DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20171238

Molecular characterization of corneal ulcers causing Staphylococcus aureus

Deepika N. Jain, Vilas A. Kamble

Abstract


Background: The human eye is one of the most remarkable sensory systems. Human beings gather most of the information about the external environment through their eyes and thus rely on sight more than on any other sense, with the eye being the most sensitive organ we have. Molecular characterization of Staphylococcus aureus from the cases of corneal ulcers.

Methods: A total of 300 samples of corneal ulcers collected from various ophthalmology hospitals, government hospital and clinical laboratories of different places of Maharashtra, India. The Isolates were identified based on the colony morphology and biochemical reaction. The isolates were subjected for antibiotic sensitivity test and perform its molecular characterization.

Results: In present study, 39 coagulase positive Staphylococcus aureus, pathogenic bacteria isolated from corneal ulcers.

Conclusions: Staphylococcus aureus is one of the most significant pathogens in bacterial keratitis. Early diagnosis and prompt treatment are needed to minimize the possibility of permanent vision loss and reduce structural damage to the cornea.


Keywords


Antibiotic sensitivity test, Corneal ulcer, Molecular characterization, Staphylococcus aureus

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References


Chatterjee BM. Roy IS, ed. Hand book of Ophthalmology, Fourth edition. CBS publication. 1988;85-113.

National programme for control of blindness (NPCB), New Delhi. Government of India. Ministry of Health and family welfare. 12th five-year plan. 2012-2017:1-6,

Govind NL, Damor JR, Padhiyar NG, Javadekar TB. To study the causative organism responsible for corneal ulcer in S.S.G Hospital Vadodara, Gujarat, Nat J Comm Med. 2011;2(2):237-40.

Song HY, Qiu BF, Liu C, Zhu SX, Wang SC, Miao J, et al. Identification of causative pathogens in mouse eyes with bacterial keratitis by sequence analysis of 16S rDNA libraries, Exp Anim. 2015;64(1):49-56.

Frohlich SJ, de Kaspar MH, Grasbon T, Mohring C, Klauss V, Kampik A. Bacterial keratitis. Microbiological analysis as a principle for therapeutic recommendations, Ophthalmology. 1999;96:459-64.

Bourcier T, Thomas F, Borderie V, Chaumeil C, Laroche L. Bacterial keratitis: predisposing factors, clinical and microbiological review of 300 cases. Br J Ophthalmol. 2003;87:834-8.

Al-Mujaini A, Al-Kharusi N, Thakral A, Wali UK. Bacterial keratitis: perspective on epidemiology, clinico-pathogenesis, diagnosis and treatment. Sultan Qaboos Univ Med J. 2009;9(2):184-95.

Harris LG, Foster SJ, Richards RG. An introduction to Staphylococcus aureus, and techniques for identifying and quantifying S. aureus adhesions in relation to adhesion to biomaterials. Europian Cells Mater. 2002;4:39-60.

Ranginee C, Sasmita P, Sharma S, Singh DV. Pana M, ed. Staphylococcal infection, antibiotic resistance and therapeutics, antibiotic resistant bacteria- a continuous challenge in the new millennium. Intech publishers; 2012):576.

Marquart ME, O’Callaghan RJ. Infectious keratitis: secreted bacterial proteins that mediate corneal damage. J Ophthalmol. 2013;2013:9.

Jett BD, Gilmore MS. Internalization of Staphylococcus aureus by human corneal epithelial cells: role of bacterial fibronectin-binding protein and host cell factors. Infect Immun. 2002;70(8):4697-700.

Truant AL. Manual of commercial methods in clinical microbiology. American Society for Microbiology Washington, D.C., 2002:270.

Kaye SB, Rao PG, Smith G, Scott JA, Hoyles S, Morton CE, Willoughby C, Batterbury1 M, Harvey G. Simplifying Collection of corneal specimens in cases of suspected bacterial keratitis. J Clin Microbiol. 2003;41(7):3192-7.

Bauer AW, Kirby WMM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45:493-6.

Central Laboratory Standard Institute (CLSI). Performance standards for antimicrobial disc susceptibility tests, Approved standards 10th ed. CLSI document M02-A10, 2015;29(1).

Edgar RC. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res. 2004;32(5):1792-7.

McLeod SD, LaBree LD, Tayyanipour R. The importance of initial management in the treatment of severe infectious corneal ulcers. Ophthalmology. 1995;102:1943-8.

Vajpayee RB, Dada T, Saxena R. Study of the first contact management profile of cases of infectious keratitis: a hospital-based study. Cornea. 2000;19:52-6.

Musch DC, Sugar A, Meyer RF. Demographic and predisposing factors in corneal ulceration, Arch Ophthalmol. 1983;101:1545-8.

Parmar P, Salman A, Kalavathy CM, Kaliamurthy J, Thomas PA, Jesudasan CA. Microbial keratitis at extremes of age. Cornea. 2006;25(2):153-8.

Kadir AJ, Samsudin A, Fauzi A, Abidin ZZ. Review of corneal ulcers at University Malaya Medical Centre. Int J Ophthalmol. 2008;8:2376-80.

Rhem MN, Lech EM, Patti JM, Mcdevitt D, Ho MOK, Jones DB, Wilhelmus KR. The collagen-binding adhesion is a virulence factor in Staphylococcus aureus Keratitis. Infect Immun. 2000;68(6):3776-9.

Garg PG, Rao GN. Corneal ulcer: diagnosis and management. Comm Eye Health. 1999;12(30):21-3.

Kaatz GW, Seo SM. Mechanism of ciprofloxacin resistance in Pseudomonas aeruginosa, J Infect Dis. 1988;58:537.

Rachida B, Jeffrey D, Theresa R, James B. Proteomics in the study of bacterial keratitis. Proteomes. 2015;3:496-511.