Efficacy of antibacterial activity of garlic cloves from Tamil Nadu and Jowai region

M. Anitha, J. Hemapriya

Abstract


Background:The development of antibiotic resistance has become a global health challenge which is causing ineffectiveness of the available antibacterial agents leading to increase in diseases and death rate. Therefore this study intends to investigate the antibacterial action of Aqueous Garlic Extract (AGE) against 9 multidrug-resistant gram-positive and gram-negative bacterial isolates, including Staphylococcus aureus, Enterococcus species, ß hemolytic streptococcus, Pseudomonas aeruginosa, Proteus mirabilis, Klebsiella pneumoniae, Escherichia coli, Vibrio cholerae and Serretia marscenes.

Methods:Antibacterial activity of different concentrations of AGE by well-diffusion method was recorded by measuring the diameter of zone of inhibition. The Tamil Nadu garlic cloves as well as Jowai region garlic cloves showed antibacterial activity against both GPC and GNR.

Results: The maximum zone of inhibition was observed in Tamil Nadu garlic than that of Jowai region, but the only bacteria which showed a better zone of inhibition with Jowai region than Tamil Nadu garlic was Pseudomonas aeruginosa.

Conclusion:Thus our study reveals that garlic not only makes the food more spicy & edible with its flavour but can also be used as an effective antibacterial agents against MDR gram positive & gram negative bacteria.

 


Keywords


Bacterial isolates, Antibacterial activity, Tamil Nadu garlic and Jowai region garlic extracts

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References


Al-Bari MAA, Khan A, Islam MR, Kudrat-E-Zahan E, Rahman MMS, Ul-Islam MA et al. Isolation and in vitro antimicrobial activities of ethyl acetate extract from Streptomyces bangladeshiensis. Res J Microbiol. 2007;2:272-7.

Menghani E, Pareek A, Negi RS, Ojha CK. Search for antimicrobial potential from certain Indian medicinal plants. Res J Med Plants. 2011;5:295-301.

Premanath R, Sudisha J, Lakshmi Devi N, Aradhya SM. Antibacterial and antioxidant activities of Fenugreek (Trigonella foenum graecum L.) leaves. Res J Med Plants. 2011;5:695-705.

Tijjani MB, Bello IA, Aliyu AB, Olurishe T, Maidawa SM, Habila JD et al. Phytochemical and antibacterial studies of root extract of Cochlospermum tinctorium A. rich. (Cochlospermacease) Res J Med Plants. 2009;3:16-22.

Darbyshire B., Henry R. J., Differences in fructan content and synthesis in some Allium species. New Phytol. 1981;87:249-56.

Koch H. P., Lawson L. D. Garlic, the science and therapeutic application of Allium sativum L. and related species. In: Retford D. C., eds. Lippincott Williams & Wilkins. Baltimore: Wolters Kluwer; 1996: 1-233.

Alli JA, Boboye BE, Okonko IO, Kolade AF, Nwanze JC. In-Vitro assessments of the effects of garlic (Allium sativum) extract on clinical isolates of Pseudomonas aeruginosa and Staphylococcus aureus. Adv Appl Sci Res. 2011;2(4):25-36.

Ram Kumar Pundir, Pranay Jain, Chetan Sharma. Antimicrobial activity of ethanolic extracts of Syzygium aromaticum and Allium sativum. Ethnobotanical Leaflets. 2010;14:344-60.

Bandna Chand. Antibacterial effect of garlic (Allium sativum). J of Microbiol, Biotech and Food Sci. 2013:2(4):2481-91.

Shoeb H. Antimicrobial susceptibility testing (Kirby Bauer) animation. Microbe Library, 2008. Available at: http://www.microlibrary.org/asmonly/details.asp?id=2754&lang. Accessed 25 August 2009.

Clinical and laboratory standards institute. Performans standards for antimicrobial susceptibility testing. Seventeenth Informational Supplement. 2007;CLSI 26:M100-S117.

Srinivasan Durairaj, Sangeetha Srinivasan. In vitro antibacterial activity and stability of garlic extract at different pH and temperature. Electro J of Biol. 2009;5(1):5-10.