Antimicrobial susceptibility profile of aerobic bacterial strains isolated from periodontal lesions
DOI:
https://doi.org/10.18203/2320-6012.ijrms20222834Keywords:
Periodontitis, Aerobic culture, Drug resistanceAbstract
Background: Periodontitis or gum disease is a serious gum disease that damages soft tissue and without treatment can destroy the bone that support the teeth. It is because of consequences of specific or non-specific bacterial infections. Drug resistance is a major concern in treatment of periodontitis also. So this study was planned to isolate, identify aerobic bacteria and to characterize the antibiotic sensitivity pattern of isolate from periodontal lesions and to analyze host/patient related factors.
Methods: Patients coming to dental OPD with signs and symptoms of periodontitis were selected for the study. Samples were collected from periodontal pockets using bent swab stick and were processed for aerobic culture and antibiotic susceptibility test. Demographic data, results of culture, isolated pathogens and its antimicrobial sensitivity related data were entered in Excel sheet and analyzed using SPSS software version 23.
Results: Total 71 aerobic isolates were found out of 100 aerobic culture. In E.coli isolates 83% resistance was found for ceftriaxone and ceftazidime. K.oxytoca isolates show 75% resistance to ceftazidime. K.pneumonaie shows 75% resistance to ceftazidime, 50% to amoxiclav, 29% to minocyclin. S.viridans shows 22% resistance to azithromycin, 19% to tetracycline and erythromycin. In present study higher drug resistance was found in K.pneumonaie followed by E.coli, K.oxytoca, and S.viridans. There is significant association between dental hygiene and the culture result (chi-square=20.771, df=8. p<0.01) and no significant association between age and the culture result (chi-square=44.032, df=40, p=0.305) were found.
Conclusions: Periodontitis patients shows drug resistant to commonly used antibiotics for the same. Also, there is an association between dental hygiene and culture positivity. So proper dental hygiene can prevent periodontitis.
References
Craig RG, Yip JK, So MK, Boylan RJ, Socransky SS, Haffajee AD. Relationship of destruct- tive periodontal disease to the acute-phase. J Periodontol. 2003;74(7):1007-16.
Antipa C, Dascalu L, Chifiriuc MC, Lazar V, Bleotu CC, Ruta SM. Isolation, identification and antibiotic susceptibility profiles in bacterial strains isolated from periodontal lesions. Ann Biol Res 2014;5(3):22-6.
Galante DL. History and current use of clinical photography in orthodontics. J Calif Dent Assoc. 2009;37(3):173-4.
Pöllänen MT, Laine MA, Ihalin et al. Host-bacteria crosstalk at the dentogingival junction. Int J Dent. 2012:821383.
Kuramitsu HK, He X, Lux R, Anderson MH, Shi W. Interspecies interactions within oral microbial communities. Microbiol Mol Biol Rev 2007;71(4):653-70.
Bartold PM, Van Dyke TE. Periodontitis: a host-mediated disruption of microbial homeostasis. Unlearning learned concepts. Periodontol 2000. 2013;62(1):203-17.
Socransky SS, Haffajee AD, Cugini MA, Smith C, Kent RL. Microbial complexes in subgingival plaque. J Clin Periodontol. 1998;25(2):134-44.
Darveau RP. Periodontitis: a polymicrobial disruption of host homeostasis. Nat Rev Microbiol. 2010;8(7):481-90.
Kassebaum NJ, Bernabé E, Dahiya M, Bhandari B, Murray CJ, Marcenes W. Global burden of severe periodontitis in 1990–2010: A systematic review and meta-regression. J Dent Res. 2014;93:1045–53.
Genco RJ. Borgnakke, W.S. Risk factors for periodontal disease. Periodontol. 2013;6259–94.
Sakthivel M, Ayyasamy PM, Arvind Prasanth D. Identification and antimicrobial susceptibility testing of pathogenic micro-organism from dental patients. Asian J Pharm Clin Res. 2016;9(2):226-30.
Cristiana A, Luminița D, Mariana CC, Veronica L, Coralia B, Simona MR. Isolation, identification and antibiotic susceptibility profiles in bacterial strains isolated from periodontal lesions. Ann Biological Res. 2014;5(3):22-6.
