Study of the red cell indices, hemogram and platelet variations in anaemic (<10gm%) patients by automatic cell counter in a tertiary care centre, Ahmednagar, Maharashtra, India

Saili U. Jadhav, Sadhana Khaparde


Background: Anemia is not a disease but it is the expression of underlying diseases and from the treatment point of view, it is necessary to identify the cause of anemia. The definition of anemia is as decrease in the number of red blood cells or the decreased percentage of hemoglobin in the blood. Anemia is such an important issue affecting a large population in India as well as worldwide, Hence, the present study was conducted to evaluate the red cell indices, haemogram and study the relation of platelet count with anaemia in anaemic (<10gm%) patients by automatic cell counter in Vikhe Patil Medical hospital.

Methods: It was a prospective cross-sectional study. The sample size of research project was 100 patients, included according to the inclusion and exclusion criteria.

Results: In present study, equal numbers of patients suffer from macrocytic normochromic anemia and hyperchromic anemia, that is, 8 patients of each. Out of 100 patients, 61% patients showed decreased platelet count. Maximum percentage of anaemic patients showed decreased platelet count in the range of 2.5-1.5 lakhs. Least percentage of patients (16%) showed platelet count less than 0.5 lakhs/cmm3.

Conclusions: Screening for anaemia, treatment of anaemic women, and availability and use of food fortification (wheat flour with iron and folic acid), milk, sugar and salt with iron to build long term iron stores remains the key to reduce anemia in adolescent and pregnant women. Consumption of cheap iron-rich foodstuffs should be promoted. Effective poverty alleviation and hookworm prevention programs are also important.


Anemia, Haemogram, Platelets

Full Text:



Vos T, Flaxman AD, Naghavi M, Lozano R, Michaud C, Ezzati M, et al. Years lived with disability (YLDs) for 1160 sequelae of 289 diseases and injuries 1990-2010: a systematic analysis for the Global Burden of Disease Study 2010. Lancet. 2012;380(9859):2163-96.

Nayak R, Rai S. Rapid Review of Hematology. 1st ed. New Delhi: Jaype Brothers Medical Pub. 2014:11-2.

Glassman AB, Anemia: diagnosis and clinical conditions. In: Harmering DE, Ed. Clinical hematology and fundamentals of hemostasis, 3rd ed. Philadelphia FA; 1997:71-79.

Lee GR. Anemia: general aspects. In: Lee GR, Former L, Lukens J, eds. Wintrobe’s clinical hematology 10th Ed, Philadelphia: Lippincott Williams and Wilkins; 1999:901-907.

Adams-graves P. Approach to anemia. In: Ling F, Duff F, eds. Obstrectics and gynecology: principles for practice. New York: McGraw-Hill; 2001: L751-764.

Lee GR. Anemia: A diagnostic strategy. In: Lee GR, Former J, Lukens J, eds. Wintrobe’s clinical hematology, 10th ed. Philadelphia: Lippincott Williams and Wilkins; 1999:980-940.

Erslev AJ. Clinical manifestations and classifications of erythrocytes disorders. In: Beutler E, Lichtman MA, Coller, eds. Williams Hematology, 6th ed, New York: McGam Hill; 2001:369-374.

Janz TG, Johnson RL, Rubenstein SD. (Nov 2013). Anemia in the emergency department: evaluation and treatment. Emerg Med Pract. 2013;15(11):1-15

World Health Organization. The World Health Report 2002: Reducing risks, promoting healthy life. Geneva, World Health Organization. 2002.

Verma P, Singh S, Ghildiyal A, Kumar A, Krishna A. Prevalence of anemia in adults with respect to socio-demographic status blood groups and religion in north Indian population. Int J Biol Med Res. 2012;3(4):2422-8.

Bansal B, Takkar J, Soni ND, Agrawal DK, Agrawal S. Comparative study of prevalence of anemia in Muslim and non-Muslim pregnant women of western Rajasthan. Int J Res Health Sci. 2013;1(2):47-52

Tiwari M, Kotwal CJ, Kotwal A, Mishra MP, Dutta V, Chopra S. Correlation of hemoglobin and red cell indices with serum ferritin in Indian women in second and third trimester of pregnancy. Med J Armed Forces India. 2013;69(1):31-6.

Joseph IJ, Jeffrey KT. Anemia in children. Am Fam Phys. 2001;64(8):1379.

Lee GR, Herbert V. In: Lukens J, Paraskevas P, Greer JP, Rodgers GM, eds. Wintrobe’s clinical hematology. Baltimore, Maryland USA: William and Wilkins. 1998:228-266.

de Benoist B, McLean E, Egli I, Cogswell M. WHO. WHO global database on anaemia. 2008. Available from: http:// whqlibdoc. publications/ 2008/9789241596657_eng.pdf (Retrieved on July February 6th, 2014).

Ezzati M, Lopus AD, Dogers A, Vander HS, Murray C. Selected major risk factors and global and regional burden of disease. Lancet. 2002;360:1347-60.

Beard JL, Hendricks, MK, Perez EM, Murray-Kolb LE, Berg A, Vernon-Feagans L, et al. Maternal iron deficiency anemia affects postpartum emotions and cognition. J Nutr. 2005;135(2):267-72.

Rammohan A, Awofeso N, Robitaille MC. Addressing female iron-deficiency anaemia in india: is vegetarianism the major obstacle? ISRN Public Health, 2012;2012:1-8.

Kadikoylu G, Yavasoglu I, Bolaman Z, Senturk T. Platelet parameters in women with iron deficiency anemia. J Natl Med Assoc. 2006;98(3):398-402.