Assessment of cryogenic safety practices, exposure and occupational hazards in embryology laboratories of IVF clinics in Abuja, Nigeria

Kayode Adisa; Henry O. Sawyerr; Ifeoma L. Akunwa; Mutiat Salawu

doi:10.18203/2320-6012.ijrms20261657

Authors

Kayode Adisa Department of Andrology and Embryology, Primecare Fertility Clinic Ltd, Garki, FCT, Abuja, Nigeria
Henry O. Sawyerr Department of Environmental Health Sciences, Ahmadu Bello University (ABU), Zaria, Kaduna, Nigeria
Ifeoma L. Akunwa Department of Environmental Health and Sanitation, Ministry of Environment, Federal Capital Territory (FCT), Nigeria
Mutiat Salawu Department of Agribusiness Management, Federal College of Animal Health and Production Health, Ibadan, Oyo State, Nigeria

DOI:

https://doi.org/10.18203/2320-6012.ijrms20261657

Keywords:

Assisted reproductive technology, Cryogenic safety, Embryology laboratory, Occupational hazards, Safety compliance

Abstract

Background: Assisted reproductive technology (ART) laboratories have cryogenic storage, which allows the long-term storage of gametes and embryos using liquid nitrogen in place of short-term storage. Although it is essential, cryogenic operations are known to create serious occupational hazards to the laboratory staff where there are no adequate safety measures. The study evaluated cryogenic safety practices, pattern of exposure, occupational hazards and compliance pattern among embryology laboratory staff in Abuja, Nigeria in vitro fertilizations (IVF) clinics.

Methods: A cross-sectional study of embryology lab staff in Abuja in selected IVF clinics was done in a descriptive study. The respondents filled a self-administered, structured questionnaire, which included data on cryogenic safety practices, exposure to cryogenic hazards and history of accidents. Frequencies and percentages were summarised using descriptive statistics. Principal component analysis (PCA) was used to identify underlying dimensions of cryogenic safety compliance across measured safety practices.

Results: The majority (90.24%) of respondents were directly involved in cryogenic storage, handling or supervision. 58.54% reported direct exposure to cryogenic liquids and 34.15% reported cryogenic related laboratory accidents. Principal component analysis identified two distinct dimensions of cryogenic safety compliance, reflecting procedural-behavioral practices and equipment monitoring activities.

Conclusions: High levels of staff exposure to cryogenic systems coexist with inconsistent engineering controls and uneven adherence to safety practices. The multidimensional compliance structure identified highlights the need for cryogenic safety management strategies that address both behavioral–procedural compliance and equipment monitoring practices in ART laboratories.

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References

Schiewe MC, Freeman M, Whitney JB, VerMilyea MD, Jones A, Aguirre M, et al. Comprehensive assessment of cryogenic storage risk and quality management concerns: Best practice guidelines for ART labs. J Assist Reprod Genet. 2019; 36(1):5-14. DOI: https://doi.org/10.1007/s10815-018-1310-6

Lin B, Gu H, Ni H, Guan B, Li Z, Han D, et al. Effect of mixing methane, ethane, propane and ethylene on the soot particle size distribution in a premixed propene flame. Combust Flame. 2018;193:54-60. DOI: https://doi.org/10.1016/j.combustflame.2018.03.002

CO2Meter. Liquid nitrogen safety requirements, 2025. Available at: https://www.co2meter.com/blogs/news/liquid-nitrogen-safety-requirements-osha. Accessed 01 January 2026.

National Institutes of Health. Protocol for use and maintenance of oxygen monitoring devices, 2023. Available at: https://ors.od.nih.gov/sr/dohs/Documents/protocol-for-use-and-maintenance-of-oxygen-monitoring-devices.pdf. Accessed 01 January 2026.

