A study of biofilm production in clinical isolates of Staphylococci at a tertiary care hospital, Bangalore

Saroj Golia, Asha S. Kamath B, Nirmala AR

Abstract


Background: The Biofilms are densely packed communities of microorganisms consisting of layers of cell clusters embedded in a matrix of extracellular polysaccharide called polysaccharide intercellular adhesin. This layer impedes the delivery of antibiotics to the biofilm forming microbial cells leading to emergence of drug resistance. Staphylococci are commensal bacteria on the human skin and mucous membranes. So it may be easily introduced as a contaminant during the surgical intervention. So, this study was conducted to identify the Biofilm producing strains from clinical isolates of Staphylococci.

Methods: A total of 182 non-repetitive clinical strains of Staphylococci isolated from various clinical samples from Feb 2014 to Oct 2014 were included in the study. All the isolates were identified using standard microbiological procedures. All the samples were tested for biofilm production by modified Congo-red agar method and tube method.

Results: Out of 182 samples that were included in the study, a total of 90 (49.45%) samples showed biofilm formation of which 58 (75.32%) were methicillin resistant and 32 (30.47%) were methicillin sensitive. Also these strains were resistant to other antibiotics.  

Conclusion: Our study showed biofilm production by methicillin resistant strains which were also multidrug resistant. Treatment of methicillin resistant strains of Staphylococci is one of the most challenging task for the clinicians and the microbiologists. So they should be routinely screened for biofilm formation in order to prevent emergence and spread of multidrug resistant strains.

 


Keywords


Biofilm, MRSA

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References


Stewart PS. Mechanisms of antibiotic resistance in bacterial biofilms. Indian J Med Micribiol. 2002;29:107-13.

Michael Otto. Staphylococcal biofilms. Curr Top Microbiol Immunol. 2008;322:207-28.

Thien-Fah C. Mah, George A. O’Toole. Mechanisms of biofilm resistance to antimicrobial agents. Trends Microbiol. 2001 Jan;9(1):34-9.

El-Shekh NA, Ayoub AMA, El-Hendawy HH, Abada EA, Khalifa SYE. In vitro activity of some antimicrobial agents against intact and disrupted biofilms of Staphylococci in the indwelling vascular catheter patients. World Appl Sci J. 2010;10(1):108-20.

CDC. The nosocomial infections surveillance system. Available at: http://www.cdc.gov/ncidod/hip/NNIS/2004NNISreport.pdf

Martinez JL, Baquero F. Mutation frequencies and antibiotic resistance. Antimicrob Agents Chemother. 2000;44(7):1771.

Clinical and Laboratory Standards Institute. Performance standards for antimicrobial susceptibility testing. In: CLSI, eds. Twenty-Fourth Informational Supplement. Wayne, PA, USA: CLSI; 2014: M100-S24.

Christensen GD, Simpson WA, Bisno AL, Beachey EH. Adherence of slime producing strains of staphylococcus epidermidis to smooth surfaces. Infect Immune. 1982;37:318-26.

Freeman DJ, Falkiner FR, Keane CT. New method for detecting slime production by coagulase negative Staphylococci. J Clin Pathol. 1989;42(8):872-4.

Panda PS, Choudhary U, Yadav S, Dube SK. Biofilm production in gram-positive isolates causing urinary tract infection in a tertiary care hospital. Indian J Pathol Microbiol. 2014;57:159-60.

Seema B, Atindra K. Biofilm. A challenge to medical science. J Clin Diagn Res. 2011;5(1):127-30.

Dardi Charan Kaur, Khare AS. Biofilm formation and antibiotic susceptibility pattern in MRSA strains in a tertiary care rural hospital. Indian J Basic Appl Med Res. 2013 Dec;3(1):37-44.

Singh S, Katiyar R, Kaistha SD. High oxacillin, vancomycin and fluoroquinolone resistance amongst biofilm forming Staphylococcus aureus isolates from ulcerative keratitis infections. Indian J Med Microbiol. 2011 Jul-Sep;29(3):312-3.

Sasirekha B, Usha MS, Amruta AJ, Ankit S, Brinda N, Divya R. Evaluation and comparison of different phenotypic tests to detect methicillin resistant Staphylococcus aureus and their biofilm production. Int J PharmTech Res. 2012;4(2):532-41.

Maryam Rezaei, Rezvan Moniri, Seyed Gholam, Abbas Mousavi, Marzie Jabari Shiade. Prevalence of biofilm formation among methicillin resistance Staphylococcus aureus isolated from nasal carriers. Jundishapur J Microbiol. 2013 Aug;6(6):e9601.

Agnes Bedie Eyoh, Michel Toukam, Julius Atashili, Charles Fokunang, Hortense Gonsu, Emilia Enjema Lyonga, et al. Relationship between multiple drug resistance and biofilm formation in Staphylococcus aureus isolated from medical and non-medical personnel in Yaounde, Cameroon. Pan Afr Med J. 2014;17:186.