Impact of Nigella sativa oil on glycemic control in alloxan-induced diabetic Wistar rats

Onahinon Christian; Sunday Ogli; Nndunno Akwaras; Augustine Oko Adugba; Godwin Obochi

doi:10.18203/2320-6012.ijrms20242206

Authors

Onahinon Christian Department of Physiology, Faculty of Basic Medical Science, College of Health Sciences, Benue State University Makurdi, Nigeria
Sunday Ogli Department of Physiology, Faculty of Basic Medical Science, College of Health Sciences, Benue State University Makurdi, Nigeria
Nndunno Akwaras Department of Physiology, Faculty of Basic Medical Science, College of Health Sciences, Benue State University Makurdi, Nigeria
Augustine Oko Adugba Department of Physiology, Faculty of Basic Medical Science, College of Health Sciences, Benue State University Makurdi, Nigeria
Godwin Obochi Department of Biochemistry, Faculty of Basic Medical Science, College of Health Sciences, Benue State University Makurdi, Nigeria

DOI:

https://doi.org/10.18203/2320-6012.ijrms20242206

Keywords:

Black seed oil, Glycosylated hemoglobin insulin

Abstract

Background: To effectively manage diabetes, it is crucial to achieve optimal glycemic control by ensuring that A1C levels remain below 7.0%. This study aimed to investigate the impact of Nigella sativa oil (black seed oil, BSO) on glycemic control in alloxan-induced diabetic Wistar rats.

Methods: Forty (40) male Wistar rats weighing 200-250 gm were randomly allocated into eight (8) groups of five (5) animals per group. Group 1 received normal saline as the normoglycemic control, while groups 2 to 8 were given alloxan monohydrate to induce hyperglycemia, following the method of Osikwe et al. Following the induction of hyperglycemia, group 2 received normal saline, group 3 received 200 mg/kg of metformin, group 4 received 2 mg/kg of glimepiride, group 5 received 2.5 ml/kg of BSO, group 6 received glimepiride and BSO, group 7 received metformin and BSO, and group 8 received BSO, glimepiride, and metformin.

Results: The results showed that BSO significantly reduced fast blood glucose levels compared to the diabetic control group (p<0.05), lowered glycosylated hemoglobin to <7%, and improved pancreatic beta cell function.

Conclusions: Black seed oil reduces fasting blood glucose, exhibits synergism with glimepiride, and improves pancreatic beta-cell function in alloxan-induced diabetic Wistar rats.

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