Serum adenosine deaminase as oxidative stress marker in type 2 diabetes mellitus

Shashikala Magadi Dasegowda, Ashok Kumar Jeppu, Sushith ., Kavitha Ashok Kumar

Abstract


Background: Oxidative stress markers are increased in type 2 diabetes mellitus and its estimation helps in predicting the long term complications. In present study comparison and correlation of the levels of serum adenosine deaminase, serum malondialdehyde, and serum total antioxidant capacity in type 2 diabetes mellitus and in age and sex matched healthy controls.

Methods: Study group consisted of 100 individuals between the age group of 35-65 years of age. Of which 50 individuals with type 2 diabetes mellitus were considered as cases. The control group consisted of 50 age and sex matched healthy individuals. Study was approved by institutional ethical committee. By aseptic precautions 2 ml of venous blood was collected in a plain vacutainer tube, after 8-12 hours of fasting. Serum adenosine deaminase, serum malondialdehyde, and serum total antioxidant capacity were estimated in all groups.

Results: The study observed an increased level of serum adenosine deaminase, malondialdehyde and decreased levels of total antioxidant capacity in type 2 diabetes mellitus compared to controls. Serum adenosine deaminase levels in type 2 diabetics were 50.77 ± 6.95 and in controls was 17.86 ± 4.04. Serum Malondialdehyde levels in type 2 diabetics was 512.13 ± 70.15 and in controls was 239.32 ± 23.97. Serum total antioxidant levels in type 2 diabetics was 0.39±0.15 and in controls was 1.66±0.25. Positive correlation was seen between serum adenosine deaminase and malondialdehyde and it was statistically significant. Statistically significant negative correlation was seen between serum adenosine deaminase and total antioxidant capacity.

Conclusion: Adenosine deaminase can be used as oxidative stress marker. Their increased levels indicate oxidative stress in type 2 diabetes mellitus. Therefore, estimation of serum adenosine deaminase levels help in early prediction and prevention of long term complications occurring due to oxidative stress in diabetics, thereby decreasing the mortality and morbidity in them.

 


Keywords


Serum adenosine deaminase, Type 2 diabetes mellitus, MDA

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References


Nayak BS, Roberts L. Relationship between inflammatory markers, metabolic and anthropometric variables in the Caribbean type-2 diabetic patients with and without microvascular complications. J Inflamm. 2006 Dec;17(3):1-7.

Basu S. Fatty acid oxidation and isoprostanes: oxidative strain and oxidative stress. Prostaglandins, leukotrienes and essential fatty acids. The Ninth Fatty Acids and Cell Signalling Meeting. 2010;82(4-6):219-25.

Tomas Dolezal. Adenosine deaminase: review of physiological roles. In: Tomas Dolezal, eds. A Review Article. Czech Republic: University of South Bohemia; 2001: 2.

Shunsuke Takasuga, Toshiaki Katada, Michio UI, Osamu Hazeki. Enhancement by Adenosine of insulin- induced activation of phoshoinositide 3- kinase and protein kinase B in rat adipocytes. J Biol Chem. 1999;274:1945-50.

John N. Fain, Paul W. Weiser. Effects of adenosine deaminase on cyclic adenosine monophosphate accumulation, lipolysis and glucose metabolism of fat cells. J Biol Chem. 1975;250(3):1027-34.

Giusti G, Galanti B. Direct colorimetric method for the determination of adenosine deaminase and 5-AMP deaminase in the blood. Boll Soc Ital Biol Sper. 1966;42:1316-20.

Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Ann Biochem. 1979;95(2):351-358.53. Ridker P, Cushman M, Stampfer MJ, Tracy RP, Hennekens CH. Inflammation, aspirin, and the risk of cardiovascular disease in apparently healthy men. New Engl J Med. 1997;336(15):973-9.

Benzie FF, Strain JJ. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power. The FRAP assay. Analyt Biochem. 1996;239:70-6.

Siddiqi SS, Ahmad J, Islam N, Ashraf SMK, Mishra SP. A study of the modulation of adenosine deaminase activity in monocytes of type 2 diabetes mellitus by antioxidants. JIACM. 2011;12(2):113-6.

Slatter DA, Bolton CH, Bailey AJ. The importance of lipid-derived malondialdehyde in diabetes mellitus. Diabetologia. 2000;43:550-7.

A. Jamuna Rani, S. V. Mythili. Study on total antioxidant status in relation to oxidative stress in type 2 diabetes mellitus. J Clin Diagn Res. 2014 Mar;8(3):108-10.

Gitanjali G, Sudeep G, Neerja, Mili G, Deepak A, Priyanka S. Effect of hyperglycemia on some biochemical parameters in diabetes mellitus. J Clin Diagn Res. 2010;4:3181-6.