DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20150233

Biocompatibility of nanometre scale porous anodic aluminium oxide membranes towards the RK 13 epithelial cell line: A preliminary study

Gérrard Eddy Jai Poinern, Xuan Thi Le, Mark O’Dea, Derek Fawcett

Abstract


Background: This study for the first time examines the biomedical potential of using anodic aluminium oxide (AAO) for culturing Oryctolagus cuniculus (European Rabbit) Kidney (RK-13) epithelial cells.

Methods: The cellular response of RK-13 cells towards in-house synthesised AAO membranes, a commercially available membrane and glass controls were investigated by examining cell adhesion, morphology and proliferation. The in-house membranes were anodized using a two-step procedure to produce a highly ordered hexagonal pore and channel structure.

Results: Cell proliferation over a 48 h period indicated that the AAO membranes were more than comparable with the glass control substrates. Subsequent microscopy observations revealed evidence of focal adhesion sites and cellular extensions interacting with the underlining porous membrane surface structure.

Conclusions: The study has shown that AAO membranes have the potential to culture RK-13 cells and indicate a possible tissue engineering technique for producing tissues.


Keywords


Nano-porous Anodic Aluminium Oxide, RK-13, Cell Adhesion, Cell Proliferation

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References


Sulka G. Electrochemical Nano structuring of Aluminium based on anodizing and a consecutive electroplating of magnetic material into plates. Katholieke Universiteit; Leuven; Belgium, 2003.

Poinern GEJ, Ali N, Fawcett D. Progress in Nano-Engineered Anodic Aluminium Oxide Membrane Development. Materials. 2011;4:487-526.

Masuda H, Yada K, Osaka A. Self-ordering of cell configuration of anodic porous alumina with large-size pores in phosphoric acid solution. Jpn J Appl Phys. 1998;37:L1340-2.

Shingubara S. Fabrication of nanomaterials using porous alumina templates. J Nanoparticle Res. 2003;5:17-30.

Jirage KB, Hulteen JC, Martin CR. Nanotubule-based molecular filtration membrane. Science. 1997;278:655-8.

Chen J, Xu L, Li W, Gou X. α-Fe2O3 Nanotubes in gas sensor and lithium-ion battery applications. Adv Mater. 2005;17:582-9.

Kim Y, Jung B, Lee H, Kim H, Lee K, et al. Capacitive humidity sensor design based on aluminium oxide. Sens Actuators B. 2009;141:441-6.

Poinern GEJ, Ali N, Berry C, Singh P, Berchmans S, Fawcett D. Biocompatible of synthesised nano-porous anodic aluminium oxide membranes for use as a cell culture substrate for Madin-Darby Canine Kidney cells: A Preliminary Study. J Tissue Sci Eng. 2012;3(3):1-7.

Poinern GEJ, Le X, Hager M, Becker T, Fawcett D. Electrochemical Synthesis and Characterisation of nano-porous anodic aluminium oxide membranes for use as a cell culture substrate for tissue engineering applications. Am J Biomed Eng. 2013;3(6):119-31.

Roach P, Eglin D, Rohde K, Perry C. Modern biomaterials: A review–Bulk properties and implications of surface modifications. J Mater Sci Mater Med. 2007;18:1263-77.

Yim EKF, Leong KW. Significance of nanostructures in dictating cellular response. Nanomed Nano Biol Med. 2005;1:10-21.

Dalby M, Riehle M, Johnstone H, Affrossman S, Curtis A. Investigating the limits of filopodial sensing: A brief report using SEM to image the interaction between 10 nm high nano-topography and fibroblast filopodia. Cell Biol Int. 2004;28:229-36.

Poinern GEJ, Shackleton R, Mamun SI, Fawcett D. Significance of novel bioinorganic anodic aluminium oxide nanoscaffolds for promoting cellular response. Nanotechnol Sci Appl. 2010;3:1-14.

Le X, Poinern GEJ, Ali N, Berry C, Fawcett D. Engineering a biocompatible scaffold with either micrometre or nanometre scale surface topography for promoting protein adsorption and cellular response. Int J Biomat. 2013; Article ID 782549:16 pages.

Karlsson M, Palsgard E, Wilshaw PR, Di Silvio L. Initial in vitro interaction of osteoblasts with nano-porous alumina. Biomaterials. 2003;24:3039-46.

Nguyen K, Shukla K, Moctezuma M, Tang L. Cellular and molecular responses of smooth muscle cells to surface nanotopography. J Nanosci Nanotechnol. 2007;7:2823-2.

Virology Laboratory procedure VIW-17, Animal Health Laboratories, Animal Virology, Department of Agriculture and Food, 3 Baron Hay Court, Kensington, Western Australia 6151, Australia.

Whatman® Anopore: Available at www.whatman.com/products, Accessed November 2014.

Poinern GEJ, Le X, Loomes C, Fawcett D. Biocompatibility of composite membranes composed of anodic aluminium oxide (AAO) and Poly (2-hydroxyethylmethacrylate) for use as a cell culture substrates. Materials Letters. 2014;131:182-5.

Poinern GEJ, Le X, O’Dea M, Becker T, Fawcett D. Biocompatibility of synthesised nano-porous anodic aluminium oxide membranes for use as a cell culture substrate for the Vero cell line: A preliminary study. Biomed Research International. 2014;1:1-11.

Schafer C, Borm B, Born S, Mohr C, Eibl EM, Hoffman B. One step ahead: Role of filopodia in adhesion formation during cell migration of Keratinocytes. Experimental Cell Research. 2009;315:1212-24.