An innovative approach for manual preparation of slides of compact bone tissue
Keywords:
Bone, Microtome, Haversian systemAbstract
Background: Bone is a rigid form of connective tissue in which the extracellular matrix is impregnated with inorganic salts, mainly calcium phosphate and carbonate, that provide hardness. Because of its hardness, bone is difficult to cut in the microtome; therefore, special techniques must be used for its study.
Methods: Grinding of transverse sections of compact bone was done by using flint paper (size 0) which is simple, extremely cheap, rapid and easily available.
Results: Under low power magnification, we observed a complete haversian system with volkmann’s canal and interstitial lamellae.
Conclusions: This method of preparation of ground bone slides is not only helpful to undergraduate and post graduate students but also to osteoarchaeologists, palaeopathologists and forensic anthropologists.
References
Junqueira IC, Carneiro J, Contopoulos A eds. Bone. In: Basic histology. 2nd ed. Los Altos, California: Lange Publishers; 1977:119-39.
Singh I. Bone. In: Vasudeva N, Mishra S eds. Textbook of Human Histology with colour atlas and practical guide. 7th ed. New Delhi: Jaypee; 2014:87-102.
Maat GJR, Van Den Bos RPM, Aarents M. Manual preparation of ground sections for the microscopy of natural bone tissue: update and modification of Frost's ‘rapid manual method’. Int J Osteoarchaeol 2001;11:366–74.
Callis GM. Bone. In: Bancroft JD, Gamble M eds. Theory and practice of Histological technique. 6th ed. Philadelphia: Churchill Livingstone Elsevier; 2008: 333-64.
Microscopy of bone and step-by-step sample preparation, 2012. Available at: www.microscopy-uk.org.uk/mag/artmay12/ug-Bone.html. Accessed 10 August 2014.
Mukherjee P, Roy H, Mallik A. A quick and easy approach to prepare ground bone slides from undecalcified skeleton. J Anat Soc Ind 2012;61(2): 277-79.