Decoding Candida-speed and accuracy in species identification: a study from a tertiary care hospital in Western Vidarbha

Rajesh Dawre; Nazneen I. Malak; Sharmila S. Raut; Rupali S. Mantri; Saahil Parvez

doi:10.18203/2320-6012.ijrms20263493

Authors

Rajesh Dawre Department of Microbiology, Government Medical College, Akola, Maharashtra, India
Nazneen I. Malak Department of Microbiology, Government Medical College, Akola, Maharashtra, India
Sharmila S. Raut Department of Microbiology, Government Medical College, Bhandara, Maharashtra, India
Rupali S. Mantri Department of Microbiology, Government Medical College, Akola, Maharashtra, India
Saahil Parvez Department of Microbiology, Government Medical College, Akola, Maharashtra, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20263493

Keywords:

Candida albicans, Tropicalis, CHROM agar, Dalmau plate, Germ tube

Abstract

Background: Candida infections encompass a clinical spectrum ranging from superficial mucocutaneous lesions to life-threatening disseminated candidemia in immunocompromised hosts. Over the years, clinical distribution of Candida species has significantly evolved, shifting from the traditional dominance of Candida albicans to an increasing isolation rate of diverse non-albicans Candida (NAC) species. This study was conducted to isolate Candida spp. from various clinical specimens, to speciate and study the distribution of Candida albicans and non-albicans Candida spp. among various clinical specimens.

Method: A total of 464 samples were included in the study form January 2024 to June 2025. Samples included urine, blood, sputum, high vaginal swab, pus etc. Demographic details of patients were recorded. KOH, gram staining, germ tube techniques were done preliminarily. Culture on Sabourauds dextrose agar, morphology identification by Dalmau plate method and CHROM agar identification was done.

Results: Out of 464 Candida spp. isolated, highest infection (40.5%) seen in the 41-60 years age group, with female preponderance. The highest number of Candida isolates were obtained from urine samples 37.7% (n=175), followed by 28.2% (n=131) from sputum. non-albicans Candida spp. was 244 (53%) as compared to Candida albicans 220 (47%). C. troplicais predominated from all clinical samples and was commonest NAC spp. from urinary and respiratory tract.

Conclusion: The rise of non-albicans Candida species underscores a global shift toward higher drug resistance. Precise speciation is essential to identify these distinct resistance profiles, allowing clinicians to start appropriate empirical and target therapy.

References

Lopes JP, Lionakis MS. Pathogenesis and virulence of Candida albicans. Virulence. 2022;13(1):89-121.

Hankovszky P, Társy D, Öveges N, Molnár Z. Invasive Candida infections in the ICU: diagnosis and therapy. J Critical Care Med. 2015;1(4):129-39.

Talapko J, Juzbašić M, Matijević T, Pustijanac E, Bekić S, Kotris I, et. al Candida albicans—the virulence factors and clinical manifestations of infection. J Fungi. 2021;7:79.

Boekhout T, Aime MC, Begerow D, Gabaldón T, Heitman J, Kemler M, et al. The evolving species concepts used for yeasts: from phenotypes and genomes to speciation networks. Fungal Div. 2021;109(1):27-55.

Sarada D, Giddi S, Reddy M, Sisira D. Isolation and speciation of Candida from clinical samples in a tertiary care hospital at Kurnool, Andhra Pradesh, India. J Evol Medical Dental Sc. 2014;3(73):15431-7.

Standard Operating Procedures for Fungal Identification and Detection of Antifungal Resistance. 2nd Edition, 2019, Indian Council of Medical Research New Delhi, India. 2019.

Sharma M, Gupta G, Gupta GK, Kumari P, Gupta A. Identification of Candida species and its antifungal susceptibility from various clinical samples. Indian J Computer Sci Technol. 2025;6:61-5.

Amar CS, Ashish J, Hajare V, Belagali. Study of prevalence &antifungal susceptibility of Candida. Int J Pharm Bio-Sci. 2013;4(2):361-81.

Samyuktha AA, Saikumar C. Isolation, identification and speciation of Candida species from various clinical specimens in a tertiary care hospital in Chennai. Sch J Applied Med Sci. 2017;5:3460-8.

Anita, Kumar S, Pandya HB. Speciation and Antifungal Susceptibility Testing of Candida isolated from Immunocompromised Patients of a Tertiary Care Centre in Gujarat, India. J Pure Appl Microbiol. 2023;17(1):427-33.

Deorukhakar C, Saini S. Non albicans Candida species: a rewiew of epidemiology, pathogenicity and antifungal resistance. Pravara Med Rev. 2015;7(3):15.

Kaur R, Goyal R, Dhakad MS, Bhalla P, Kumar R. Epidemiology and virulence determinants including biofilm profile of Candida infections in an ICU in a Tertiary Hospital in India. J Mycol. 2014;4:64.

Naik SD, Pohekar JA, Kulkarni SS, Wyawahare AS, Mulay MV. Speciation of Candida isolated from various clinical samples & their susceptibility pattern at tertiary care hospital. Int J Pharm Res Technol. 2026;16(1):2585-92.

Roopa C, Biradar SK. Isolation of Candida and its speciation in various samples in a tertiary care hospital in North Karnataka, India. Int J Curr Microbiol App Sci. 2015;4:996-1000.

Sambrani PN, Chikkaraddi U, Divya A, Neema KS. Speciation and antifungal susceptibility testing of Candida isolated from various clinical specimens at a tertiary care centre: a cross-sectional study. National J Lab Med. 2025;14(3):7-12.