Microorganisms causing wound infection and their antibiotic susceptibility pattern
DOI:
https://doi.org/10.18203/2320-6012.ijrms20222869Keywords:
Antibiogram, Bacterial isolates, Drug resistance, MRSA, PusAbstract
Background: Pyogenic infection is one of major causes of morbidity and increasing medical expense. Multiple organisms can cause wound infection. Drug resistant bacteria are the most important therapeutic challenge.
Methods: A prospective study was carried out from July 2021 to October 2021and pus samples were collected from suspected OPD and indoor patients with wound infection. The pus specimen after appropriate aerobic culture, the bacteria grown was identified by colony morphology, staining reaction and different standard biochemical tests. Modified Kirby Bauer disc diffusion method was used for the antimicrobial susceptibility testing.
Results: Out of 140 pus samples from wound infection of suspected patients, 88 (62.9%) showed bacterial growth and most of them were from male patients, 66 (64.70%). Most of the isolates were isolated from in-patient (52, 59.1%). Staphylococcus aureus was the most prevalent pathogen (38.6%) followed by Klebsiella spp. (22.7%). Most of the S. aureus were MRSA (32.4%) and sensitive to vancomycin, linezolid and teicoplanin. Klebsiella spp. and other Gram-negative bacteria isolates were mostly sensitive to imipenem and they were highly resistant to co-trimoxazole, cephalosporins.
Conclusions: The organisms mainly attributing for pyogenic wound infections are S. aureus, Klebsiella species. High level of drug resistance was seen for both Gram positive and Gram-negative bacteria. Continuous surveillance is necessary. As the microorganisms isolated were mostly resistant to different antibiotic classes, so effective surveillance and proper implementation of local antibiotic policy is needed.
Metrics
References
Church D, Elsayed S, Reid O, Winston B, Lindsay R. Burn wound infection. Clin Microbiol Rev. 2006;19:403-34.
Gupta N, Gautam V, Saini S, Singh L, Arora DR. Prevalence of multi drug organism in wound infection. J infect Dis Antimicrob Agent. 2002;19:111-7.
Esebelahie NO, Esebelahie FO, Omoregie R. Aerobic bacterial isolates from wound infection. Afr J Cln Exper Microbial. 2013;14(3):155-9.
Nichole RE. Preventing surgical site infection: a surgeon’s perspective. Emerg Infect Dis. 2001;7(2):220-4.
Atiyeh BS, Costagliola M, Hayek SN, Dibo SA. Effect of silver on burn wound infection control and healing: review of the literature. Burns. 2007;33:2:139-48.
Cutting KF, White R. Defined and refined: criteria for identifying wound infection revisited. Br J Community Nurs. 2004;9(3):6-15.
Dionigi R, Rovera F, Dionigi G, Imperatori A, Ferrari A, Dionigi P, et al. Risk factor in surgery. J Chemother. 2001;13:6-11.
Bowler PG, Duerden BI, Amstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev. 2001;14:244-69.
Mahat P, Manandhar S, Baidya B. Bacteriological profile of wound infection and antibiotic susceptibility pattern of the isolates. J Microbiol Exp. 2017;4(5):119-22.
Kc R, Shrestha A, Sharma VK. Bacteriological study of wound infection and antibiotic susceptibility pattern of the isolates. Nepal J Sci Tech. 2013;14(2):143-50.
Rijal BP, Satyal D, Parajuli NP. High burden of antimicrobial resistance among bacteria causing pyogenic wound infections at a tertiary care hospital in Kathmandu, Nepal. J Pathogens. 2017;P1-7.
Rai S, Yadav UN, Pant ND, Yakha JK, Tripathi PP, Poudel A, et al. Bacteriological profile and antimicrobial susceptibility patterns of bacteria isolated from pus/wound swab samples from children attending a tertiary care hospital in Kathmandu, Nepal. Int J Microbiol. 2017;2017.
Mantravadi HB, Chinthaparthi MR, Shravani V. Aerobic isolates in pus and their antibiotic sensitivity pattern: a study conducted in a teaching hospital in Andhra Pradesh. Int J Med Sci Public Health. 2015;4:1076-9.
Trojan R, Razdan L, Singh N. Antibiotic susceptibility patterns of bacterial isolates from pus samples in a tertiary care hospital of Punjab, India. Int J Microbiol. 2016;2016.
Sangwan J, Singla P, Mane P, Lathwal S, Malik AK. Prevalence and antimicrobial susceptibility patterns of aerobic bacterial isolates from pyogenic wound infections at a tertiary care institute in Haryana, India. Int J Curr Microbiol App Sci. 2016;5(2):78-85.
Kalita JM, Nag VL, Kombade S, Yedale K. Multidrug resistant superbugs in pyogenic infections: a study from Western Rajasthan, India. Pan Afr Med J. 2021;38:409.
Taneja S, Singh G. Study of most common bacterial isolates causing pyogenic infections. Eur J Mol Clin Med. 2020;07(07):4029-41.
Downloads
Published
How to Cite
Issue
Section