Murphy A, Lapczynski MS, Proctor G, Glynn TR, Domar AD, Gameiro S, et al. Comparison of embryologist stress, somatization, and burnout reported by embryologists working in UK HFEA-licensed ART/IVF clinics and USA ART/IVF clinics. Hum Reprod. 2024;39(10):2297-304. DOI: https://doi.org/10.1093/humrep/deae191

Priddle H, Pickup T, Palmer GA. Occupational health issues experienced by UK embryologists. Hum Fertil. 2022;25(4):753-62. DOI: https://doi.org/10.1080/14647273.2021.1871782

American Society for Reproductive Medicine. Cryostorage of reproductive tissues in the in vitro fertilization laboratory: A committee opinion. Fertil Steril. 2020;114(3):486-91. DOI: https://doi.org/10.1016/j.fertnstert.2020.06.019

Khan HL, Boothroyd C, Chang TA, Novero V, Chan DYL, Chen CH, et al. ASPIRE guidelines for assisted reproductive technology (ART) laboratory practice in low and medium resource settings. Fertil Reprod. 2023;5(3):115-33. DOI: https://doi.org/10.1142/S2661318223500184

Del Fante Z, Di Fazio N, Papale A, Tomao P, Del Duca F, Frati P, et al. Evaluation of physical risk during necropsy and morgue activities as risk management strategy. Int J Environ Res Publ Heal. 2021;18(16):8266. DOI: https://doi.org/10.3390/ijerph18168266

Maehira Y, Spencer RC. Harmonization of biosafety and biosecurity standards for high-containment facilities in low- and middle-income countries: An approach from the perspective of occupational safety and health. Front Publ Heal. 2019;7:249. DOI: https://doi.org/10.3389/fpubh.2019.00249

Klebanoff LE, Pratt JW, LaFleur CB. Comparison of the safety-related physical and combustion properties of liquid hydrogen and liquid natural gas in the context of the SF-BREEZE high-speed fuel-cell ferry. Int J Hydrog Energ. 2017;42:757-74. DOI: https://doi.org/10.1016/j.ijhydene.2016.11.024

Peterson TJ, Weisend JG II. Cryogenic safety: A guide to best practice in the lab and workplace. Springer Nature; 2019. DOI: https://doi.org/10.1007/978-3-030-16508-6

Scurlock RG. The future with cryogenic fluid dynamics. Phys Procedia. 2015;67:20-6. DOI: https://doi.org/10.1016/j.phpro.2015.06.005

PureAire Monitoring. Storing liquid nitrogen in laboratories: Which safety precautions should you take?, 2025. Available at: https://www.pureairemonitoring.com/additive_manufacuring_o2_analyzer-11/. Accessed 01 January 2026.

Mocellin P, Vianello C, Maschio G. Facing emerging risks in carbon sequestration networks. a comprehensive source modeling approach. Chem Engineer Transact. 2018;67:295-300.

Carboni M, Pio G, Mocellin P, Vianello C, Maschio G, Salzano E. Accidental release in the bunkering of LNG: Phenomenological aspects and safety zone. Ocean Eng. 2022;252:111163. DOI: https://doi.org/10.1016/j.oceaneng.2022.111163

Mocellin P, Pio G, Carboni M, Pilo F, Vianello C, Salzano E. On the effectiveness of mitigation strategies for cryogenic applications. J Loss Prev Process Ind. 2023;84:105123. DOI: https://doi.org/10.1016/j.jlp.2023.105123

Haier Biomedical. Safety considerations in a liquid nitrogen cryo preservation room, 2023. Available at: https://www.haiermedical.com/Safety-Considerations-in-A-Liquid-Nitrogen-Cryo-Preservation-Room.html. Accessed 01 January 2026.

Kim I, Kim H, Chang D, Jung DH, Sung HG, Park SK, et al. Emergency evacuation simulation of a floating LNG bunkering terminal considering the interaction between evacuees and CFD data. Saf Sci. 2021;140:105297. DOI: https://doi.org/10.1016/j.ssci.2021.105297

Pio G, Salzano E. Accidental combustion phenomena at cryogenic conditions. Safety. 2021;7(4):67. DOI: https://doi.org/10.3390/safety7040067

Pillai SP, Bradberry S, Newcomer M, Pittas T, Mathern K. A framework for personal protective equipment use in laboratories: Regulatory compliance and employee protection. Front Publ Heal. 2025;13:1586491. DOI: https://doi.org/10.3389/fpubh.2025.1586491