Microbiological profile of central line-associated bloodstream infections at a tertiary care facility
DOI:
https://doi.org/10.18203/2320-6012.ijrms20253612Keywords:
CLABSI, ICU, Antibiotic susceptibility testingAbstract
Background: High-risk and critically ill patients admitted to the ICU often require catheter insertion for administering fluids, blood products, medications, and performing hemodynamic monitoring. Healthcare-associated infections (HAIs) are common in the ICU due to prolonged hospital stays. Central line-associated bloodstream infections (CLABSI) are significant healthcare-associated infections that necessitate surveillance to decrease their occurrence. This study aims to identify the etiological agents and AST patterns of CLABSI in the Medicine ICU.
Methods: A hospital-based surveillance study was conducted from January to December 2023 in the medicine ICU. Blood samples were collected from suspected patients, processed, organisms isolated, and their antibiotic susceptibility tested following our department’s SOPs. CLABSI was diagnosed as per the AIIMS guidelines. CLABSI rates were calculated monthly as part of an HIC surveillance. IPC measures were implemented to reduce the CLABSI rate under surveillance.
Results: Out of 628 blood samples received from the medicine ICU, 178 (28.3%) were positive by culture, and 450 were sterile. Of the 178 positive samples, 65 (10.35%) cases were HAI BSI, with 19 (3.02%) being CLABSI HAI and 46 (7.32%) non-CLABSI HAI. Among CLABSI HAI, Gram-negative bacteria (GNBs) are the most common organisms, accounting for 68.4% of isolates, while Gram-positive bacteria (GPBs) contribute 31.6%.
Conclusions: Excessive use of invasive procedures increases the risk of MICU patients becoming colonized with highly resistant bacteria. Identifying the range of bacteria causing BSI helps clinicians apply proper management strategies and antibiotic policies to improve patient outcomes.
Metrics
References
Bhatawadekar SM, Arunima , Lahiri KK. Bacterial profile of central line associated blood stream infections in I.C.U patients from tertiary care hospital. IP Int J Med Microbiol Trop Dis. 2025;4(1):31-5. DOI: https://doi.org/10.18231/2455-6807.2018.0007
Franklin D. COVID-19 vaccination coverage, and rates of SARS-CoV-2 infection and COVID-19–associated hospitalization among residents in nursing homes—National Healthcare Safety Network, United States, October 2023–February 2024. MMWR. Morbidity and Mortality Weekly Report. 2024;73. DOI: https://doi.org/10.15585/mmwr.mm7315a3
Tabaseera N, Hanumantha S. Catheter associated blood stream infection (CLABSI)-bacteriological profile and antibiotic susceptibility pattern in nnicu of a tertiary care hospital in Andhra Pradesh, India. Int J Curr Microbiol Appl Sci. 2018;7:3935-41. DOI: https://doi.org/10.20546/ijcmas.2018.707.457
Patel R, Jain MR. Bacteriological profile and antibiotic sensitivity pattern of isolates from blood culture in suspected septicemic patients attending tertiary care hospital. IP Int J Med Microbiol Trop Dis. 2025;5(4):198-203. DOI: https://doi.org/10.18231/j.ijmmtd.2019.045
Chawda H, Mistry M, Parmar T. A study of microbiological profile and antibiotic susceptibility pattern of the organisms isolated from blood stream infection in patients of critical care units in tertiary care hospital in Western India. IP Int J Med Microbiol Trop Dis. 2025;5(2):83-6. DOI: https://doi.org/10.18231/j.ijmmtd.2019.017
Gill MK, Sharma S. Bacteriological profile and antibiotic resistance pattern in blood stream infection in critical care units of a tertiary care hospital in North India. Indian J Microbiol Res. 2025;3(3):270-4. DOI: https://doi.org/10.5958/2394-5478.2016.00059.5
Gohel K, Jojera A, Soni S, Gang S, Sabnis R, Desai M. Bacteriological profile and drug resistance patterns of blood culture isolates in a tertiary care nephrourology teaching institute. Biomed Res Int. 2014;4:153747. DOI: https://doi.org/10.1155/2014/153747
Darji SM, Patel N. Central line associated blood stream infection: microbiological profile and its antimicrobial susceptibility pattern at tertiary care centre. J Pure Appl Microbiol. 2023;17(2):911-8. DOI: https://doi.org/10.22207/JPAM.17.2.18
Lin WP, Chang YC, Wu UI, Hung MC, Chuang PY, Wang JT, et al. Multimodal interventions for bundle implementation to decrease central line-associated bloodstream infections in adult intensive care units in a teaching hospital in Taiwan, 2009-2013. J Microbiol Immunol Infect. 2018;51(5):644-51. DOI: https://doi.org/10.1016/j.jmii.2017.08.008
Bhatnagar R, Patel P. Study of antimicrobial resistance pattern in blood isolates from critical care unit at a Tertiary Care hospital, Udaipur, Rajasthan. Indian J Microbiol Res. 2025;5(1):61-5. DOI: https://doi.org/10.18231/2394-5478.2018.0012
Khodare A, Kale P, Pindi G, Joy L, Khillan V. Incidence, microbiological profile and impact of preventive measures on central line-associated bloodstream infection in liver care intensive care unit. Indian J Crit Care Med. 2020;24(1):17-22. DOI: https://doi.org/10.5005/jp-journals-10071-23325
Wasi S, Daga MK, Mawari G, Kumar N, Sharma U, Hussain M, et al. Current Pattern and Clinico-Bacteriological Profile of Healthcare Associated Infections in an ICU Setting: A Study from a Tertiary Care Centre in Delhi. J Adv Res Med. 2022;9(1):1-10. DOI: https://doi.org/10.24321/2349.7181.202201
Marcos M, Soriano A, Iñurrieta A, Martínez JA, Romero A, Cobos N, et al. Changing epidemiology of central venous catheter-related bloodstream infections: increasing prevalence of Gram-negative pathogens. J Antimicrob Chemother. 2011;66(9):2119-25. DOI: https://doi.org/10.1093/jac/dkr231
Ujesh SN, Jayaprada R, Ramakrishna N, Sharma KK, Rao MH, Samantaray A. A study of microbiological profile and its antimicrobial susceptibility patterns related to central line-associated bloodstream infections in respiratory intensive care unit in a tertiary care hospital. J Clin Sci Res. 2020;9(1):25-30. DOI: https://doi.org/10.4103/JCSR.JCSR_18_19
Downloads
Published
How to Cite
Issue
Section
