In vivo antioxidant and hepatoprotective potential of Glycyrrhiza glabra extract on carbon tetra chloride (CCl4) induced oxidative-stress mediated hepatotoxicity

Varsha Sharma, R. C. Agrawal

Abstract


Objective: This study aimed to evaluate the antioxidant and hepatoprotective potential of Glycyrrhiza glabra hydromethanolic root extract against carbon tetra chloride (CCl4) induced oxidative-stress mediated hepatotoxicity in liver tissue of Swiss albino mice.

Background: Medicinal plants play a vital role for the development of new drugs. Glycyrrhiza glabra is a widely used medicinal plant. It has many phyto-constituents and active components, which can be used for many diseases.

Methods: For the antioxidant and hepatoprotective study, measurement of GSH, CAT, LPO bio-markers in Liver tissue of Swiss albino mice were taken. The animals were divided in six different groups each having 4 mice. The requisite dose of CCl4 was dissolved in appropriate solvent (1.5ml/kg body wt) and administrated as single i.p. dose per mice after 6 hr of last treatment of extract to the animals in each group. Mice were received orally administration of extract up to 7 days. Positive Control group received single i.p. injection of 1.5ml/kg body wt CCl4 in 0.9% saline.

Results: The results suggest that, the crude extract of root of G. glabra at the doses of 300 and 600mg/kg body wt. expressed significant hepatoprotective potential against CCl4 induced oxidative stress mediated hepatotoxicity in student ‘t’ test (p<0.05) at dose dependent manner in the Liver tissue of Swiss albino mice. G. glabra root extract alone has not induced hepatotoxicity.

Conclusion: Based on this study, It may be concluded that Glycyrrhiza glabra root extract possess hepatoprotective potential in Swiss albino mice.

 


Keywords


Antioxidant, CCl4,Catalase, Glutathione,Glycyrrhiza glabra, Hepatotoxicity, Intraperitoneal, Liver, Oxidative stress

Full Text:

PDF

References


Hill AF. Economic Botany: A textbook of useful plants and plant products (2ndedn.). McGraw Hill Book Company Inc, New-York, 1952.

Handa SS. Manual, TCDC International workshop cum training on herbal drugs. 1997.

Sandhir R, Gill KD. Hepatoprotective effects of Liv52 on ethanol induced liver damage in rats. Indian J Expt Biol. 1999;37:762-6.

Zore GB, Winston UB, Surwase BS, Meshram NS, Sangle VD, Kulkarni SS, Mohan Karuppayil S. Chemoprofile and bioactivities of Taverniera cuneifolia (Roth) Arn.: a wild relative and possible substitute of Glycyrrhiza glabra L. Phytomedicine. 2008; 15: 292–300.

Dong S, Inoue A, Zhu Y, Tanji M, Kiyama R. Activation of rapid signaling pathways and the subsequent transcriptional regulation for the proliferation of breast cancer MCF-7 cells by the treatment with an extract of Glycyrrhiza glabra root. Food Chem. Toxicol. 2007;45:2470-8.

Ammosov S, Litvinenko VI. Triterpenoids of Plants of Glycyrrhiza L. and Meristotropis Fisch. Et Mey Genuses. Pharm Chem J. 2003;37:83-94.

Jain S, Jain DK, Balekar N. In-Vivo Antioxidant activity of ethanolic extract of Mentha pulegium leaf against CCl4 induced toxicity in rats. Asian Pacific Journal of Tropical Biomedicine. 2012; 2(2):S737-S740.

Galighor AE and Kozloff EN. In: Essentials of practical Micro Technique. (2nd Eds.) Lea and Febiger, New York, 1976.

Ohkawa H, Ohishi N, and Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem. 1979;95:851.

Owens CWI and Belcher RV. Calorimetric micro method for the determination of glutathione. Biochem. 1965;94:705.

Aebi H. Catalase: In vitro. In: Colowick, SP, Kaplan, NO, (Eds.) Method in Enzymology, Academic press, NewYork, 1984;105:121-6.

Ward FM and Daly MJ. Hepatic Disease. In: Walker R and Edwards C (Eds.). Clinical Pharmacy and Therapeutics. Churchill Livingstone, New York, 1999; p. 195-212.

Ahsan R, Islam KM, Musaddik A and Haque E. Hepatoprotective Activity of Methanol Extract of Some Medicinal Plants Against Carbon Tetrachloride Induced Hepatotoxicity in Albino Rats. Global Journal of Pharmacology. 2009;3(3):116-22.

Naskar S, Islam A, Mazumder UK, Saha P, Haldar PK and Gupta M. In vitro and In vivo Antioxidant Potential of Hydromethanolic Extract of Phoenix dactylifera Fruits. J Sci Res. 2010;2(1):144-57.

Sharma V, Agrawal RC, Pandey S. Phytochemical screening and determination of anti-bacterial and anti-oxidant potential of Glycyrrhiza glabra root extracts. J Environ Res Develop. 2013;7(4A): 1552-8.

Yang H, Chen Y, Xu R, Shen W, Chen G. Clinical observation on the long-term therapeutic effects of traditional Chinese medicine for treatment of liver fibrosis. J Tradit China. 2000;20:247-50.