Bivariate and multivariate regression analysis of pesticide exposure duration, protective practices and PON1 (192Q/R) polymorphism among agricultural workers in Malerkotla and Rupnagar Districts of Punjab, India
DOI:
https://doi.org/10.18203/2320-6012.ijrms20262183Keywords:
192Q/R. Gene–environment interaction, Agricultural workers, PON1 polymorphism, Multivariate logistic regression, Occupational health, Personal protective equipment, Pesticide exposureAbstract
Background: Occupational pesticide exposure poses a significant public health risk among agricultural workers, particularly in developing regions with limited safety practices. Genetic variability, especially in the paraoxonase 1 (PON1) gene, influences individual susceptibility to organophosphate toxicity. The PON1 (192Q/R) polymorphism affects enzyme activity involved in pesticide detoxification.
Methods: A cross-sectional study was conducted at RIMT university hospital among 200 pesticide-exposed agricultural workers from the Malerkotla and Rupnagar districts of Punjab, India. Participants (184 males, 16 females) were selected based on occupational exposure. Exposure characteristics and safety practices were assessed using structured questionnaires. Genotyping of the PON1 (192Q/R) polymorphism was performed using PCR-RFLP. Bivariate logistic regression was used to examine associations between genotype and gender, while multivariate logistic regression evaluated relationships between exposure variables using combined genotype (QR+RR vs QQ) and allele (R vs Q) models. Adjusted odds ratios (ORs) with 95% confidence intervals (CIs) were calculated.
Results: No significant association was observed between PON1 polymorphism and gender. The QR genotype showed higher odds of occurrence in females (OR=3.61; p=0.28), whereas the RR genotype showed reduced odds (OR=0.70; p=0.52). The R allele was not significantly associated with gender (OR=0.75; p=0.59). Multivariate analysis indicated a gradual increase in the odds of R allele-containing genotypes with longer pesticide exposure, with workers exposed for >40 years showing higher odds (adjusted OR≈2.08), though not statistically significant (p=0.08). Protective measures were associated with reduced odds but lacked significance.
Conclusions: PON1 (192Q/R) polymorphism was not associated with gender; however, prolonged pesticide exposure showed a non-significant trend toward increased R allele prevalence, highlighting the need for improved occupational safety practices.
References
Romero Damalas CA, Koutroubas SD. Farmers’ exposure to pesticides: Toxicity types and ways of prevention. Toxics. 2016;4(1):19.
Kim KH, Kabir E, Jahan SA. Exposure to pesticides and the associated human health effects. Sci Total Environ. 2017;575:525–35.
Costa LG, Giordano G, Cole TB, Marsillach J, Furlong CE. Paraoxonase 1 (PON1) as a genetic determinant of susceptibility to organophosphate toxicity. Toxicology. 2013;307:115–22.
Devika D, Aulakh RK, Sharma R, Rani N. Analysis of pesticide and heavy metal bioaccumulation in earthworms from Central plain zone of Punjab. 2024.
Remoundou K, Brennan M, Sacchettini G, Panzone L. Factors affecting pesticide exposure in agriculture: A review of the literature. Int J Environ Res Public Health. 2014;11(7):6952–89.
Furlong CE, Holland N, Richter RJ, Bradman A, Ho A, Eskenazi B. PON1 status of farmworker mothers and children as a predictor of organophosphate sensitivity. Pharmacogenet Genomics. 2006;16(3):183–90.
Lee BW, London L, Paulauskis J, Myers J, Christiani DC. Association between human paraoxonase gene polymorphism and chronic symptoms in pesticide-exposed workers. J Occup Environ Med. 2003;45(2):118–22.
Muñoz-Quezada MT, Lucero BA, Barr DB, Steenland K, Levy K, Ryan PB, et al. Neurodevelopmental effects in children associated with exposure to organophosphate pesticides: A systematic review. Neurotoxicology. 2013;39:158–68.
Sambrook J, Fritsch EF, Maniatis T. Molecular cloning: A laboratory manual. 2nd ed. Cold Spring Harbor: Cold Spring Harbor Laboratory Press. 1989.
Gupta A, Saluja A, Saraswathy KN, Imnameren L, Yadav S, Dhamija RK. PON1 (Paraoxonase 1) Q192R gene polymorphism in ischemic stroke among North Indian population. Ann Indian Acad Neurol. 2022;25(1):100–105.
Mitra S, Kshatriya GK. Genetic variation at Q192R and L55M polymorphisms in paraoxonase-1 (PON1) among Indian populations. Environ Toxicol Pharmacol. 2016;45:182–90.
Jaiswar SP, Priyadarshini A, Singh A, Ahmad MK, Deo S, Sankhwar P. Association between PON1 (L55M and Q192R) genetic polymorphisms and recurrent pregnancy loss in North Indian women exposed to pesticides. Rev Bras Ginecol Obstet. 2021;43(11):805–10.
Srídon A, Ruchiwit K, Suwannahong K, Pongstaporn W, Sudjaroen Y. PON1 Q192R gene polymorphism and pesticide exposure status of rice farmers. Indian J Forensic Med Toxicol. 2020;14(4):3051–7.
Costa LG, Richter RJ, Li WF, Cole T, Guizzetti M, Furlong CE. Paraoxonase (PON1) as a biomarker of susceptibility for organophosphate toxicity. Biomarkers. 2003;8(1):1–12.
Costa LG, Furlong CE. Paraoxonase (PON1) in health and disease: Basic and clinical aspects. Crit Rev Toxicol. 2012;42(9):633–53.
Andersen HR, Wohlfahrt-Veje C, Dalgård C, Christiansen L, Main KM, Nellemann C, et al. Paraoxonase 1 polymorphism and prenatal pesticide exposure associated with adverse cardiovascular risk profiles at school age. PLoS One. 2012;7(5):36830.
Singh S, Kumar V, Thakur S, Banerjee BD. DNA damage and oxidative stress in pesticide-exposed workers: Role of genetic polymorphisms. Toxicol Appl Pharmacol. 2011;252(2):114–22.
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