DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20150319

Comparative study of oxidative stress in individuals with and without age related hearing loss

Vineeth VK, Damodara Gowda KM

Abstract


Background: Age related hearing loss is an universal feature of mammalian ageing and refers to sensorineural hearing impairment in elderly individuals. It was reported that increased generation of free radicals during cellular metabolism plays a major role in age related disorders. Therefore, the occurrence of age related hearing loss depends mainly on antioxidant status of the body. Hence, this study was undertaken to investigate the status of Glutathione and Superoxide dismutase in age related hearing loss.

Methods: This study was conducted after the approval from institutional ethical committee. The study group included 25 patients diagnosed for age related hearing loss between the age group of 55-80 years of either sex and 25 healthy age and sex matched individuals as controls. Estimation of Glutathione was done by DTNB method and Superoxide Dismutaseusing standard procedures. The data was analysed using One Way ANOVA. P value less than 0.05 will be considered the level of significance.

Results: The results showed that, the Glutathione level in Hearing Loss patients was increased significantly (p=0.0001) as compared to normal controls. The SOD Activity has declined significantly (p=0.001) in Hearing Loss patients as compared to normal controls.

Conclusion: We found an association between the level of Glutathione and Super Oxide Dismutase in age related hearing loss. Thus the serum Glutathione and Super Oxide Dismutase level can be used as a biomarker for the assessment of age related hearing loss.

 


Keywords


Presbycusis, Glutathione, Super Oxide Dismutase, DTNB method

Full Text:

PDF

References


Houston DK, Johnson MA, et al. Age related hearing loss, vitamin B12 and folate in elderly women. Am J Clin Nutr. 1999;69:564-71.

Kawamoto K, Su-Hua S, et.al. Antioxidant Gene Therapy can Protect Hearing and Hair cells from Ototoxicity. Molecular Therapy. 9(2):648.

Ilhami Yildirim, Metin Kilinc et.al. The effects of noise on hearing and oxidative stress in Textile workers. Industrial Health 2007,45,743-749.

Adiga U, Adiga S. Total Antioxidant Activity in old age. Biomed Res. 2008;19(3):185-6.

McFadden SL, Ohlemiller, Ding D, Shero M, Salvi RJ. The influnce of superoxide dismutase and glutathione peroxidase deficiencies on noise induced hearing loss in mice. Noise Health. 2001;3:49-64.

Donald E Coling, Kenneth C Y et.al. Effect of SOD1 Over expression on Age and Noise Related Hearing Loss. Free radical Biology and Medicine. 2003;34(7):873-80.

Coling D, Chen SC, Chi LH, Daniel SJ, Henderson D. Age realated changes in antioxidant enzymes related to hydrogen peroxide in inner ear. Neurosci Lett. 2009;464(1):22-5.

Nabatchian F, Khaghani S, Bagherian R, Miri R, Mahmoodi M, Pasalar P. The relationship between oxidative stress and onset of coronary artery disease. Medical Journal of the Islamic Republic of Iran. 2004;18(2):101-5.

Pompella A, Visvikis A, Paolicchi A, De Tata V, Casini AF. The changing faces of glutathione, a cellular protagonist. Biochem Pharmacol. 2003;66(8):1499–503.

Borgstahl GE, Parge HE, Hickey MJ, Johnson MJ, Boissinot M, Hallewell RA, et al. Human mitochondrial manganese superoxide dismutase polymorphic variant Ile58Thr reduces activity by destabilizing the tetrameric interface. Biochemistry. 1996;35(14):4287–97.

Sharma SM, Mohan M, Kumari S, Sorake SJ. Evaluation of Glutathione in oral squamous cell carcinoma. Journal of Maxillofacial Oral Surgery. 2003;8(3):270-4.

Beaurchamp C, Fridovich, I. Superoxide dismutase improved assay applicable to acrylamide gels. Analytical biochemistry. 1971;44:276-87.

Someya S, Prolla TA. Mitochondrial oxidative damage and apoptosis in age-related hearing loss. Mechanisms of Ageing and Development. 2010;131(7-8):480–6.

Seidman MD, Khan MJ, Dolan DF, Quirk WS. Age related differences in cochlear microcirculation and auditory brain stem response. Arch Otolaryngol Head Neck Surg. 1996;122:1221-6.

Seidman MD, Ahmad N, Joshi D, Seidman J, Thawani S, Wayne S. Quirk. Age-related Hearing Loss and its Association with Reactive Oxygen Species and Mitochondrial DNA damage. Acta Otolaryngol. 2004;552:16-24.