNM. Antimicrobial activities of plant extracts against methicillin-susceptible and methicillin-resistant Staphylococcus aureus. J Microbiol Res. 2014;4:6-10.

El-Bashiti TA, Masad AA, Mosleh FN, Madi YMA. Antibacterial and Anti-Biofilm Activity of selected plant extracts against some human pathogenic microorganisms. Pakistan J of Nutr. 2019;18(11): 1014-20.

Shams ‎ S, Mehdipour ‎ A, Kermani ‎ S, Ghorbani ‎ H, Ragolia ‎ S. Anti-Biofilm Activity of punica granatum, ricinus communis, and ‎allium sativum plant extracts on Streptococcus mutans. IEM. 2018;4(2):67-72.

Gomes F, Martins N, Barros L. Plant phenolic extracts as an effective strategy to control Staphylococcus aureus , the dairy industry pathogen. Industrial Crops Products. 2018;112:515-20.