Biological characterization of a biodegradable scaffold for common bile duct replacement in an experimental model
DOI:
https://doi.org/10.18203/2320-6012.ijrms20230339Keywords:
Nanotechnology, Nanofiber, Scaffolds, Electrospinning, Tissue engineeringAbstract
Background: The nanofiber scaffolds achieved by the electrospinning technique have been used to develop several biological tissues, the nanofibers obtained by electrospinning procure a favorable microenvironment to mimic the extracellular matrix.
Methods: Study type was of experimental. Study conducted at National Autonomous University of Mexico, from May 2018- May 2022. The protocol was approved by the research and ethics commissions of the UNAM school of medicine. A viscoelastic solution of polylactic-co-glycolic acid (PLGA) and polycaprolactone (PCL) in a 70:30 ratio and gelatin (Gel) in an 80:20 ratio was prepared while a dynamic collector was used with the electrospinning technique.
Results: Mechanical and biological tests were carried out on the scaffold obtained by electrospinning; the resultant scaffold achieves good mechanical matching and structural similarity between the graft and the extrahepatic bile duct.
Conclusions: In this study we managed to create a porous, biocompatible scaffold with good cell adhesion and proliferation, potentially applicable to tissue engineering, especially for the replacement of tubular organs such as blood vessels, bile ducts, and urethra.
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