Comparative evaluation of storage time on the quality of platelet concentrates prepared from buffy coat at ambient temperature
DOI:
https://doi.org/10.18203/2320-6012.ijrms20232791Keywords:
Buffy coat, Fresh buffy coat platelet concentrates, Overnight buffy coat platelet concentrates, Platelet concentratesAbstract
Background: Buffy coat-platelets concentrates (BC-PC) are prepared within 6-8 hours of blood collection with 1-2 hours of hanging period which imposes time constraints and logistic problems when collecting blood from blood donation camps in wide geographic areas.
Methods: In our prospective study of one year at Dayanand Medical College and Hospital, Ludhiana we prepared 50 units of BC-PC after hanging BC for 2 hours and another 50 after storing BC O/N at ambient temperature. Platelet (plt) count, WBC contamination and other biochemical parameters in both groups of PC were analyzed on day 1 and day 5 of preparation.
Results: The mean plt counts of O/N BC-PC on day 1 was 8.36±1.22 × 1010, significantly higher than that of F (fresh) BC-PC (7.44±0.81 × 1010). On day 5, F BC-PC showed 6.21±0.88 × 1010 count while it was 6.87±0.96 × 1010 in O/N BC-PC. WBC contamination was lower in O/N BC-PC. On day 1, in F BC-PC WBC contamination was observed as 4.44±2.02 × 107 while in O/N BC-PC it was 3.19±3.33 × 107. On day 5, in F BC-PC WBC contamination was observed as 3.77±0.66 × 107 while in O/N BC-PC it was 2.92±1.71 × 107. Results of biochemical parameters (pH, pO2, pCO2, glucose) were significantly higher and better in F BC-PC but both methods of preparation provided plts optimal survival conditions throughout storage period.
Conclusions: O/N BC-PC provides a better-quality product while solving logistic problems.
References
Moroff G, Holme S, Heaton WA, Kevy S, Jacobson M, Popovsky M. Effect of an 8‐hour holding period on in vivo and in vitro properties of red cells and factor VIII content of plasma after collection in a red cell additive system. Transfusion. 1990;30(9):828-32.
Saran RK, editor. Transfusion medicine: technical manual. 2nd edn. Directorate General of Health Services, Ministry of Health and Family Welfare, Government of India; 2003:7-14.
Guidelines for blood donor selection and Blood donor referral. 2017 by Government of India Ministry of Health and Family Welfare National Aids Control Organization. National Blood Transfusion Council; 2017:5-11.
Rinder HM, Smith BR. In vitro evaluation of stored platelets: is there hope for predicting posttransfusion platelet survival and function? Transfusion. 2003;43(1):2-6.
Norol F, Bierling P, Roudot-Thoraval F, Le Coeur FF, Rieux C, Lavaux A, et al. Platelet transfusion: a dose-response study. Blood. 1998;92(4):1448-53.
Boeri N, Saleun S, Pelissier E, Saleun JP, Aiach M, Rendu F. Influence of a 12‐hour, 22°C holding period for buffy coats on the preparation of platelet concentrates stored in plasma. Transfusion. 1994;34(10):881-6.
Dijkstra-Tiekstra MJ, Kuipers W, Setroikromo AC, deWildt-Eggen J. Overnight or fresh buffy coat-derived platelet concentrates prepared with various platelet pooling systems. Transfusion. 2008;48(4):723-30.
DijkstraTiekstra MJ, vander Meer PF, Cardigan R, Devine D, Prowse C, Sandgren P, et al. Platelet concentrates from fresh or overnight stored blood, an international study. Transfusion. 2011;51:38S-44S.
Philip J, Samantha K, Chatterjee T, Biswas AK, Mallhi RS. Evaluation of random donor platelets produced from buffy coats stored for 24 hr at ambient temperature: should this be implemented in India? Indian J Hematol Blood Transfus. 2015;31(2):264-8.
Rácz Z, Baróti C. Storage of platelet concentrates from overnight-stored blood and overnight-stored buffy coat: in vitro studies. Vox Sang. 1995;68(3):160-3.
Pérez-Pujol S, Lozano M, Perea D, Mazzara R, Ordinas A, Escolar G. Effect of holding buffy coats 4 or 18 hours before preparing pooled filtered PLT concentrates in plasma. Transfusion. 2004;44(2):202-09.
Bӧck M, Rahrig S, Kunz D, Lutze G, Heim U. Platelet concentrates derived from buffy coat and apheresis: biochemical and functional differences. Tranf Med. 2002;12:317-24.
Keegan T, Heaton A, Holme S, Owens M, Nelson E, Carmen R. Paired comparison of platelet concentrates prepared from platelet rich plasma and buffy coats using a new technique with 111In and 51Cr. Transfusion. 1992;32(2):113-20.
Murphy S, Kahn RA, Holme S, Phillips GL, Sherwood W, Davisson W, et al. Improved storage of platelets for transfusion in a new container. Blood. 1982;60(1):194-200.
Adam GA, Swenson SD, Rock G. 5-day storage of human platelet concentrates in 30 ml of plasma or artificial medium. Vox Sang. 1987;52(4):305-12.
Hirosue A, Yamamoto K, Shiraki H, Kiyokawa H, Maeda Y, Yoshinari M. Preparation of white-cell-poor blood components using a quadruple bag system. Transfusion. 1988;28(3):261-4.
