High performance thin layer chromatography analysis of seed extracts of Tudri Surkh (Cheiranthus cheiri)
DOI:
https://doi.org/10.18203/2320-6012.ijrms20223626Keywords:
Unani, Tudri Surkh, Cheiranthus cheiri, HPTLC, StandardizationAbstract
Background: To develop the high-performance thin layer chromatography (HPTLC) finger print profile of hydroalcoholic and ethylalcohol extracts of seeds of Tudri Surkh (Cheiranthus cheiri).
Methods: Chromatographic technique was used for separation of components from hydroalcoholic and ethylalcohol extracts of seeds and HPTLC was carried out using CAMAG HPTLC system equipped with Linomat V applicator, and WinCATS software.
Results: HPTLC profiling of the extract confirm the presence of various phytochemicals. At 640 nm HPTLC finger print of hydroalcoholic extract revealed 16 components with Rf=0.05-0.94 while ethylalcohol extracts revealed 19 components peaks with Rf=0.07 to 0.96. Well separated and compact greenish, yellow and pink bands were visualized using anisaldehyde sulphuric acid reagent.
Conclusions: We conclude that HPTLC fingerprint profiling of seed extracts of Tudri Surkh (Cheiranthus cheiri) revels presence of various compounds and can be utilized as a marker for standardization and proper identification of the plant material to be used for preparation of traditional drugs.
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- 2023-01-03 (2)
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