Seroprevalence of hepatitis B surface antigen and antibody to hepatitis C virus at a tertiary care centre in Telangana

Purti Chandrashekher Tripathi, Trinain Kumar Chakraverti, Nileshkumar Ramniklal Khant


Background:Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV) are serious public health problem worldwide and major causes of chronic hepatitis, liver cirrhosis and hepatocellular carcinoma. The purpose of this study was to estimate the prevalence rates of HBV and HCV infections in this part of country.

Methods:Serum samples of inpatients and outpatients were collected over a period of one and a half year. HBsAg was determined using the HBsAg one step (HEPACARD) hepatitis B surface antigen test device. Antibody detection of HCV was done using HCV TRI-DOT.

Results:A total of 4369 serum samples were tested for HBsAg detection and 736 serum samples were tested for hepatitis C virus antibodies. Seropositivity for HBsAg was 1.69% whereas HCV seropositivity was 0.4%. A higher seroprevalence of HBsAg and HCV was found in males as compared with females.

Conclusion:Attempts should be made to reduce the incidence of HCV and HBV and their unregulated spread which can be done by increasing public awareness of simple preventive measures.


Hepatitis B virus (HBV), Hepatitis C virus (HCV), Seroprevalence

Full Text:



Horvat RT, Tegtmeier GE. Hepatitis B, D viruses. Manual of Clinical Microbiology. In: Murray PR, Baron EJ, Jorgensen JH, Pfaller M, Yolken RH, eds. 8th ed. Washington DC: ASM Press; 2003.

Kowdley KV. The cost of managing chronic hepatitis B infection, a global perspective. J Clin Gastroenterol. 2004;38(10 Suppl):s132-3.

Tandon BN, Acharya SK, Tandon A. Epidemiology of HBV & HCV in India. Int Hepatol Communicat. 1996;5;14-8.

Tandon BN, Gandhi BM, Joshi YK. Etiological spectrum of viral hepatitis & prevalence of markers of hepatitis A & B virus infection in north India. Bull WHO. 1984;62;67-73.

Shepard CW, Finelli L, Alter MJ. Global epidemiology of hepatitis C virus infection. Lancet Infect Dis. 2005;5:558-67.

Kenneth WL, Jeffrey TK. Hepatitis B. American Family Physician (Electronic version), 2004. Available at:

Hoofnagle JH, Di Bisceglie AM. Serologic diagnosis of acute and chronic viral hepatitis. Semin Liver Dis. 1991;11:73.

Lee JM, Ahn SH. Quantification of HBsAg: basic virology for clinical practice. World J Gastroenterol. 2011;17:283-9.

Sato K, Ichiyama S, Iinuma Y, Nada T, Shimokata K, Nakashima N. Evaluation of immunochromatographic assay systems for rapid detection of hepatitis B surface antigen and antibody, Dainascreen HBsAg and Dainascreen Ausab. J Clin Microbiol. 1996;34:1420-2.

Ansari MHK, Omrani MD, Movahedi V. Comparative evaluation of immunochromatographic rapid diagnostic tests and PCR methods for detection of human hepatitis B surface antigen. Hepatitis Monthly. 2007;7:87-91.

Clement F, Dewint P, Leroux-Roels G. Evaluation of a new rapid test for the combined detection of hepatitis B virus surface antigen and hepatitis B virus e antigen. J Clin Microbiol. 2002;40:4603-6.

Collee JG, Fraser AG, Marmion BP, Simmons A. HBV immunization. In: Collee JG, Fraser AG, Marmion BP, Simmons A, eds. Mackie and McCartney Practical Medical Microbiology. 14th ed. New York: Churchill-Livingstone; 1999.

Lodha R, Jain Y, Anand K, Kabra SK, Pandav CS. Hepatitis B in India: a review of disease epidemiology. Indian Pediatr. 2001;38:1318-22.

Chaudhary A. Epidemiology of hepatitis B virus in India. Hep B Annual. 2004;1:17-24.

Sood S, Malvankar S. Seroprevalence of hepatitis B surface antigen, antibodies to the hepatitis C virus, and human immunodeficiency virus in a hospital-based population in Jaipur, Rajasthan. Indian J Community Med. 2010;35:165-9.

Batham A, Narula D, Toteja T, Srenivas V, Puliyel JM. Systemic review of meta-analysis of prevalence of hepatitis B in India. Indian Pediatr. 2007;44(9):663-74.

Srikrishna A, Sitalakshmi S, Damodar P. How safe are our safe donors? Indian J Pathol Microbiol. 1999;42:411-6.

Dutta S, Shivanand PG, Chatterjee A. Prevalence of hepatitis B surface antigen and antibody among hospital admitted patients in Manipal. Indian J Public Health. 1994;38:108-12.

Singh K, Bhat S, Shastry S. Trend in seroprevalence of hepatitis B virus infection among blood donors of coastal Karnataka. India J Infect Dev Ctries. 2009;3(5):376-9.

Qamer S, Shahab T, Alam S, Malik A, Afzal K. Age specific prevalence of hepatitis B surface antigen in pediatric population of Aligarh, North India. Indian J Pediatr. 2004;71:965-7.

Chameera EWS, Noordeen F, Pandithasundara H, Abeykoon AMSB. Diagnostic efficacy of rapid assays used for the detection of hepatitis B virus surface antigen. Sri Lankan J Infect Dis. 2013;3(2):21-7.

Lin Y, Wang Y, Loua A, Day G, Qiu Y, Nadala Jr ECB, et al. Evaluation of a new hepatitis B virus surface antigen rapid test with improved sensitivity. J Clin Microbiol. 2008;46:3319-24.

Mishra S, Chayani N, Sarangi G, Mallick B, Pati SB. Seroprevalence of anti HCV antibody in and around Cuttack, Orissa. Indian J Med Microbiol. 2002;20:40-1.

Bhattacharya S, Badrinath S, Hamide A, Sujatha S. Seroprevalence of hepatitis C virus in a hospital based general population in South India. Indian J Med Microbiol. 2003;21:43-5.

Ramarokoto CE, Rakotomanana F, Ratsitorahina M, Raharimanga V, Razafindratsimandresy R, Randremanana R, et al. Seroprevalence of hepatitis C and associated risk factors in urban areas of Antananarivo, Madagascar. BMC Infect Dis. 2008;8:25.