Comparative evaluation of non enzymatic antioxidants, vitamin C and albumin in patients suffering from different grades of periodontitis: a retrospective observational study
Keywords:Antioxidants, Periodontitis, Serum
Background: Rate of degree of destruction of periodontium may be associated with the oxidative stress due to the imbalance of different serum antioxidants. The objective of this study is to compare the evaluated concentrations of non enzymatic antioxidants like vit-C, albumin levels and total antioxidant status, from the available data of patients with slowly and rapidly progressive periodontitis (RPP).
Methods: This retrospective observational study was confined to a study population, which include; 42 patients with RPP, 42 patients with slowly progressive periodontitis (SPP) and 86 healthy controls, in whom the required data were available. The levels of non enzymatic antioxidants like albumin, vitamin C and total antioxidant capacity (TAC) of patients, suffering from RPP, are compared with SPP and healthy controls.
Results: The values of serum albumin and vitamin-c levels in RPP even remaining within the normal ranges were found to be on the lower side, when compared with healthy controls. The systemic TAC levels were found to be at a lower side in both SPP and RPP, when compared with the healthy control group.
Conclusions: The systemic levels of non enzymatic antioxidants, vit C and albumin, in RPP were found to be on the lower side, even remaining within the normal ranges, when compared with the healthy controls. The TAC in SPP and RPP is also found to be on lower side, consistent with previous studies.
Tatakis DN, Kumar PS. Etiology and pathogenesis of periodontal diseases. Dent Clin North Am. 2005;49(3):491-516.
Tóthová L, Kamodyová N, Červenka T, Celec P. Salivary markers of oxidative stress in oral diseases. Front Cell Infect Microbiol. 2015;5:73.
Narendra S, Das UK, Tripathy SK, Sahani NC. Superoxide Dismutase, Uric Acid, Total Antioxidant Status, and Lipid Peroxidation Assay in Chronic and Aggressive Periodontitis Patients. J Contemp Dent Pract. 2018;19(7):874-80.
Chapple IL, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000. 2007;43:160-232 .
Baltacıoğlu E, Yuva P, Aydın G, Alver A, Kahraman C, Karabulut E, et al. Lipid peroxidation levels and total oxidant/antioxidant status in serum and saliva from patients with chronic and aggressive periodontitis. Oxidative stress index: a new biomarker for periodontal disease? J Periodontol. 2014;85(10):1432-41.
Shapira L, Gordon B, Warbington M, Van Dyke TE. Priming effect of Porphyromonas gingivalis lipopolysaccharide on superoxide production by neutrophils from healthy and rapidly progressive periodontitis subjects. J Periodontol. 1994;65(2):129-33.
Halliwell B, Gutteridge JC. The definition and measurement of antioxidants in biological systems. Free Radic Biol Med. 1995;18:125-6.
Young IS, Woodside JV. Antioxidants in health and disease. J ClinPathol. 2001;54:176-86.
Miller NJ, Rice-Evans C, Davies MJ, Gopinathan V, Milner A. A novel method for measuring antioxidant capacity and its application to monitoring the antioxidant status in premature neonates. Clin Sci 1993;84:407-12.
Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol 2001;54(5):356-61.
Benzie IF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of ‘‘antioxidant power’’: the FRAP assay. Anal Biochem. 1996;239:70-6.
Benzie IF, Strain JJ. Ferric reducing/antioxidant power assay: direct measure of total antioxidant capacity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods Enzymol 1999;299:15-27.
Rice-Evans C, Miller NJ. Total antioxidant status in plasma and body fluids. Methods Enzymol. 1994;234:279-93
Tsai CC, Chen HS, Chen SL, Ho YP, Ho KY, Wu YM, et al. Lipid peroxidation: a possible role in the induction and progression of chronic periodontitis. J Periodontal Res. 2005;40(5):378-84.
Moore S, Calder KA, Miller NJ, Rice-Evans CA. Antioxidant activity of saliva and periodontal disease. Free Radic Res. 1994;21(6):417-25.
Guarnieri C, Zucchelli G, Bernardi F, Scheda M, Valentini AF, Calandriello M. Enhanced superoxide production with no change of the antioxidant activity in gingival fluid of patients with chronic adult periodontitis. Free Radic Res Commun. 1991;15(1):11-6.
