Effect of wearing complete dental prosthesis on candidal count

Ankur Bhargava, Sonal Saigal


Background: Infection due to fungi have increased dramatically in recent years and are of prime importance because of the rising number of immunocompromised patients, such as cancer patients receiving chemotherapy, and human immunodeficiency virus infected patients. The aim of the analysis was to compare the candidal count at the time of insertion and after one month of wearing complete dentures.

Methods: It was a quasi-experimental study which consisted of forty edentulous patients with no previous denture history. They obtained new complete dentures. Samples were taken using the oral rinse technique and cultured on sabouraud agar for fungal growth and cultured positive samples had undergone for germ tube test in serum media.

Results: Candidal colonies were detected and counted as colony forming units at the time of insertion and after one month of wearing complete dentures. Change in candidal count was significantly higher after one month of wearing complete dentures (p=0.0001). Difference in candidal count was significantly higher in females than males.

Conclusions: Candidal count was significant after wearing complete dentures and this should be considered as an important factor that can pre-dispose the patients to candida induced denture stomatitis.


Candidal count, Denture stomatitis, Sabouraud agar

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Coenye T, Prijck KD, Nailis H, Nelis HJ. Prevention of Candida albicans biofilm formation. Open Mycol J. 2011;5:9-20.

Felton D, Cooper L, Duqum I, Minsley G, Guckes A, Haug S, et al. Evidence-based guidelines for the care and maintenance of complete dentures: A publication of the American College of Prosthodontists. J Am Dent Assoc. 2011;142:S1-20.

Brondani MA, Samim F, Feng H. A conventional microwave oven for denture cleaning: a critical review. Gerodontology. 2012;29:6-15.

Dangi YS, Soni ML, Namdeo KP. Oral candidiasis: a review. Int J Pharm Pharm Sci. 2010;2:3641.

Abraham CM. Advances and emerging techniques in the identification, diagnosis and treatment of oral candidiasis. Open Path J. 2011;5: 8-12.

Farah CS, Lynch N, McCullough MJ. Oral fungal infections: an update for the general practitioner. Aust Dent J. 2010;55:48-54.

Gleiznys A, Zdanavičienė E, Žilinskas J. Candida albicans importance to denture wearers. A literature review. Stomatol Baltic Dent Maxillofacial J. 2015;17:54-66.

Salim N, Moore C, Silikas N, Satterthwaite J, Rautemaa R. Candidacidal effect of fluconazole and chlorhexidine released from acrylic polymer. J Antimicrob Chemother. 2013;68:587-92.

Marra J, Paleari AG, Rodriguez LS, Leite ARP, Pero AC, Compagnoni MA. Effect of an acrylic resin combined with an antimicrobial polymer on biofilm formation. J Appl Oral Sci. 2012;20:643-8.

Lazarin AA, Zamperini CA, Vergani CE, Wady AF, Giampaolo ET, Machado AL. Candida albicans adherence to an acrylic resin modified by experimental photopolymerised coatings: an in vitro study. Gerodontology. 2014;31:25-33.

Sharmila R, Muralidharan NP. Angular cheilitis in complete dentures. J Pharm Sci Res. 2015;7(8):598-9.

Pires FR, Santos EB, Bonan PR, De Almeida OP, Lopes MA. Denture stomatitis and salivary Candida in Brazilian edentulous patients. J Oral Rehab. 2002;29:1115-9.

Figueiral MH, Azul A, Pinto E, Fonseca PA, Branco FM, Scully C. Denture-related stomatitis: identification of aetiological and predisposing factors- a large cohort. J Oral Rehab. 2007;34:448-55.

Alkumru HN, Beydemir K. The prevalence of Candida albicans in complete denture and removable partial denture wearers: a comparative study. J Marmara Univ Dent Fac. 1992;1:218-22

Davenport JC. The oral distribution of candida in denture stomatitis. Br dent J. 1970;129:151-6.

Kulak Y, Arikan A. Aetiology of denture stomatitis. J Marmara Univ Dent Fac. 1993;1:307-14.

Campisi G, Pizzo G, Milici ME, Mancuso S, Margiotta V. Candidal carriage in the oral cavity of human immunodeficiency virus-infected subjects. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2002;93:281-6.

Ikebe K, Morii K, Matsuda K, Hata K, Nokubi T. Association of candidal activity with denture use and salivary flow in symptom- free adults over 60 years. J Oral Rehab. 2006;33:36-42.

Coelho CM, Sousa YT, Dare AM. Denture-related oral mucosal lesions in a Brazilian school of dentistry. J Oral Rehab. 2004;31:135-9.

Abu-Elteen KH, Abu-Alteen RM. The prevalence of Candida albicans populations in the mouths of complete denture wearers. New Microbiol. 1998;21:41-8.