Anticandidal efficacy of Shikakai and rose petals in herbal denture cleanser formulation: an in vitro study

Sanjana A.; Sinchana K.; Mounashree U.; Nanditha B. R.

doi:10.18203/2320-6012.ijrms20263094

Authors

Sanjana A. Department of Pharmaceutics, Rajeev College of Pharmacy, Hassan, Karnataka, India
Sinchana K. Department of Pharmaceutical Analysis, People’s Education Society University, Bengaluru, Karnataka, India
Mounashree U. Department of Pharmacology, Rajeev College of Pharmacy, Hassan, Karnataka, India
Nanditha B. R. Department of Pharmaceutical Chemistry, Rajeev College of Pharmacy, Hassan, Karnataka, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20263094

Keywords:

Herbal denture cleanser, Anticandidal activity, Effervescent tablet, Acacia concinna, Candida albicans

Abstract

Background: Chemical denture cleansers may cause irritation, discoloration, and allergic reactions in denture wearers. Herbal agents with antimicrobial properties are being explored as safer alternatives to prevent denture-associated infections such as oral candidiasis. This study aimed to develop and evaluate a natural denture cleanser using Acacia concinna (Shikakai) and Rosa spp. (rose petals).

Methods: Dried plant materials were extracted using the Soxhlet extraction method and subjected to qualitative phytochemical screening. Antimicrobial activity against Candida species was assessed in vitro. The extracts were formulated into effervescent tablets for immersion-type denture cleansing. The tablets were evaluated for weight variation, hardness, friability, pH, disintegration time, and in vitro dissolution. Cleaning efficiency and anticandidal activity were also studied.

Results: Phytochemical screening confirmed the presence of flavonoids, saponins, tannins, and glycosides, with Shikakai extract showing a richer phytochemical profile. Antimicrobial studies demonstrated stronger antifungal activity of Shikakai against Candida species compared to rose petal extract. The formulated tablets met acceptable quality control parameters. In vitro dissolution studies showed about 20% release within one hour and nearly 90% release within six hours. Shikakai-based formulations exhibited superior cleaning efficiency and anticandidal activity.

Conclusions: Shikakai-based herbal denture cleanser tablets showed effective antifungal activity, satisfactory tablet properties, and good cleaning performance, indicating their potential as a natural alternative to synthetic denture cleansers. Further clinical studies are needed to confirm long-term safety and user acceptability.

References

Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014;4:177.

Washington Smiles Complete Health Dentistry. Types of dentures explained and what is best for you. Washington Smiles. Missouri. Available at: https://www.washmosmiles.com/types-of-dentures-explained/. Accessed on 14 June 2026.

Dr. Nechupadam Dental Clinic. Parts of complete denture. Nechupadam Dental. Available at: https://www.nechupadamdental.com. Accessed on 14 June 2026.

Owens. Classification and elements of removable partial dentures. My Perfect Smile DDS. Available at: https://www.myperfectsmiledds.com. Accessed on 14 June 2026.

Andreescu CF, Ghergic DL, Botoaca O, Violeta H, Banateanu AM, Patroi D. Evaluation of different materials used for fabrication of complete digital denture. Mater Plast. 2018;55(1):124-8.

Fueki K, Ohkubo C, Yatabe M, Ichiro A, Masahiro A, Satoshi I, et al. Clinical application of removable partial dentures using thermoplastic resin-Part I: Definition and indication of non-metal clasp dentures. J Prosthodont Res. 2014;58(1):3-10.

Sharma AHSS, Shashidhara HS. A review: Flexible removable partial dentures. J Dent Med Sci. 2014;13(12):58-62.

Tandon R, Gupta S, Agarwal SK. Denture base materials: From past to future. Indian J Dent Sci. 2010;2(2):33-9.

Machado Dental Center. What are the different materials options to make dentures? Machado Dental Center. Available at: https://www.machadodentalcenter.com. Accessed on 14 June 2026.

MyDentureCare. Different types of denture cleansers. MyDentureCare. Available at: https://www.mydenturecare.com. Accessed on 14 June 2026.

De Castro ML, García-Ayuso LE. Soxhlet extraction of solid materials: An outdated technique with a promising innovative future. Anal Chim Acta. 1998;369(1-2):1-10.

ScienceDirect Topics. Soxhlet extraction-an overview. ScienceDirect. Available at: https://www.sciencedirect.com. Accessed on 14 June 2026.

Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: An overview. Int J Chem Stud. 2020;8(2):603-8.

Pandey A, Tripathi S. Concept of standardization, extraction and pre-phytochemical screening strategies for herbal drug. J Pharmacogn Phytochem. 2014;2(5):10.

Ayoola GA, Coker HA, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC, et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in Southwestern Nigeria. Trop J Pharm Res. 2008;7(3):1019-24.

Pharmapproach. Manufacture of tablets by dry granulation method. Pharmapproach. Available at: https://www.pharmapproach.com. Accessed on 14 June 2026.

Patel DM, Patel RJ. Formulation and evaluation of effervescent tablets: A review. ResearchGate. 2025.

Ekor M. The growing use of herbal medicines: issues relating to adverse reactions and challenges in monitoring safety. Front Pharmacol. 2014;4:177.

Shaikh JR, Patil MK. Qualitative tests for preliminary phytochemical screening: an overview. Int J Chem Stud 2020;8(2):603-8.

Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev. 1999;12(4):564-82.

Daglia M. Polyphenols as antimicrobial agents. Curr Opin Biotechnol. 2012;23(2):174-81.

Parekh J, Chanda S. Antibacterial and antifungal activity of Acacia concinna. Afr J Biotechnol. 2007;6(5):582-5.

Sahu MC, et al. Phytochemical analysis and antimicrobial activity of Acacia concinna. Int J Pharm Sci Rev Res. 2013;21(2):215-8.

Patel RM, Patel MM. Formulation and evaluation of effervescent tablets: a review. Int J Pharm Sci Rev Res. 2014;24(2):171-6.

Allen LV. Pharmaceutical dosage forms and drug delivery systems. 10th ed. Philadelphia: Lippincott Williams and Wilkins. 2013.

Ramage G, Kelley T, Wickes BL, López-Ribot JL, Redding SW. Denture stomatitis: a role for Candida biofilms. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2004;98(1):53-9.

Nikawa H, et al. In vitro evaluation of antifungal effect of denture cleansers on Candida albicans. J Oral Rehabil. 1999;26(6):484-9.

Pradeep AR, et al. Clinical evaluation of herbal dentifrice in comparison with conventional dentifrice. J Clin Periodontol. 2012;39(3):222-7.