Evaluation of efficacy of an eco friendly enviropap and REAP stain in cervical cytology at resource limited area


  • Supreetha Megalamane Department of Pathology, Sri Devaraj Urs Medical, Kolar, Karnataka, India
  • Nanda Radhakrishna Leena Sri Devaraj Urs Medical, Kolar, Karnataka, India




Cytological technique, Cancer screening, Enviro pap, Pap smear


Background: Cervical cancer is integral to any cancer control program in India because of the disease burden. Quick diagnosis plays a crucial role in limited resource areas. Hence, cost-effective and eco-friendly staining procedures with less time consumption help screening for cervical cancer cytodiagnosis. Thus, we wanted to assess the superiority of staining of smears by REAP (Rapid Economical Acetic acid Papanicolaou) technique and the newer EnviroPap (Environmental Papanicolaou) technique compared to conventional Pap technique on cervical smears.

Methods: From each patient, three smears were prepared, one with routine conventional method, one with REAP stain, and another with EnviroPap stain and quality parameters were noted. The chi-square test assessed differences in categorical variables between stains; a p value of <0.05 was considered significant. Statistical analysis used was the Chi-square test, and p value of <0.05 using a two-tailed test was taken as being significant for all statistical tests.

Results: REAP and Enviropap stained smears showed excellent nuclear and cytoplasmic details compared to conventional Pap staining protocol.

Conclusions: REAP and EnviroPap staining protocols for those two techniques were simpler, easier, and cost-effective, which took minimal turnaround time for assessing pap smears at a large scale.


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How to Cite

Megalamane, S., & Leena, N. R. (2023). Evaluation of efficacy of an eco friendly enviropap and REAP stain in cervical cytology at resource limited area. International Journal of Research in Medical Sciences, 11(11), 4095–4101. https://doi.org/10.18203/2320-6012.ijrms20233381



Original Research Articles