A study on the pattern of antibiotic resistance on different infective organisms in a tertiary care hospital
DOI:
https://doi.org/10.18203/2320-6012.ijrms20253595Keywords:
Antibiotic resistance, Escherichia coli, Tertiary Care Hospital, Empirical therapy, Antimicrobial susceptibilityAbstract
Background: A serious problem for global health, antibiotic resistance raises morbidity, mortality, and medical expenses. A clear understanding of local resistance patterns is required to maximize empirical therapy and infection control. Objectives of the study was to identify infective organisms and determine their antibiotic resistance profiles in a tertiary care hospital. To assess the resistance patterns across various antibiotic classes.
Methods: A cross-sectional study was conducted in a tertiary care center. Clinical specimens (pus, urine, sputum, blood, and tissue) were obtained and processed using standard microbiological culture techniques. Isolates were identified, and antibiotic susceptibility testing was performed via the disc diffusion method in accordance with Clinical and Laboratory Standards Institute (CLSI) guidelines.
Results: As a result, the present data analysis releaving that the culture positive was seen more in the 46-65 age range and with a higher propotion of females. The majority of the isolated organisms were Escherichia coli (23%), Staphyloccus aureus (21%), Klebisella pneumonia (21%), Pseudomonas aeruginosa (11%). The sample that grew organisms were pus (65), urine (13), sputum (17), blood (3), tissue (2).
Conclusions: The prevalence of drug-resistant infections, particularly in patients aged 46–65 and among females, underscores the importance of routine antimicrobial surveillance. E. coli’s high cefuroxime resistance and maintained amikacin susceptibility highlight the need for evidence-based empirical therapy. Strengthening hospital-based antibiotic stewardship programs is recommended to curb the spread of resistance.
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References
Savanur SS, Gururaj H. Study of Antibiotic Sensitivity and Resistance Pattern of Bacterial Isolates in Intensive Care Unit Setup of a Tertiary Care Hospital. Indian J Crit Care Med. 2019;23(12):547-55. DOI: https://doi.org/10.5005/jp-journals-10071-23295
Samanipour A, Dashti-Khavidaki S, Abbasi MR, Abdollahi A. Antibiotic resistance patterns of microorganisms isolated from nephrology and kidney transplant wards of a referral academic hospital. J Res Pharm Pract. 2016;5(1):43-51. DOI: https://doi.org/10.4103/2279-042X.176559
Magiorakos AP, Srinivasan A, Carey RB, Carmeli Y, Falagas ME, Giske CG, et al. Multidrug-resistant, extensively drug-resistant and pandrug-resistant bacteria: an international expert proposal for interim standard definitions for acquired resistance. Clinical microbiology and infection. 2012;18(3):268-81. DOI: https://doi.org/10.1111/j.1469-0691.2011.03570.x
Anning AS, Baah E, Buabeng SD, Baiden BG, Aboagye B, Opoku YK, et al. Prevalence and antimicrobial resistance patterns of microbes isolated from individuals attending private diagnostic centre in Cape Coast Metropolis of Ghana. Scientific Reports. 2022;12(1):14282. DOI: https://doi.org/10.1038/s41598-022-18595-w
Mamuye Y. Antibiotic resistance patterns of common Gram-negative uropathogens in St. Paul’s Hospital Millennium Medical College. Ethiopian Journal of Health Sciences. 2016;26(2):93-100. DOI: https://doi.org/10.4314/ejhs.v26i2.2
Andayani N, Mahdani W, Nisyra M, Agustin H. Distribution and antibacterial susceptibility pattern of isolated bacteria from endotracheal aspirates among ventilator-assisted pneumonia patients in Indonesia. Narra J. 2023;3(1):e149. DOI: https://doi.org/10.52225/narra.v3i1.149
Chakraborty M, Sardar S, De R, Biswas M, Mascellino MT, Miele MC, et al. Current trends in antimicrobial resistance patterns in bacterial pathogens among adult and pediatric patients in the intensive care unit in a tertiary care hospital in Kolkata, India. Antibiotics. 2023;12(3):459. DOI: https://doi.org/10.3390/antibiotics12030459
Uc-Cachón AH, Gracida-Osorno C, Luna-Chi IG, Jiménez-Guillermo JG, Molina-Salinas GM. High prevalence of antimicrobial resistance among gram-negative isolated bacilli in intensive care units at a tertiary-care hospital in Yucatán Mexico. Medicina (Kaunas). 2019;55(9):588. DOI: https://doi.org/10.3390/medicina55090588
Garg VK, Mishra S, Gupta N, Garg R, Sachidanand B, Vinod K, Gautam H, Kapil A, Bhatnagar S. Microbial and antibiotic susceptibility profile among isolates of clinical samples of cancer patients admitted in the intensive care unit at regional tertiary care cancer center: a retrospective observational study. Indian J Crit Care Med. 2019;23(2):67-72. DOI: https://doi.org/10.5005/jp-journals-10071-23119
E. Abou Warda A, Molham F, Salem HF, Mostafa-Hedeab G, ALruwaili BF, Moharram AN, Sebak M, Sarhan RM. Emergence of High Antimicrobial Resistance among Critically Ill Patients with Hospital-Acquired Infections in a Tertiary Care Hospital. Medicina (Kaunas). 2022;58(11):1597. DOI: https://doi.org/10.3390/medicina58111597
Travasso C. India draws a red line under antibiotic misuse. BMJ. 2016 Feb 29;352:i1202. DOI: https://doi.org/10.1136/bmj.i1202
Global Antibiotic Resistance Partnership (GARP)-India Working Group. Rationalizing antibiotic use to limit antibiotic resistance in India. Indian J Med Res. 2011;134(3):281-94.
