A comparative study of rapid urease test and dilute carbol fuchsin staining technique for diagnosis of Helicobacter pylori infection
Keywords:H. pylori, Rapid urease test, Dilute carbol fuchsin
Background: Helicobacter pylori (H.pylori) is a gram negative spiral bacilli associated with acid peptic disease (APD) and gastric carcinoma. A rapid urease test is the most common test performed for a quick clinical diagnosis with or without histopathological examination of antral biopsy specimens.
Methods: In this study we evaluated the utility of a simple dilute carbol fuchsin stain (DCF) for identifying the spiral or curved forms of H.pylori in the direct smears of antral biopsy samples for diagnosis of H.pylori infection and compared the results with rapid urease test (RUT).
Results: Sums of 100 cases were included in the study from which 61 (61%) were positive for urease production and shown typical spiral or curved bacilli by D.C.F stain.
Conclusions: DCF stain was found to be an excellent stain for direct microscopic evaluation and compared well with RUT.
Arora U, Aggarwal A, Singh K. Comparative evaluation of conventional methods and elisa based IgG antibodies detection for diagnosis of helicobacter pylori infection in cases of dyspepsis. Indian J Med Microbiol [Serial online]. 2003;21:46-8.
Prasad KN, Singhal S, Ayyagari A, Sharma S, Dhole TN. Association of Helicobacter pylori in patients of Non ulcer dyspepsia. Indian J Microbiol. 1991;31:415-8.
Barthal JS, Everett ED. Diagnosis of Campylobacter pylori infections: The gold standard and the alteration. Rev Inf Dis.1990;12(1):107-14.
Taylor DN, Blaser MJ. The epidemiology of Helicobacter pylori infection. Epidemiol Rev. 1991;13:42–59.
Kuipers EJ. Helicobacter pylori and the risk and management of associated diseases: gastritis, ulcer disease, atrophic gastritis and gastric cancer. Aliment Pharmacol Ther. 1997;11(1):71–88.
Mégraud F. Advantages and disadvantages of current diagnostic tests for the detection of Helicobacter pylori. Scand J Gastroenterol Suppl. 1996;31(215):57–62.
Parsonnet J, Shmuely H, Haggerty T. Fecal and oral shedding of Helicobacter pylori from healthy infected adults. JAMA. 1999;282:2240–5.
Walsh EJ, Moran AP. Influence of medium composition on the growth and antigen expression of Helicobacter pylori. J Appl Microbiol. 1997;83:67–75.
Dick JD. Helicobacter (Campylobacter) pylori: a new twist to an old disease. Annu Rev Microbiol. 1990;44:249–69. [PubMed /2252384>].
Rotimi O, Cairns A, Gray S, Moayyedi P, Dixon MF. Histological identification of Helicobacter pylori: comparison of staining methods. J Clin Pathol. 2000;53:756–9. [PMC free article /articles/PMC1731087/>] [PubMed /11064668>].
Laine L, Lewin DN, Naritoku W, Cohen H. Prospective comparison of H&E, Giemsa, and Genta stains for the diagnosis of Helicobacter pylori. Gastrointest Endosc. 1997;45:463–7. [PubMed /9199901>].