Association of ABO and Rh blood groups to HBV, HCV infections among blood donors in a blood bank of tertiary care teaching hospital in Southern India: A retrospective study

Sreedhar Babu KV, Suresh Bandi, Sindhuja Kondareddy, Anitha Madithadu


Background: ABO blood group has been found to be associated with the risk of several diseases. Infection with hepatitis B virus (HBV) and hepatitis C virus (HCV) are also the major health problems worldwide. This work was therefore aimed at assessing the ABO and Rh blood group antigens and its association with HBV and HCV seroreactive status among healthy blood donors.

Methods: This is a retrospective cross-sectional analytical study carried out in the department of Transfusion Medicine of a tertiary care teaching hospital blood bank for a period of 6 years (January 2009 to December 2014). Data retrieved from blood bank records included the donors’ ABO group, Rh type and the result of HBV, HCV serology.

Results: A total number of 41652 blood donors were registered and screened during the study period. The commonest blood group was O constituting 41.5% followed by B-32.6%, A-19.8%, AB-6.1% and Bombay-0.02%. Rh-D positive donors were 92.9% and remaining 7.1% were Rh-D negative. The overall prevalence of HBV and HCV were 2.4% and 0.4% respectively. Among total HBV and HCV seroreactive donors 41.7% and 37.9% were O blood group, B-30.9%, 32.7% A-21.6%, 21.2% and AB-5.7%, 8% respectively. Among the total HBV and HCV seroreactive group, 93.7% and 93.1% had Rh-D positive blood group and remaining 6.3% and 6.9% had Rh-D negative blood group respectively.

Conclusion: In this study conducted to determine the predominant blood group antigen and its association with HBV and HCV seroreactivity, there was no association between blood group antigens with these infections.



Blood donors, Seroprevalence,Hepatitis C, Hepatitis B surface antigen, ABO and Rh blood group

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Table of blood group systems. International Society of Blood Transfusion (ISBT). Available from:

Mollison PL. The genetic basis of Rh blood group system. Transfusion. 1994;34:539-41.

Skaik Y, El-Zyan N. Spectrum of ABO and Rh (D) blood groups amongst the Palestinian students at Al-Azhar University-Gaza. Pak J Med Sci. 2006;22:333-5.

Gerald L, Douglas M. Principles and practice of infectious disease. 5th ed. Churchill; 2000; 1-39.

Ahmad J, Taj AS, Rahim A, Shah A, Rehman M. Frequency of Hepatitis B and Hepatitis C in healthy blood donors of NWFP: a single center experience. J Postgrad Med Inst. 2004;18:343-52.

Quarterly Newsletter from the National Centre for Disease Control (NCDC) January-March 2014 Volume 3, Issue 1. Available from: http://ncdc.

Hepatitis B fact sheet number 204 (updated March 2015). World Health Organization. Available from: /en/.

Hepatitis C Fact sheet number 164 (Updated April 2014). World Health Organization, Available from: en/.

Malik V. Law relating to drugs and cosmetics. 2011, 22nd ed: Eastern Book Company Lucknow.

Stayboldt C, Rearden A, Lane TA. B antigen acquired by normal A1 red cells exposed to a patient's serum. Transfusion. 1987;27:41-4.

Matsushita S, Imamura T, Mizuta T, Hanada M. Acquired B antigen and polyagglutination in a patient with gastric cancer. The Japanese Journal of Surgery. 1983;13:540-2.

Kremer Hovinga I, Koopmans M, de Heer E, Bruijn J, Bajema I. Change in blood group in systemic lupus erythematosus. Lancet. 2007;369:186-7.

Chown B, Lewis M, Kaita K. A new Kell blood-group phenotype. Nature. 1957;180: 711.

Miller LH, Mason SJ, Clyde DF, McGinniss MH. The resistance factor to Plasmodium vivax in blacks. The Duffy-blood-group genotype, FyFy. N Engl J Med. 1976;295:302-4.

Kwiatkowski DP. How Malaria Has Affected the Human Genome and What Human Genetics Can Teach Us about Malaria. Am J Hum Genet. 2005; 77:171-92.

Das S, Kumar MLH. Association of blood group types to hepatitis B and hepatitis C virus infection among blood donors: a five years institutional based study. Int J Basic Appl Med Sci. 2012;2:191-5.

Behal R, Jain R, Behal KK, Bhagoliwal A, Aggarwal N, Dhole TN. Seroprevalence and risk factors for hepatitis B virus infection among general population in Northern India. Arq Gastroenterol. 2008;45:137-40.

Jeremiah ZA, Koate B, Buseri F, Emelike F. Prevalence of antibodies to hepatitis C virus in apparently healthy Port Harcourt blood donors and association with blood groups and other risk indicators. Blood Transfus. 2008;6:150-5.

Omar AAA, Al-Hayan NN, Mohammed MJ. The infection with HBV and HCV and their relationship to ABO blood group among blood donors. J Fac Med Baghdad. 2012; 54:52-5.

Tyagi S, Tyagi A. Possible correlation of transfusion transmitted diseases with Rh type and ABO blood group system. JCDR. 2013;7:1930-1.

Kumar MR, Rao MS, Pulicherla KK, Ghosh M, Kumar HM, Rekha VPB. Studies on the distribution of hepatitis B (HBV) and human immunodeficiency virus (HIV): their relation to blood groups and rhesus (Rh) factor in Guntur district of Andhra Pradesh, India. Asian J Pharm Clin Res. 2013;6:109-11.

Nigam JS, Singh S, Kaur V, Giri S, Kaushal RP. The prevalence of transfusion transmitted infections in ABO blood groups and Rh type system. Hematology Reports. 2014;6:5602.

Anwar MS, Siddiqi GM, Haq S, Khokhar NG, Jaffery G. Association of blood group types to hepatitis B and hepatitis C virus infection. Biomedica 2011;1:57-61.

Mohammadali F, Pourfathollah A. Association of ABO and Rh blood groups to blood-borne infections among blood donors in Tehran–Iran. Iranian J Publ Health. 2014;43(7):981-9.

Moulds JM, Nowicki S, Moulds JJ, Nowicki BJ. Human blood groups: incidental receptors for viruses and bacteria. Transfusion. 1996;36:362-74.

Moulds, Joann M. Blood group associations with parasites, bacteria, and viruses. Transfusion Medicine Reviews. 2000;14:302-11.