Protective effects of pomiferin isolated from Maclura pomifera on ischemia-reperfusion injury of rat ovary: biochemical and histopathologic evaluation
DOI:
https://doi.org/10.18203/2320-6012.ijrms20230010Keywords:
Oxidative stress, Pomiferin, Ischemia/reperfusion injury, Steril inflammationAbstract
Background: The aim of this study was to investigate the antioxidant and anti-inflammatory properties of pomiferin, a prenylated flavonoid was purified from Maclura pomifera by thin layer chromatography method, on oxidative stress, sterile inflamation and ovarian tissue damage caused by ischemia-reperfusion model.
Methods: Thirty female Wistar albino rats were divided into five groups. In the group CN only laparotomy operation was performed. In group CNPomiferin, rats received 200 mg/kg pomiferin. In group IRVehicle, reperfusion for 3 h performed after an ischemic period of 3 hours. In groups IRPomiferin100 and IRPomiferin200 rats received 100 mg/kg and 200 mg/kg doses of pomiferin, by oral gavage 1 houes before reperfusion. After the experiments, tissue level of malondialdehyde (MDA) and activities of myeloperoxidase (MPO), catalase (CAT), superoxide dismutase (SOD) were determined, and histopathological changes were examined in all rat ovarian tissue.
Results: It was determined that irreversible cell damage such as apoptotic and necrotic deaths and reversible cell damage occurred in follicular, endothelial and stromal cells due to oxidative stress in the group IRVehicle. In this group, it was determined that the LPO level exceeded the cellular antioxidant capacity (SOD and CAT enzyme activities) and the PMNL infiltration and activity (MPO), an indicator of sterile inflammation, increased. It was determined that oxidative stress sterile inflammation and irreversible cell damage decreased in a dose-dependent manner with pomiferin treatment.
Conclusions: Pomipherin treatment strongly protects ovarian follicles and vascular structures against ischemia-reperfusion injury, thus it may prevent the reduction of ovarian follicle reserve, which is an indicator of female fertility.
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References
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Cigsar G, KELEŞ O, Can S, et al. The protective effects of osajin on ischemia/reperfusion injury to rat ovaries: biochemical and histopathological evaluation. Kafkas Universitesi Veteriner Fakultesi Dergisi 2015;21;5:
Hosek J, Toniolo A, Neuwirth O, Bolego C. Prenylated and geranylated flavonoids increase production of reactive oxygen species in mouse macrophages but inhibit the inflammatory response. Journal of natural products 2013;76;9:1586-91.
Yang R, Hanwell H, Zhang J, Tsao R, Meckling KA. Antiproliferative activity of pomiferin in normal (MCF-10A) and transformed (MCF-7) breast epithelial cells. Journal of agricultural and food chemistry 2011;59;24:13328-36.
Vesela D, Kubinova R, Muselik J, Zemlicka M, Suchy V. Antioxidative and EROD activities of osajin and pomiferin. Fitoterapia 2004;75;2:209-11.
Tsao R, Yang R, Young JC. Antioxidant isoflavones in Osage orange, Maclura pomifera (Raf.) Schneid. Journal of agricultural and food chemistry 2003;51;22:6445-51.
Diopan V, Babula P, Shestivska V, et al. Electrochemical and spectrometric study of antioxidant activity of pomiferin, isopomiferin, osajin and catalposide. Journal of pharmaceutical and biomedical analysis 2008;48;1:127-33.
Bartosikova L, Necas J, Bartosik T, Pavlik M, Frana P. Effect of pomiferin administration on kidney ischaemia-reperfusion injury in rats. Interdisciplinary toxicology 2010;3;2:76-81.
Florian T, Necas J, Bartosikova L, et al. Effects of prenylated isoflavones osajin and pomiferin in premedication on heart ischemia-reperfusion. Biomedical papers of the Medical Faculty of the University Palacky, Olomouc, Czechoslovakia 2006;150;1:93-100.
Abourashed EA, Abraha A, Khan SI, McCants T, Awan S. Potential of Horse Apple Isoflavones in Targeting Inflammation and Tau Protein Fibrillization. Natural product communications 2015;10;9:1577-80.
Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Analytical biochemistry 1979;95;2:351-358.
Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical chemistry 1988;34;3:497-500.
Aebi H. Catalase in vitro. Methods in enzymology. Elsevier; 1984:121-126.
Bradley PP, Priebat DA, Christensen RD, Rothstein G. Measurement of cutaneous inflammation: estimation of neutrophil content with an enzyme marker. Journal of investigative dermatology 1982;78;3:206-209.
Eltzschig HK, Eckle T. Ischemia and reperfusion--from mechanism to translation. Nature medicine 2011;17;11:1391-401.
Sen S, Chakraborty R. The role of antioxidants in human health. Oxidative stress: diagnostics, prevention, and therapy. ACS Publications; 2011:1-37.
Ouyang L, Shi Z, Zhao S, et al. Programmed cell death pathways in cancer: a review of apoptosis, autophagy and programmed necrosis. Cell proliferation 2012;45;6:487-98.
Yoo MH, Gu X, Xu XM, et al. Delineating the role of glutathione peroxidase 4 in protecting cells against lipid hydroperoxide damage and in Alzheimer's disease. Antioxidants & redox signaling 2010;12;7:819-27.
Oztay F, Kara-Kisla B, Orhan N, Yanardag R, Bolkent S. The protective effects of prostaglandin E1 on lung injury following renal ischemia-reperfusion in rats. Toxicology and industrial health 2016;32;9:1684-92.
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Cigremis Y, Kart A, Karaman M, Erdag D. Attenuation of ischemia-reperfusion injury with Marrubium cordatum treatment in ovarian torsion-detorsion model in rabbits. Fertility and sterility 2010;93;5:1455-63.
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Bhattacharya S. Reactive oxygen species and cellular defense system. Free radicals in human health and disease. Springer; 2015:17-29.
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Linkermann A, Brasen JH, Darding M, et al. Two independent pathways of regulated necrosis mediate ischemia-reperfusion injury. Proceedings of the National Academy of Sciences of the United States of America 2013;110;29:12024-9.
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Broekmans FJ, Soules MR, Fauser BC. Ovarian aging: mechanisms and clinical consequences. Endocr Rev 2009;30;5:465-93.
Ozler A, Turgut A, Soydinc HE, et al. The biochemical and histologic effects of adnexal torsion and early surgical intervention to unwind detorsion on ovarian reserve: an experimental study. Reproductive sciences 2013;20;11:1349-55.
Bristol-Gould SK, Kreeger PK, Selkirk CG, et al. Fate of the initial follicle pool: empirical and mathematical evidence supporting its sufficiency for adult fertility. Developmental biology 2006;298;1:149-54.
Calis P, Bozdag G, Karakoc Sokmensuer L, Kender N. Does ischemia-reperfusion injury affect ovarian reserve and follicle viability in a rat model with adnexal torsion? European journal of obstetrics, gynecology, and reproductive biology 2015;185;126-30.
Demir Caltekin M, Ozkut MM, Caltekin I, et al. The protective effect of JZL184 on ovarian ischemia reperfusion injury and ovarian reserve in rats. The journal of obstetrics and gynaecology research 2021;47;8:2692-2704.
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