Serum vitamin D and vitamin D receptor gene polymorphism in Moroccan patients with systemic lupus erythematosus
DOI:
https://doi.org/10.18203/2320-6012.ijrms20170467Keywords:
Gene polymorphism, Lupus, Vitamin D, Vitamin D receptorAbstract
Background: Vitamin D plays an important role in the immunomodulation and could be involved in the development of autoimmune diseases such as systemic lupus erythematous (SLE). The study of the polymorphism of the Vitamin D Receptor (VDR) gene may be of interest in explaining the pathophysiology of SLE.
Methods: In this study, we aimed to examine the characteristics of VDR gene BsmI polymorphism for the first time in Moroccan patients with SLE and their relationship with clinical manifestations of the disease. We also measured the serum level of 25-hyroxyvitamin D3 to assess its relation to such polymorphism.
Results: The study included 66 SLE patients and 91 healthy controls. Our results showed that there were no differences observed in VDR genotypes and allelic distribution within the two groups. Both groups were in Hardy-Weinberg equilibrium, with no significant P values for the observed and expected genotype frequencies. 25-hyroxyvitamin D3 serum levels were the same in the two groups.
Conclusions: Based on the results of the present study. We cannot verify any association between VDR gene BsmI polymorphism and SLE. This polymorphism could not be regarded as a genetic marker of the SLE. A larger study examining BsmI and other VDR gene polymorphisms is needed.
References
Pons-Estel GJ, Alarcón GS, Scofield L, Reinlib L, Cooper GS. Understanding the epidemiology and progression of systemic lupus erythematosus. Semin Arthritis Rheum. 2010;39:57-68.
Lee HS, Bae SC. What can we learn from genetic studies of systemic lupus erythematosus? Implications of genetic heterogeneity among populations in SLE. Lupus. 2010;19:1452-9.
Moser KL, Kelly JA, Lessard CJ, Harley JB. Recent insights into the genetic basis of systemic lupus erythematosus. Gene Immun. 2009;10:373-9.
Cantorna MT, Snyder L, Lin YD, Yang L. Vitamin D and 1,25(OH)2D regulation of T cells. Nutrients. 2015;7:3011-21.
Jeffery LE, Burke F, Mura M, Zheng Y, Qureshi OS, Hewison M et al. 1,25-dihydroxyvitamin D3 and interleukin-2 combine to inhibit T cell production of inflammatory cytokines and promote development of regulatory T cells expressing CTLA-4 and FoxP3. J Immunol. 2009;183:5458-67.
Ritterhouse LL, Crowe SR, Niewold TB, Kamen DL, Macwana SR, Roberts VC et al. Vitamin D deficiency is associated with an increased autoimmune response in healthy individuals and in patients with systemic lupus erythematosus. Ann Rheum Dis. 2011;70:1569-74.
Cutillas-Marco E, Morales-Suárez-Varela MM, Marquina-Vila A, Grant WB. Serum 25-hydroxyvitamin D levels in patients with cutaneous lupus erythematosus in a Mediterranean region. Lupus. 2010;19:810-4.
Mandal M, Tripathy R, Panda AK, Pattanaik SS, Dakua S, Pradhan AK et al. Vitamin D levels in Indian systemic lupus erythematosus patients: association with disease activity index and interferon alpha. Arthritis Res Ther. 2014;16:R49.
Mostowska A, Lianeri M, Wudarski M, Olesińska M, Jagodziński PP. Vitamin D receptor gene BsmI, FokI, ApaI and TaqI polymorphisms and the risk of systemic lupus erythematosus. Mol Biol Rep. 2013;40:803-10.
Erten G, Kalkan M, Gazioğlu SB, Akdeniz N, Ozkok E, Vural B. TaqI, FokI, and ApaI Polymorphisms in the Vitamin D Receptor in Behçet's disease in Turkish population. Dis Markers. 2016;2016:7475080.
Tan EM, Cohen AS, Fries JF. The 1982 revised criteria for the classification of systemic lupus erythrematosus. Arthritis Rheum. 1982;25:1271-7.
