Published: 2016-12-18

Bacteriological profile isolated from cases of otitis media diagnosed at tertiary care hospital of Gujarat, India

Kirankumar Navnitlal Patel, Gaurishanker P. Shrimali, Nilesh Thakor


Background: Otitis media is commonest disease seen in children. Bacteria are responsible for majority of the cases of otitis media. The aim of the study was to speculate the aetiological bacterial flora which was responsible for the cases of otitis media.

Methods: The present study was conducted by Department of Microbiology, B. J. Medical College, Ahmedabad, India during 1st May 2000 to 30th April 2002. Before conducting the study approval was obtained from Institutional Ethical Committee for human research. Total 140 patients of otitis media were included after written informed consent. The ear discharge from each diseased middle ear was collected and inoculated on blood agar, MacConkey’s agar and chocolate agar media and were incubated at 37°C for 24 hours. The organisms were identified by using a standard procedure. Data was analyzed using Microsoft exel software (Trial Version).

Results: Out of 140 samples 114 (81.43%) had positive culture. out of 114 cases, 64 (56.14%) were male. Majority of the cases (32.45%) were in the age group of 0-14 years. Out of 114 positive cases, single organism was isolated in 99 (86.8%) cases. Out of total 116 strains 44 (37.9%) were gram positive and 72 (62.1%) were gram negative organisms. Among gram positive organisms, Staphylococcus aureus (63.63%) was predominant organisms isolated followed by coagulase negative Staphylococci (20.45%) and among gram negative organism Pseudomonas (69.44%) was predominant organisms isolated followed by proteus species.

Conclusions: Staphylococcus aureus and Pseudomonas sp. were found to be the common cause of otitis media in our study.



Otitis media, Bacterial flora, Staphylococcus aureus, Pseudomonas, Middle ear

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Nussinovitch M, Rimon A, Volovitz B, Raveh E, Prais D, Amir J. Cotton-tip applicators as a leading cause of otitis externa. Int J Pediatr Otorhinolaryngol. 2004;68:433-5.

Brook I, Saantosa G. Microbiology of chronic suppurative otitis media in children in Surabaya, Indonesia. Int J Peditr Otorhnolarygol. 1995;31:23-8.

Verhoeff M, Van Veen EL, Rovers MM, Sanders EA, Schilder AG. Chronic suppurative otitis media: a review. Int J Pediatr Otorhinolaryngol. 2006;70:1-12.

Bluestone CD, Klein JO. Microbiology. In: Bluestone CD, Klein JO, eds. Otitis Media in Infants and Children. 3rd ed. Philadelphia, PA: W. B. Saunders; 2001:79-1014.

Ologe FE, Nwawolo CC. Chronic suppurative otitis media in school pupils in Nigeria. East Afr Med J. 2003;80:130-4.

Daly A. Knowledge and attitude about otitis media risk: implication for prevention. J Pediatr. 1997;3(100):93-6.

Ahmad A, Usman J, Hashim R. Isolates from chronic suppurative otitis media and their antimicrobial sensitivity. Pak Armed Forces Med J. 1999;49:82-5.

Arol B. Antibiotics for upper respiratory tract infection. J Respir Med. 2005;99(3):250-5.

Collee JG, Duguid JP, Fraser AG, Marmion BP, Simmons A. Laboratory strategy in the diagnosis of infective syndromes. Mackie and McCartney Practical Medical Microbiology. Churchill Livingstone: Singapore. 14th ed. 1996:53-94.

Shyamala R, Reddy PS. The study of becteriologigal agents of chronic suppurative otitis media: Aerobic culture and evaluation. J Microbiol biotech Res. 2012;2:152-62.

Gulati J, Tandon PL, Singh W, Bais AS. Study of bacterial flora in chronic suppurative otitis media. Indian J Otolaryngol Head Neck Surg. 1969;2:198-202.

Loy AH, Tan AL, Lu PK. Microbiology of chronic suppurative otitis media in Singapore. Singapore Med J. 2002;43:296-9.

Agrawal A, Kumar D, Goyal A, Goyal S, Singh N, Khandelwal G. Microbiological profile and their antimicrobial sensitivity pattern in patients of otitis media with ear discharge. Indian J Otol. 2013;19:5-8.

Vijaya D, Nagarathnamma T. Microbiological study of chronic suppurative otitis media. Indian J Otol 1998;4:172-4.

Fatma AA, Assiry S, Siraj MZ. Microbiological evaluation and aspects on management of chronic suppurative otitis media in Riyadh. Indian J Otol. 1998;4:115-20.

Chakraborty A, Bhattacharjee A, Purkaystha P. Microbiological profile of chronic suppurative otitis media: Its significance in North-East India. Indian J Otol. 2005;11:39-44.

Taneja M, Taneja MK. CSOM a bacteriological study. Indian J Otol. 2009;15:3-7.

Kuchhal V. Antibiotic sensitivity pattern in chronic suppurative otitis media in Kumoun region. Indian J Otol. 2010;16:17-21.

Shyamla R, Reddy SP. The study of bacteriological agents of chronic suppurative otitis media-aerobic culture and evaluation. J Microbiol Biotechnol Res. 2012;2:152-62.

Sharma S, Rehan HS, Goyal A, Jha AK, Upadhayaya S, Mishra SC. Bacteriological profile in chronic suppurative otitis media in Eastern Nepal. Trop Doct. 2004;34:102-4.

Aslam MA, Ahmed Z, Azim R. Microbiology and drug sensitivity patterns of chronic suppurative otitis media. J Coll Physicians Surg Pak.2004;14:459-61.