Laboratory diagnosis of urinary tract infections using diagnostics tests in adult patients

Akmal Hasan SK, Naveen Kumar T, Radha Kishan N, Neetha K

Abstract


Background:The primary aim of this study was to evaluate laboratory diagnosis of urinary tract infection using diagnostics tests in adult patients.

Methods:Among the diagnostic tests, urinalysis is useful mainly for excluding bacteriuria. For isolation of pathogenic bacteria semiquantitative culture techniques was used and biochemical tests were done to differentiate Gram +ve and Gram –ve bacteria.

Results: The incidence of pathogenic infection caused by Escherichia coli accounts for 216 cases (60%) followed by Pseudomonas, Staphylococcus aureus and Klebsiella.

Conclusion:Physicians should distinguish urinary tract infections caused by different organisms for an effective treatment and appropriate clinical information gives clues for better diagnostic evaluation and their susceptibility to antimicrobial agents as well addressing host factors that contribute to the occurrence of infection.


Keywords


Urinary tract infection, Urinalysis, Urine culture

Full Text:

PDF

References


Ananthanarayan R, Jayaram Paniker CK. Urinary Tract Infections. In: Kapil A, eds. Text Book of Microbiology. 9thed. Hyderabad, India: University Press; 2013: 671.

Josef Peter G, Ojan A, Michael B, Axel K. Incidence and clinical implication of nosocomial infections associated with implantable biomaterials-cathters, ventilator associated pneumonia urinart tract infections. GMS Krankenhhyg Interdiszip. 2011;6(1):Doc 18.

Weber G, Riesenberg K, Schlaeffer F, Peled N, Borer A, Yagupsky P. Changing trends in frequency and antimicrobial resistance of urinary pathogens in outpatient clinics and a hospital in southern Israel. Eur J Clin Microbiol Infect Dis. 1997;16:834-8.

Stamm WE, Counts GW, Running KR. Diagnosis of coliform infection in acutely dysuric women. N Eng J Med. 1982;307:463-8.

Bronsema DA, Adams JR, Pallares R, Wenzel RP. Secular trends in rates and etiology of nosocomial urinary tract infections at a university hospital. J Urol. 1993;150:414-6.

Stamm WE, Counts GW, Running KR. Diagnosis of coliform infection in acutely dysuric women. N Eng J Med. 1982;307:463-8.

Clarridge JE, Johnson JR, Pezzlo MT. Cumitech 2B: Laboratory diagnosis of urinary tract infections. Coordinating ed. Washington, DC: American Society for Microbiology; 1998.

Morris RW, Watts MR, Reeves DS. Perineal cleansing before midstream urine, a necessary ritual? Lancet. 1979;2:158-9.

Jefferson H, Dalton HP, Escobar MR, Allison M. Transportation delay and the microbiological quality of clinical specimens. Am J Clin Pathol. 1975;64:689-93.

Hindman R, Tronic B, Bartlett R. Effect of delay on culture of urine. J Clin Microbiol. 1976;4:102-3.

Wheldon DB, Slack M. Multiplication of contaminant bacteria in urine and interpretation of delayed culture. J Clin Pathol. 1977;30:615-9.

Bale MJ, Matsen JM. Evidence against the practicality and cost-effectiveness of a gram-positive coccal selective plate for routine urine cultures. J Clin Microbiol. 1981;14:617-9.

Carroll KC, Hale DC, Von Boerum DH, Reich GC, Hamilton LT, Matsen JM. Laboratory evaluation of urinary tract infections in an ambulatory clinic. Am J Clin Pathol. 1994;101:100-3.

Murray P, Traynor P, Hopson D. Evaluation of microbiological processing of urine specimens: comparison of overnight versus two-day incubation. J Clin Microbiol. 1992;30:1600-1.

Aspevall O, Osterman B, Dittmer R, Sten L, Lindback E, Forsum U. Performance of four chromogenic urine culture media after one or two days of incubation compared with reference media. J Clin Microbiol. 2002;40:1500-3.

William H, Benjamin Jr, Ken Waites B, Stephen Moser A. The MB/Bac T is asensitive Method of Isolating Mycobacterium tuberculosis from Clinical Specimens in a Laboratory with a Low Rate of Isolation. J Clin Microbiology. 2000;38(8):3133-34.

Carroll KC, Hale DC, Von Boerum DH, Reich GC, Hamilton LT, Matsen JM. Laboratory evaluation of urinary tract infections in an ambulatory clinic. Am J Clin Pathol. 1994;101:100-3.