Frost I, Van Boeckel TP, Pires J, Craig J, Laxminarayan R. Global geographic trends in antimicrobial resistance: the role of international travel. J Travel Med. 2019 Dec 23;26(8):taz036. DOI: https://doi.org/10.1093/jtm/taz036
Saravanan R, Raveendaran V. Antimicrobial resistance pattern in a tertiary care hospital: An observational study. J Basic Clin Pharm. 2013;4(3):56-63. DOI: https://doi.org/10.4103/0976-0105.118797
Raghunath D. Emerging antibiotic resistance in bacteria with special reference to India. J Biosci. 2008;33(4):593-603. DOI: https://doi.org/10.1007/s12038-008-0077-9
Mithi B, Luhanga M, Kaminyoghe F, Chiumia F, Banda DL, Nyama L. Antibiotic use and resistance patterns at Rumphi District Hospital in Malawi: a cross-sectional study. BMC Infect Dis. 2024;24(1):445. DOI: https://doi.org/10.1186/s12879-024-09333-w
Moolchandani K, Sastry AS, Deepashree R, Sistla S, Harish BN, Mandal J. Antimicrobial resistance surveillance among intensive care units of a tertiary care hospital in Southern India. Journal of clinical and diagnostic research. Clin Diagn Res. 2017;11(2):DC01-DC07. DOI: https://doi.org/10.7860/JCDR/2017/23717.9247
Fatima T, Shahid MH, Khan AZ, Asif S, Ghumman AR, Afzal MF, Askri Z, Shahid MH. The Emergence of Multiple Antibiotic Resistance in Culture Sensitivities of Post-surgical Patients in Lahore General Hospital, Lahore. Cureus. 2022;14(3):e23212. DOI: https://doi.org/10.7759/cureus.23212
Zhang X, Gao H, Fu J, Lin F, Khaledi A. Overview on urinary tract infection, bacterial agents, and antibiotic resistance pattern in renal transplant recipients. J Res Med Sci. 2021;26:26. DOI: https://doi.org/10.4103/jrms.JRMS_286_18
Muto CA, Jernigan JA, Ostrowsky BE, Richet HM, Jarvis WR, Boyce JM, Farr BM. SHEA guideline for preventing nosocomial transmission of multidrug-resistant strains of Staphylococcus aureus and enterococcus. Infect Control Hosp Epidemiol. 2003;24(5):362-86. DOI: https://doi.org/10.1086/502924
Zhang C, Gong YL. Analysis of distribution and drug resistance of pathogens from the wounds of 1 310 thermal burn patients]. Zhonghua Shao Shang Za Zhi. 2018;34(11):802-8.
Magill SS, Edwards JR, Bamberg W, Beldavs ZG, Dumyati G, Kainer MA, et al. Emerging infections program healthcare-associated infections and antimicrobial use prevalence survey team. Multistate point-prevalence survey of health care-associated infections. N Engl J Med. 2014;370(13):1198-208. DOI: https://doi.org/10.1056/NEJMoa1306801
Lambert ML, Suetens C, Savey A, Palomar M, Hiesmayr M, Morales I, et al. Clinical outcomes of health-care-associated infections and antimicrobial resistance in patients admitted to European intensive-care units: a cohort study. Lancet Infect Dis. 2011;11(1):30-8. DOI: https://doi.org/10.1016/S1473-3099(10)70258-9
Depuydt PO, Blot SI. Antimicrobial resistance in nosocomial bloodstream infection associated with pneumonia and the value of systematic surveillance cultures in an adult intensive care unit. Crit Care Med. 2006;34(3):653-9. DOI: https://doi.org/10.1097/01.CCM.0000201405.16525.34
Kollef MH. Providing appropriate antimicrobial therapy in the intensive care unit: surveillance vs. de-escalation. Crit Care Med. 2006;34(3):903-5. DOI: https://doi.org/10.1097/01.CCM.0000202128.30405.60
Combes A, Luyt CE, Trouillet JL. Impact of antibiotic-resistant bacteria on the outcome of ventilator-associated pneumonia. Semin Respir Crit Care Med. 2006;27(1):23-8. DOI: https://doi.org/10.1055/s-2006-933670
World Health Organization. Global Action Plan on Antimicrobial Resistance. WHO; Geneva, Switzerland: 2015.
Khilnani GC, Zirpe K, Hadda V, Mehta Y, Madan K, Kulkarni A, et al. Guidelines for antibiotic prescription in intensive care unit. Indian J Crit Care Med. 2019;23(Suppl 1):S1-S63. DOI: https://doi.org/10.5005/jp-journals-10071-23101
Kulkarni AP, Sengar M, Chinnaswamy G, Hegde A, Rodrigues C, Soman R, et al. Indian antimicrobial prescription guidelines in critically ill immunocompromised patients. Indian J Crit Care Med. 2019;23(Suppl 1):S64-S96. DOI: https://doi.org/10.5005/jp-journals-10071-23102
Muteeb G, Rehman MT, Shahwan M, Aatif M. Origin of antibiotics and antibiotic resistance, and their impacts on drug development: A narrative review. Pharmaceuticals. 2023;16(11):1615. DOI: https://doi.org/10.3390/ph16111615
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