Study of impairment in collaboration between ceruloplasmin and transferrin in development of complications in type 2 diabetes mellitus

Authors

  • Reenu Sharma Department of Biochemistry, GMERS Medical College, Sola, Ahmedabad, Gujarat
  • Sunil S. Patani Department of Biochemistry, GMERS Medical College, Sola, Ahmedabad, Gujarat
  • Maulik S. Nayak Department of Biochemistry, GMERS Medical College, Sola, Ahmedabad, Gujarat

DOI:

https://doi.org/10.18203/2320-6012.ijrms20162338

Keywords:

Type 2 diabetes mellitus, Ceruloplasmin, Transferrin, Malondialdehyde

Abstract

Background: Hyperglycemia in diabetic patients can increase the levels of free radicals through various metabolic alterations. One of the most reactive pro-oxidants in peroxidation reactions is free, redox-active iron and generates highly reactive hydroxyl radicals that initiate lipid peroxidation. The purpose of the study was to elucidate the role of CP and TRF in type 2 DM and analyse the effect of disturbance in collaboration between these parameters in pathogenesis of type 2 diabetic complications.

Methods: We included 100 Type 2 DM subjects (with and without complications) and 100 healthy controls. The duration of type 2 DM in study subjects ranged from 5 to 16 years.  Blood samples in fasting condition were collected for analysis of serum malondialdehyde (MDA), CP and TRF.

Results: In the study serum CP levels were higher in Type 2 diabetic subjects with complications compared to diabetic subjects without complications (P=0.01). However, significantly low transferrin values were obtained in diabetic subjects with complications compared to diabetic subjects without complications (P=0.007). Significantly high MDA levels were observed (P=0.002) in type 2 diabetic subjects with complications compared to type 2 diabetic subjects without complications.

Conclusions: The results of the present study indicate oxidative stress plays a role in precipitating complications in Type 2 DM reflecting in disturbance of CP and TRF collaboration.

 

References

Sicree R, Shaw J, Zimmet P. Diabetes and impaired glucose tolerance in India Diabetes Atlas. Gan D Ed. International Diabetes Federation, Belgium. 2006;15-103.

Ramakrishna V, Jailkhani R. Oxidative stress in non-insulin dependent diabetes mellitus (NIDDM) patients. Acta Diabetol. 2008;45:41-6.

Campenhout AV, Campenhout CV, Lagrou AR, Moorkens G, De Block C, Manuel-y-Keenoy B. Iron binding antioxidant capacity is impaired in diabetes mellitus. Free Radical Biology and Medicine. 2006;40:1749-55.

Wolff S, Dean RT. Fragmentation of proteins by free radicals and its effect on their susceptibility to enzymic hydrolysis. Biochem J. 1986;234:399-403.

Esterbauer H, Schaur RJ, Zollner J. Chemistry and Biochemistry of 4-hydroxynonenal malondialdehyde and related aldehydes (Review). Free Radic Biol Med. 1991;11:82-128.

Tuomainen TP, Nyyssonen K, Salonen R, Tervahauta A, Korpela H, Lakka T. Body iron stores are associated with serum insulin and blood glucose concentrations. Population study in 1,013 eastern Finnish men. Diabetes Care. 1997;20(3): 426-428.

Fernandez-Real JM, Lopez-Bermejo A, Ricart W. Cross talk between iron metabolism and diabetes. Diabetes. 2002;51(8):2348-54.

Gutteridge JM, Winyard PG, Blake DR. The behaviour of caeruloplasmin in stored human extracellular fl uids in relation to ferroxidase II activity,lipid peroxidation and phenanthroline- detectable copper. Biochem J. 1985;230(2):517-23.

Singh R, Barden A, Mori T, Beilin L. Advanced glycation endproducts: a review. Diabetologia 2001;44(2):129-46.

Satoh K. Serum lipid peroxide in cerebrovascular disorders determined by a new colorimetric method. Clin Chem Acta. 1978;90:37-43.

Downloads

Published

2017-01-04

How to Cite

Sharma, R., Patani, S. S., & Nayak, M. S. (2017). Study of impairment in collaboration between ceruloplasmin and transferrin in development of complications in type 2 diabetes mellitus. International Journal of Research in Medical Sciences, 4(8), 3611–3615. https://doi.org/10.18203/2320-6012.ijrms20162338

Issue

Section

Original Research Articles