DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20151438

Role of Alcian blue (pH 2.5) histochemical stain in diagnostic thyroid pathology: how far is it helpful?

Seetu Palo, Dayananda S. Biligi

Abstract


Background: Controversies and pitfalls are well-known in diagnostic thyroid pathology. Since, the luminal surface of  neoplastic thyroid follicular cells is said to be covered by a glycoproteinaceous material, there is a need  to  investigate the efficacy of  mucin stains like Alcian blue (pH 2.5), which can be used as an adjunctive tool in difficult cases.

Methods: 60 thyroidectomy specimens, received at Department of Pathology, were included in this retrospective analytical study. Alcian blue histochemical (pH 2.5) staining was done on representative sections and assessed for positivity.

Results: Of the 60 cases analysed, all cases of papillary/nodular hyperplasia (n=10), follicular adenoma (n=14), follicular carcinoma (n=4) and medullary carcinoma (n=1) were negative for Alcian blue. Only 3/16 (18.7 %) cases of classical papillary carcinoma and 2/15 (13.3%) cases of follicular variant of papillary carcinoma showed positivity.

Conclusions: We got statistically insignificant results with Alcian blue (pH 2.5) staining, making it unreliable in diagnostic thyroid pathology.


Keywords


Alcian blue, Histochemical, Thyroid, Glycoprotein

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