Van Dyke TE, Zinney W, Winkel K, Taufiq A, Offenbacher S, Arnold RR. Neutrophil function in localized juvenile periodontitis. Phagocytosis, superoxide production and specific granule release. J Periodontol. 1986;57(11):703-8.
Waddington RJ, Moseley R, Embery G. Reactive oxygen species: a potential role in the pathogenesis of periodontal diseases. Oral Dis. 2000;6(3):138-51.
Chapple IL, Matthews JB. The role of reactive oxygen and antioxidant species in periodontal tissue destruction. Periodontol 2000. 2007;43:160-232.
Baser U, Gamsiz-Isik H, Cifcibasi E, Ademoglu E, Yalcin F. Plasma and salivary total antioxidant capacity in healthy controls compared with aggressive and chronic periodontitis patients. Saudi Med J. 2015;36:856-61.
Chapple IL, Milward MR, Dietrich T. The prevalence of inflammatory periodontitis is negatively associated with serum antioxidant concentrations. J Nutr. 2007;137(3):657-64.
Dodington DW, Fritz PC, Sullivan PJ, Ward WE. Higher Intakes of Fruits and Vegetables, β-Carotene, Vitamin C, α-Tocopherol, EPA, and DHA Are Positively Associated with Periodontal Healing after Nonsurgical Periodontal Therapy in Nonsmokers but Not in Smokers. J Nutr. 2015;145(11):2512-9.
Erel O. A novel automated direct measurement method for total antioxidant capacity using a new generation, more stable ABTS radical cation. Clin Biochem. 2004;37:277-85.
Robitaille L, Hoffer LJ. A simple method for plasma total vitamin C analysis suitable for routine clinical laboratory use. Nutr J. 2016;15:40.
Kawai Y, Masutani K, Torisu K. Association between serum albumin level and incidence of end-stage renal disease in patients with Immunoglobulin A nephropathy: A possible role of albumin as an antioxidant .PLoS One. 2018;13(5).
Duly EB, Grimason S, Grimason P, Barnes G, Trinick TR. Measurement of serum albumin by capillary zone electrophoresis, bromocresol green, bromocresol purple, and immunoassay methods. J Clin Pathol. 2003;56(10):780-1.
Sculley DV, Langley-Evans SC. Salivary antioxidants and periodontal disease status. Proc Nutr Soc. 2002;61(1):137-43.
Akalin FA, Baltacioğlu E, Alver A, Karabulut E. Lipid peroxidation levels and total oxidant status in serum, saliva and gingival crevicular fluid in patients with chronic periodontitis. J Clin Periodontol. 2007;34(7):558-65.
Sheikhi M, Bouhafs RK, Hammarström KJ, Jarstrand C. Lipid peroxidation caused by oxygen radicals from Fusobacterium-stimulated neutrophils as a possible model for the emergence of periodontitis. Oral Dis. 2001;7(1):41-6.
Kimura S, Yonemura T, Kaya H. Increased oxidative product formation by peripheral blood polymorphonuclear leukocytes in human periodontal diseases. J Periodontal Res. 1993;28(3):197-203.
Chapple IL, Milward MR, Ling-Mountford N, Weston P, Carter K, Askey K, et al. Adjunctive daily supplementation with encapsulated fruit, vegetable and berry juice powder concentrates and clinical periodontal outcomes: a double-blind RCT. J Clin Periodontol. 2012;39(1):62-72.
Abou Sulaiman AE, Shehadeh RM. Assessment of total antioxidant capacity and the use of vitamin C in the treatment of non-smokers with chronic periodontitis. J Periodontol. 2010;81(11):1547-54.
Iwasaki M, Yoshihara A, Moynihan P, Watanabe R, Taylor GW, Miyazaki H. Longitudinal relationship between dietary omega-3 fatty acids and periodontal disease. Nutrition. 2010;26:1105-9.
Nishida M, Grossi SG, Dunford RG, Ho AW, Trevisan M, Genco RJ. Dietary vitamin C and the risk for periodontal disease. J Periodontol. 2000;71(8):1215-23.
Ogawa H, Yoshihara A, Amarasena N, Hirotomi T, Miyazaki H. Association between serum albumin and periodontal disease in community-dwelling elderly. J Clin Periodontol. 2006;33:312-6.
Iwasaki M, Yoshihara A, Hirotomi T, Ogawa H, Hanada N, Miyazaki H. Longitudinal study on the relationship between serum albumin and periodontal disease. J Clin Periodontol. 2008;35(4):291-6.