Biofilm producing multidrug resistant Acinetobacter species from a tertiary care hospital: a therapeutic challenge
Keywords:Acinetobacter baumanii, Biofilm, Multidrug resistant
Background: Acinetobacter species are responsible for nosocomial infections which are related to the biofilm forming capacity of this pathogen. Biofilm formation helps the bacteria in surviving stressed environmental conditions and bacteria growing in biofilms are multidrug resistant. The objective was to detect biofilm formation and its relation to drug resistance among Acinetobacter spp isolates.
Methods: A total of 75 clinical isolates of Acinetobacter species were tested for biofilm production by microtitre plate method and susceptibility to antibiotic discs were determined by Kirby Bayer Method.
Results: On testing by tissue culture plate method, 12 (16%) Acinetobacter isolates were weak biofilm producers, 9 (12%) were moderate producers, 30 (40%) were strong biofilm producers, 24 (32%) isolates were nonbiofilm producers and 63 % isolates were multidrug resistant.
Conclusions: This study demonstrates the ability of Acinetobacter isolates to form biofilm and biofilm production has strong association with multiple drug resistance.
Ali AA, Hendiani S, Mohammadi P, Gharavi S. Assessment of biofilm formation and resistance to imipenem and ciprofloxacin among clinical isolates of Acinetobacter baumannii in Tehran. Jundishapur J Microbiol. 2014;7(1):8606.
Karakoc C, Tekin R, Yes IZ, Cagatay A. Risk factors for mortality in patients with nosocomial Gram-negative rod bacteremia. Eur Rev Med. Pharmacol Sci.2013;17:951-7.
Gurung J, Khyriem AB, Banik A, Lyngdoh WV, Choudhury B, Bhattacharyya P. Association of biofilm production with multidrug resistance among clinical isolates of Acinetobacter baumannii and Pseudomonas aeruginosa from intensive care unit. Indian J Crit Care Med. 2013;17:214-8.
Gerner SP, Tjernberg I, Ursing J. Reliability of phenotypic tests for identification of Acinetobacter species. J Clin Microbiol. 1991;29:277-82.
Kenchappa P, Sreenivasmurthy B. Simplified panel of assimilation tests for identification of Acinetobacter species. Indian J Pathol Microbiol. 2003;46:700-6.
Christensen GD, Simpson WA, Younger JA, Baddour LM, Barrett FF, Melton DM, et al. Adherence of coagulase negative Staphylococci to plastic tissue cultures: a quantitative model for the adherence of staphylococci to medical devices. J Clin Microbiol. 1995;22:996-1006.
Clinical and laboratory standards institute. Performance standards for antimicrobial susceptibility testing, 16th informational supplement, M100-S16. Wayne, PA: CLSI, 2006.
Tomaras AP, Dorsey CW, Edelmann RE, Actis LA. Attachment to and biofilm formation on abiotic surfaces by Acinetobacter baumannii: involvement of a novel chaperone-usher pili assembly system. Microbiology. 2003;149:3473-84.
Lee HW, Koh YM, Kim J, Lee JC, Lee YC, Seol SY, et al. Capacity of multidrug-resistant clinical isolates of Acinetobacter baumannii to form biofilm and adhere to epithelial cell surfaces. Clin Microbiol Infect. 2008;14:49-54.
Rao SR, R Uma K, Singh SP, Shashikala P, Kanungo R, Jayachandran S, Prashanth K. Correlation between biofilm production and multiple drug resistance in imipenem resistant clinical isolates of Acinetobacter baumannii. Indian J Med Microbio. 2008;26(4):333-7.
Rodroguez BJ, Marti S, Soto S, Fernandez CF, Cisneros JM, Pachon J, et al. Bio film formation in Acinetobacter baumannii: associated features and clinical implications. Clin Microbiol Infect. 2008;14:276-8.
Dheepa M, Vinitha L, Appalaraju B. Comparision of biofilm production and multiple drug resistance in clinical isolates of Acinetobacter baumannii from a tertiary care hospital in South India. Int J Pharm Biomed Sci. 2011;2(4):103-7.
Vijaya BS, Govindan R, Preeti P, Kurt S, Daniel T, Prapas P, et al. Genetic relatedness and molecular characterization of multidrug resistant Acinetobacter baumannii isolated in central Ohio, USA. Ann Clin Microbial Antimicrob. 2009;8:21.
Beck SCM, Jarvis WR, Brook JH, Culver DH, Potts A, Gay E, et al. Epidemic bacteremia due to Acinetobacter baumannii in five intensive care units. Am J Epidemiol. 1990;132(4):723-33.
Vidal R, Dominguez M, Urrutia H, Bello H, Garcia A, Gonzalez G, et al. Effect of imipenem and sulbactam on sessile cells of Acineto-bacter baumannii growing in biofilm. Microbios. 1997;91(367):79-87.