Evaluation of mucin histochemistry in relation to p63 expression in nodular hyperplasia and adenocarcinoma of prostate


  • Rumana Afrin Sweety Department of Pathology, Marks Medical College, Dhaka, Bangladesh
  • Shifayat Sultana Department of Pathology, Central Medical College, Cumilla, Bangladesh
  • M. Ariful Islam Department of Pathology, Monowara Sikder Medical College, Shariatpur, Bangladesh
  • M. Rezaul Karim Dewan Department of Pathology, Green Life Medical College, Dhaka, Bangladesh
  • Mahbuba Zhumur Department of Pathology, Dhaka Medical College, Dhaka, Bangladesh
  • Ruksana Jeba Department of Pathology, Dhaka Medical College, Dhaka, Bangladesh
  • Nazma Shaheen Department of Pathology, Sheikh Fazilatunnessa Mujib Memorial KPJ Specialized Hospital and Nursing College, Gazipur, Bangladesh




NHP, Prostatic adenocarcinoma, Mucin histochemistry, p63


Background: Prostate cancer is a leading cause of death in men. Nodular hyperplasia and adenocarcinoma are common causes of prostatic enlargement. Diagnosis of these lesions on routine biopsies can be difficult for pathologists. Immunohistochemical stain p63 can help, but it is costly and not widely available. The present study aimed to evaluate the usefulness of mucin histochemistry in relation to p63 expression in differentiating nodular hyperplasia and adenocarcinoma of prostate.

Methods: This study was conducted in the department of pathology at Dhaka medical college from July 2018 to June 2020. 50 cases of prostatic lesions (25 NHP and 25 adenocarcinoma) were examined using histomorphology. The sections were stained with periodic acid Schiff (PAS) to identify neutral mucins and Alcian blue (2.5 pH) to identify acidic mucins. Additionally, p63 antibody was used in immunohistochemistry.

Results: NHP showed positivity for neutral mucin (96% with PAS stain) but not for acidic mucin (Alcian blue stain), while prostatic carcinoma showed positivity for both neutral mucin (28%) and acidic mucin (44%). The grade group 1 tumors of prostatic carcinoma showed 100% positivity for acid mucin, with a decrease in Alcian blue staining as the grade increased. P63 was positive in 100% of NHP cases and negative in 100% of prostatic carcinoma cases.

Conclusions: Positivity for acidic mucins with Alcian blue stain can be a helpful diagnostic tool to differentiate well differentiated adenocarcinomas from benign lesions where facility for p63 immuno-stain is not available and poor people who cannot afford the cost of immunohistochemistry.


Ambali MP, Doshi MA, Ganga GM, Kanetkar SV, Kakade SV. Study of Mucin Histochemistry in Benign hyperplasia and Malignant Lesions of Human Prostate. Pravara Med Rew. 2018;10:3.

Berry SJ, Coffey DS, Walsh PC, Ewing LL. The development of human benign prostatic hyperplasia with age. J Urol. 1984;132(3):474-9.

Ferlay J, Colombet M, Soerjomataram I, Mathers C, Parkin DM, Piñeros M, Znaor A, Bray F. Estimating the global cancer incidence and mortality in 2018: GLOBOCAN sources and methods. Int J Cancer. 2019;144(8):1941-53.

Rawla P. Epidemiology of prostate cancer. World J Oncol. 2019;10(2):63.89.

Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A. Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA Cancer J Clinicians. 2018;68(6):394-424.

Humphery PA. Diagnosis of adenocarcinoma in prostate needle biopsy tissue. J Clin Pathol. 2007;6035-42.

Srigley JR. Benign mimickers of prostatic adenocarcinoma. Modern Pathol. 2004;17(3):328-48.

Signoretti S, Waltregny D, Dilks J, Isaac B, Lin D, Garraway L et al. p63 is a prostate basal cell marker and is required for prostate development. Am J Pathol. 2000;157(6):1769-75.

Rasheed IA, Hussein AG. Basal cell markers:34BE12 and p63, improving detection of basal cells in atypical prostatic lesions’ Al-Kindy College Med J. 2017;13:60-3

Totten RS, Heinemann NW, Hudson PB, Sproul EE, Stout AP. Microscopic differential diagnosis of latent carcinoma of the prostate. Arch Pathol Lab Med. 1953;55:131-41.

Agrawal DN, Zawar MP, Deshpande NM, Sudhamani S. The study of mucin histochemistry in benign and malignant lesions of prostate. J Scientific Society. 2014;41(1):38.

Bastola S, Talwar OP. Evaluation of mucin histochemistry in benign and malignant prostatic lesion and their correlation PSA level. J Pathol Nepal. 2014;4:612.

Kiruthika N. A study on mucin histochemistry and p63 expression in benign and malignant prostatic lesions. Available at: repository-tnmgrmu.ac.in. 2019. Accessed on 17 November 2022.

Khanna A, Patil R, Deshmukh A. Assessment of the Potential of Pathological Stains in Human Prostate Cancer. J Clin Diagnostic Res. 2014;8:124-8.

Nisar B, Sarwar N, Sharif S, Hameed A, Naz S. Expression of p63 Protein to Differentiate Benign Prostatic Hyperplasia and Carcinoma of Prostate in Pakistani Population. ResearchGate. 2017;23:196-201.

Kalantari MR, Anvari K, Jabbari H, Tabrizi FV. p63 is more sensitive and specific than 34βE12 to differentiate adenocarcinoma of prostate from cancer mimickers. Iran J Med Sci. 2014;17:497-501.




How to Cite

Sweety, R. A., Sultana, S., Islam, M. A., Dewan, M. R. K., Zhumur, M., Jeba, R., & Shaheen, N. (2023). Evaluation of mucin histochemistry in relation to p63 expression in nodular hyperplasia and adenocarcinoma of prostate. International Journal of Research in Medical Sciences, 11(3), 824–828. https://doi.org/10.18203/2320-6012.ijrms20230302



Original Research Articles