Ceccarelli F, Perricone C, Borgiani P, Ciccacci C, Rufini S, Cipriano E, et al. Genetic factors in systemic lupus erythematosus: contribution to disease phenotype. J Immunol Res. 2015;2015:745647.
Vasile M, Corinaldesi C, Antinozzi C, Crescioli C. Vitamin D in autoimmune rheumatic diseases: A view inside gender differences. Pharmacol Res. 2016;31:228-41.
Vollmert C, Illig T, Altmüller J, Klugbauer S, Loesgen S, Dumitrescu L et al. Single nucleotide polymorphism screening and association analysis-exclusion of integrin beta 7 and vitamin D receptor (chromosome 12q) as candidate genes for asthma. Clin Exp Allergy. 2004;34:1841-50.
Ozaki Y, Nomura S, Nagahama M, Yoshimura C, Kagawa H, Fukuhara S. Vitamin-D receptor genotype and renal disorder in Japanese patients with systemic lupus erythematosus. Nephron. 2000;85(1):86-91.
Huang CM, Wu MC, Wu JY, Tsai FJ. Non-association of vitamin D receptor gene start codon FokI polymorphism in Chinese patient with systemic lupus erythematous. J Rheumatol. 2002;29:1211-3.
Ozaki Y, Nomura S, Nagahama M, Yoshimura C, Kagawa H, Fukuhara S. Vitamin-D receptor genotype and renal disorder in Japanese patients with systemic lupus erythematosus. Nephron. 2000;85:86-91.
Azab SF, Ali YF, Farghaly MA, Hamed ME, Allah MA, Emam AA et al. Vitamin D receptor gene BsmI polymorphisms in Egyptian children and adolescents with systemic lupus erythematosus. Medicine (Baltimore). 2016;95:e5233.
Huang CM, Wu MC, Wu JY, Tsai FJ: Association of vitamin D receptor gene BsmI polymorphisms in Chinese patients with systemic lupus erythematosus. Lupus. 2002;11:31-4.
Monticielo OA, Brenol JC, Chies JA, Longo MG, Rucatti GG, Scalco R et al. The role of BsmI and FokI vitamin D receptor gene polymorphisms and serum 25-hydroxyvitamin D in Brazilian patients with systemic lupus erythematosus. Lupus. 2012;21:43-52.
Sakulpipatsin W, Verasertniyom O, Nantiruj K, Totemchokchyakarn K, Lertsrisatit P, Janwityanujit S. Vitamin D receptor gene BsmI polymorphisms in Thai patients with systemic lupus erythematosus. Arthritis Res Ther. 2006;8:R48.
Holick MF. Optimal vitamin D status for the prevention and treatment of osteoporosis. Drugs Aging. 2007;24:1017-29.
Mok CC. Vitamin D and systemic lupus erythematosus: an update. Expert Rev Clin Immunol. 2013;9:453e63.
Borba VZ, Vieira JG, Kasamatsu T, Radominski SC, Sato EI, Lazaretti-Castro M. Vitamin D deficiency in patients with active systemic lupus erythematosus. Osteoporos Int. 2009;20:427e33.
Handor N, Elalami S, Bouabdellah M, Srifi A, Esselmani H, Benchekroun L et al. Dosage of 25-OH vitamin D: experience of central clinical biochemistry laboratory of Ibn Sina Hospital. Pan Afr Med J. 2014;17:152.
Bouaddi I, Rostom S, El Badri D, Hassani A, Chkirate B, Abouqal R, et al. Vitamin D concentrations and disease activity in Moroccan children with juvenile idiopathic arthritis. BMC Musculoskelet Disord. 2014;15:115.
El Maataoui A, Biaz A, El Machtani S, Bouhsain S, Dami A, El Maghraoui A et al. Vitamin D status in healthy Moroccan men and women aged 50 years and older: a cross-sectional study. Arch Osteoporos. 2016;11:24.
