Fine needle aspiration cytology of salivary gland lesions: study in a tertiary care hospital of North Bihar

Sonal Verma


Background: Fine needle aspiration cytology (FNAC) of suspected salivary gland lesions has an established role in preoperative diagnosis and management of patients. The aim of the present study was to evaluate the spectrum of salivary gland lesions in our setting and to assess the diagnostic accuracy of FNAC for salivary gland lesions.

Methods: In the present study, 189 cases were included in this study and cytohistological correlation was made in 84 cases only. FNA was performed from different sites of the salivary gland swelling using a 10 mL disposable syringe and 23/24-gauge needle without local anaesthesia. FNA air-dried smears were stained with Giemsa stain and wet smears fixed in 95% ethyl alcohol were stained with haematoxylin and eosin stain. Paraffin embedded tissue sections were stained with haematoxylin and eosin (H & E).

Results: Overall diagnostic accuracy of FNAC in our study was found to be 94.05% with 88% sensitivity and 96.6% specificity.

Conclusions: The high accuracy, sensitivity, and specificity of FNAC confirm that preoperative cytology is a useful, quick and reliable diagnostic technique indispensable for developing countries.


FNA salivary gland, Diagnostic accuracy

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Kotwal M, Gaikwad S, Patil R, Munshi M. Fnac of salivary gland- a useful tool in preoperative diagnosis or a cytopathologist’sriddle. Journal of cytology. 2007;24(2):85-8.

Diaz KP, Gerhard R, Domingues RB, Martins LL, Prado Ribeiro AC, Lopes MA. High diagnistic accuracy and reproducibility of fine needle aspiration cytology for diagnosing salivary gland tumors : cytohistologic correlation in 182 cases. Oral Surg Oral Med Oral Pathol Oral Radiol. 2014;118(2):226-35.

Huq AH, Aktaruzzaman M, Habib MA, Islam MS, Amin S. A comparative study between fine needle aspiration cytology findings and histopathological report of major salivary gland neoplasm in a tertiary hospital of bangladesh. Bangladesh Med Res Counc Bull. 2013;39(2):69-73.

Kechagias N, Ntomouchtsis A, Valeri R, Patrikidou A, Kitikidou K, Xirou P. Fine needle aspiration cytology of salivary gland tumors: a 10 years retrospective analysis.”. Oral Maxilofac Surg. 2012;16(1):35-40.

Singh NKD, Mehta A, Nanda J. Fine needle aspiration cytology: a reliable tool in the diagnosis of salivary gland lesions. J Oral Pathol Med. 2012;41(1):106-12.

Qizilbash AH, Sianos J, Young JEM, Archibald SD. Fine needle aspiration biopsy cytology of major salivary glands. Acta Cytologica. 1985;29(4):503-12.

Nguansangiam S, Jesdapatarakul S, Dhanarak N, Sosrisakorn K. Accuracy of fine needle aspiration cytology of salivary gland lesions: routine diagnostic experience in Bangkok, Thailand. Asian Pacific Journal of Cancer Prevention. 2012;13(4):1583-8.

Tan LG, Khoo ML. Accuracy of fine needle aspiration cytology and frozen section histopathology for lesions of the major salivary glands. Annals of the Academy of Medicine Singapore. 2006;35(4):242-8.

Mihashi H, Kawahara A, Kage M. Comparison of preoperative fine-needle aspiration cytology diagnosis and histopathological diagnosis of salivary gland tumors. Kurume Medical Journal. 2006;53(1-2):23-27.

Jan IS, Chung P, Weng M. Analysis of fine-needle aspiration cytology of the salivary gland. Journal of the Formosan Medical Association. 2008;107(5):364-70.

Cajulis RS, Gokaslan ST, Yu GH, Frias-Hidvegi D. Fine needle aspiration biopsy of the salivary glands: a fiveyear experience with emphasis on diagnostic pitfalls. Acta Cytologica. 1997; 41(5):1412-20.

Boccato P, Altavilla G, Blandamura S. Fine needle aspiration biopsy of salivary gland lesions: a reappraisal of pitfalls and problems. Acta Cytologica. 1998;42(4):888-98.

Das DK, Petkar MA, Al-Mane NM, Sheikh ZA, Mallik MK, Anim JT. Role of fine needle aspiration cytology in the diagnosis of swellings in the salivary gland regions: a study of 712 cases. Medical Principles and Practice. 2004;13(2):95-106.

Choudhury AA, Sultana T, Siddique BH, Amin ASA. Diagnosis of parotid gland mass by the fine needle aspiration cytology (FNAC) and its histopathological correlation-2 years study in BSMMU, Dhaka. Bangabandhu Sheikh Mujib Medical University Journal. 2011;4(2):65-9.

Murai N, Taniguchi Z, Takahashi Y, Kuboshima F, Tateya I. A study of salivary gland aspiration cytology reporting: guideline validity. Nihon Jibiinkoka Gakkai Kaiho. 2011;114(7):615-9.

Piccioni LO, Fabiano B, Gemma M, Sarandria D, Bussi M. Fine needle aspiration cytology in the diagnosis of parotid lesions. Acta Otorhinolaryngol Ital. 2011;31(1):1-4.

Singh A, Haritwal A, Murali B. Correlation between cytology and histopathology of the salivary gland. Australas Med J. 2011;4(2):66-71.

Young JA. Diagnostic problems in fine needle aspiration cytopathology of the salivary glands. J Clin Pathol. 1994;47:193-8.

Klijanienko J, Vielh P, Batsakis JD. Monographs in clinical cytology. Vol. 15. Salivary gland tumours. Basel, Switzerland: Karger. 2000.

Jain R, Gupta R, Kudesia M, Sing S. Fine needle aspiration cytology in the diagnosis of salivary gland lesions: a study with histologic comparison. Cytojournal. 2013;10:5.

Orell SR, Sterrett GF, Whitaker D. Fine Needle Aspiration Cytology. 4/e. 2005;17(4):41-82.

Omhare A, Singh SK, Nigam JS, Sharma A. Cytohistopathological study of salivary gland lesions in Bundelkhand region, Uttar Pradesh, India. Pathology Research International. 2014.