Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to antibiotic agents at super specialty hospital, Amravati city, India

Hrishikesh Sawdekar, Radha Sawdekar, Vinod R. Wasnik

Abstract


Background: Wound infection is one of the health problems that is caused and aggravated by the invasion of pathogenic organisms. Information on local pathogens and sensitivity to antimicrobial agent is crucial for successful treatment of wounds. So the present study was conducted to determine antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to antimicrobial agents.

Methods: A retrospective study was conducted among patients with wound infection in Suyash super speciality hospital, from January 2012 to December 2013. Wound swab was collected using sterile cotton swabs and processed for bacterial isolation and susceptibility testing to Systemic antimicrobial agents.

Results: In this study 78 bacterial isolates were recovered from 258 specimens showing an isolation rate of 31.2%. The predominant bacteria isolated from wounds were gram positive  staphylococci 36 (46.2%), followed by gram negative streptococci 18 (23.1%) gram negative pseudomonas 12 (15.4 %) and gram negative proteus 8 (10.4%). The gram positive and gram negative bacteria constituted 68 (87.2%) and 10 (12.8%) of bacterial isolates; respectively.  

Conclusion: In the present study most of the pathogens isolated from wound isolates showed high rate of resistance to most commonly used newer antibiotics used to treat bacterial infections. Therefore, rational use of antibiotics should be practiced.

 


Keywords


Bacterial isolates, Antimicrobial susceptibility pattern, Drug resistance, Wound infection

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References


Bowler PG, Duerden BI, Armstrong DG. Wound microbiology and associated approaches to wound management. Clin Microbiol Rev. 2001;14:244-69.

Burke JP. Infection control - a problem for patient safety. N Engl J Med. 2003;348:651-6.

Rupp ME, Fey PD. Extended spectrum beta-lactamase (ESBL) - producing Enterobacteriaceae: considerations for diagnosis, prevention, and drug treatment. Drugs. 2003;63:353-65.

Sohil AK, Padma GM, Anand R, Gabriel R. Survey and evaluation of antibiotic prophylaxis usage in surgery wards of tertiary level institution before and after the implementation of clinical guidelines. Indian J Surg. 2006;68:150-6.

Ndip RN, Takang AEM, Echakachi CM, Malongue A, Akoachere J-FTK. In vitro antimicrobial activity of selected honeys on clinical isolates of Helicobacter pylori. Afr Health Sci. 2007;7(4):228-31.

Dai T, Huang Y-Y, Sharma SK, Hashmi JT, Kurup DB, Hamblin MR. Topical antimicrobials for burn wound infections. Recent Pat Antiinfect Drug Discov. 2010;5(2):124-51.

Kotz P, Fisher J, McCluskey P, Hartwell SD, Dharma H. Use of a new silver barrier dressing, ALLEVYN Ag in exuding chronic wounds. Int Wound J. 2009;6:186-94.

Taiwo S, Okesina A, Onile B. In vitro antimicrobial susceptibility pattern of bacterial isolates from wound infections in University of Ilorin teaching hospital. Afr J Clin Exp Microbiol. 2002;3(1):6-10.

Al-Waili NS, Salom K, Al-Ghamdi AA. Honey for wound healing, ulcers, and burns. Sci World J. 2011;11:766-87.

Cooper RA, Molan PC, Harding KG. The sensitivity to honey of gram-positive cocci of clinical significance isolated from wounds. J App Microbiol. 2002;93:857-63.

Dohmen PM, Gabbieri D, Weymann A, Linneweber J, Konertz W. Reduction in surgical site infection in patients treated with microbial sealant prior to coronary artery bypass graft surgery: a case-control study. J Hosp Infect. 2009;72:119-26.

Kühme T, Isaksson B, Dahlin LG. Wound contamination in cardiac surgery. A systematic quantitative and qualitative study of the bacterial growth in sternal wounds in cardiac surgery patients. APMIS. 2007;115:1001-7.

Onche, O. Adedeji. Microbiology of post-operative wound infection in implant surgery. Niger J Surg Res. 2004;6(1):37-40.

Shittu AO, Kolawole DO, Oyedepo EA. A study of wound infections in two Health Institutions in Ile-Ife, Nigeria. Afr J Biomed Res. 2002;5:97-102.

Sonawane J, Kamath N, Swaminathan R, Dosani K. Bacterial profile of surgical site infections and their antibiograms in a tertiary care hospital. Bombay Hosp J. 2010;52:358-61.

Kühme T, Isaksson B, Dahlin LG. Wound contamination in cardiac surgery. A systematic quantitative and qualitative study of the bacterial growth in sternal wounds in cardiac surgery patients. APMIS. 2007;115:1001-7.

Mohammedaman Mama, Alemseged Abdissa, Tsegaye Sewunet. Antimicrobial susceptibility pattern of bacterial isolates from wound infection and their sensitivity to alternative topical agents at Jimma University specialized hospital, South-West Ethiopia. Ann Clin Microbiol Antimicrob. 2014;13:14.

Bhatt C, Lakhey M. The distribution of pathogens causing wound infection and their antibiotic susceptibility pattern. J Nepal Health Res Council. 2006;5(1):22-6.

Mulu A, Moges F, Tessema B, Kassu A. Patterns and multiple drug resistance of bacterial pathogens at university of Gondar teaching hospital. Northwest Ethiopia Ethiop Med J. 2006;44(2):125-31.

Mulu W, Kibru G, Beyene G, Damtie M. Postoperative nosocomial infections and antimicrobial resistance pattern of bacteria isolates among patients admitted at Felege Hiwot referral hospital, Bahirdar. Ethiopia Ethiop J Health Sci. 2012;22(1):7-18.

Emele F, Izomoh M, Alufohai E. Microorganisms associated with wound infection in Ekpoma, Nigeria. West Afr J Med. 1999;18(2):97-100.

Madsen MS, Neumann L, Andersen JA. Penicillin prophylaxis in complicated wounds of hands and feet: a randomized, double-blind trial. Injury. 1996;27(4):275-8.

Thu LT, Dibley MJ, Ewald B, Tien NP, Lam LD. Incidence of surgical site infections and accompanying risk factors in Vietnamese orthopedic patients. J Hosp Infect. 2005;60:360-7.

Maksimovic J, Markovic-Denic L, Bumbasirevic M, Marinković J, Vlajinac H. Surgical site infections in orthopedic patients: prospective cohort study. Croat Med J. 2008.;49(1):58-65.

Markovic DI, Maksimovic J, Lesic A, Stefanovic S, Bumbasirevic M. Etiology of surgical site infections at the orthopedic trauma units. Acta Chir Lugosl. 2009;56(2):81-6.

Comfort Aloysius Etok, Ekom Ndifreke Edem, Ernest Ochang. Aetiology and antimicrobial studies of surgical wound infections in University of Uyo Teaching Hospital (UUTH) Uyo, Akwa Ibom state. Niger Open Access Sci Rep. 2012;1(7):1-5.

Oguachuba HN. Wound Infection in the orthopedic traumatology department of Jos. Niger Med J. 1987;17:147-51.

Gayne RP, Martone WJ, Jarvis WR, Emon TG. CDC definitions of nosocomial surgical site Infections. A modification of CDC definitions of surgical wound infections. Am J Infect Control. 1992;20:271-4.

Onche II. Post-Operative wound infection in implant surgery. In: Onche II, eds. Dissertation. Lagos: National Postgraduate College of Nigeria; 2000.

Mbamali EI. Internal fixation of femoral shaft fractures at the Ahmadu Bello University teaching hospital Zaria. Niger Med Practit. 1981;2:81-5.