Published: 2017-08-26

Hepatitis B virus infection and ABO/Rh blood groups

Ozlem Genc


Background: Chronic HBV infection is a significant public health problem all around the world. It is not clear if ABO/Rh blood groups have a role in the development of chronic forms of hepatitis B virus (HBV) infection. The aim of the study was to investigate the relationship between chronic HBV infection and ABO/Rh blood groups.

Methods: The study was designed as a retrospective study that included totaled 937 individuals: 453 patients diagnosed with chronic hepatitis and 484 healthy individuals as the control group. HBsAg (hepatitis B surface antigen) and blood groups results of patients between the years 2013-2015 were collected by reviewing the laboratory results. During these three years HBsAg was performed on the architect i2000sr (Abbott diagnostic, Illinois, USA) with ELISA and ABO/Rh blood groups were analyzed with gel centrifugation method (Grifols, Barcelona, Spain).

Results: Blood group A Rh positive was higher than other blood types in the chronic hepatitis B group (44.3%) and in control group (41.9%), whereas blood types O, AB, and B were similar between cases with chronic hepatitis and controls (p>0.05). HBV infection was moderately less frequent in subjects with AB positive blood group (p=0.07). The prevalence of Rh positive was 89.1% in patients and 88.2% in the control group (p>0.05).

Conclusions: This study showed that there was not an association between ABO/Rh blood groups and chronic HBV infection, but there is a need for different and more numerous case-control studies about this subject.


ABO blood group, Hepatitis B virus, Rh blood group

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Anstee DJ. The relationship between blood groups and disease. Blood. 2010;115(23):4635-43.

Liumbruno GM, Franchini M. Beyond immunohematology: the role of ABO blood group in human disease. Blood Transfus. 2013;11:491-9.

Storry JR, Castilho L, Daniels G, Flegel WA, Garratty G, de Haas M, et al. International society of blood transfusion working party on red cell immunogenetic and blood group terminology: Cancun report (2012). Vox Sang. 2014;107:90-6.

Ravn V, Dabelsteen E. Tissue distribution of histo-blood group antigens. APMIS. 2000;108:1-28.

Anstee DJ. The functional importance of blood group-active molecules in human red blood cells. Vox Sang. 2011;100:140-9.

Yamamoto F, Cid E, Yamamoto M, Blancher A. ABO research in the modern era of genomics. Transfus Med Rev. 2012;26:103-18.

World Health Organization. Hepatitis B factsheet number 204 (updated March 2015). Available at

Toy M, Onder FO, Wormann T, Bozdayi AM, Schalm SW, Borsboom GJ, et al. Age and region-specific hepatitis B prevalence in Turkey estimated using generalized linear mixed models: a systematic review. BMC Infect Dis. 2011;11:337.

Salduz ZI, Cetin G, Karatoprak C, Ozder A, Bilginc M, Gultepe I, et al. ABO and Rh blood group distribution in Istanbul Province (Turkey). Istanbul Med J. 2015;16:98-100.

Pourhassan, A. Association between ABO Blood/Rhesus Grouping and hepatitis B and C: a case-control study. Pak J Biol Sci. 2014;17(6):868-71.

Naeini AE, Rostami M, Naeini SE. Chronic viral hepatitis and their relation to ABO blood groups and rhesus (Rh) factor. Med Case Stud. 2010;1(1):5-7.

Mohammadali F, Pourfathollah A. Association of ABO and Rh blood groups to blood-borne infections among blood donors in Tehran-Iran. Iran J of Public Health. 2014;43(7):981-9.

Joshi SK, Ghimire GR. Serological prevalence of antibodies to human immunodeficiency virus (HIV) and hepatitis B virus (HBV) among healthy Nepalese males- a retrospective study. Kathmandu Univ Med J. 2003;1(4):251-5.

Anwar MS, Sıddıqı GM, Haq S, Khokhar NG. Association of blood group types to hepatitis B and hepatitis C virus infection. Biomedica. 2011;27:57-61.

Behal R, Jain R, Behal KK, Bhagoliwal A, Aggarwal N, Dhole TN. Seroprevalence and risk factors for hepatitis B virus infection among general population in Northern India. Arq Gastroenterol. 2008;45(2):137-40.

Sreedhar Babu KV, Bandi S, Kondareddy S, Madithadu A. Association of ABO and Rh blood groups to HBV, HCV infections among blood donors in a blood bank of tertiary care teaching hospital in Southern India: A retrospective study. Int J Res Med Sci. 2015;3(7):1672-6.

Siransy LK, Nanga ZY, Zaba FS, Tufa NY, Dasse SR. ABO/Rh blood groups and risk of HIV infection and hepatitis B among blood donors of Abidjan, Cote D’Ivoire. Eur J Microbiol Immunol. 2015;5(3):205-9.

Kumar MR, Rao MS, Pulıcherla K, Ghosh M. Studies on the distribution of hepatitis B (HBV) and human immunodeficiency virus (HIV)- their relation to blood groups and rhesus (Rh) factor in Guntur district of Andhra Pradesh, India. Asian J Pharm Clin Res. 2013;6(1):109-11.

Tyagi S, Tyagi A. Possible correlation of transfusion transmitted diseases with Rh type and ABO blood group system. JCDR. 2013;7(9):1930-1.

Das S, Kumar MH. Association of blood group types to hepatitis B and hepatitis C viruses among blood donors. A five years institutional based study. Int J Appl Basic Med Sci. 2012;2(1):191-5.

Nigam JS, Singh S, Kaur V, Giri S, Kaushal RP. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system. Hematol Rep. 2014;6(4):560-2.

Dirisu JO, Alli TO, Adegoke AO, Osazuwa F. A survey of prevalence of serum antibodies to human immunodeficiency virus (HIV), hepatitis B virus (HBV) and hepatitis C virus (HCV) among blood donors. N Am J Med Sci. 2011;3(1):35-8.

Ahmad J, Taj AS, Rahim A, Shah A, Rehman M. Frequency of hepatitis B and hepatitis C in healthy blood donors of NWFP: a single center experience. JPMI. 2011;18(3):343-52.