A comparative study of lip print patterns in monozygotic and dizygotic twins

Bhawna Thakur, Buddhadeb Ghosh, Nidhi Puri, Rimple Bansal, Suman Yadav, Ravi Kant Sharma


Background: External surface of lips has many elevations and depressions forming a characteristic pattern called lip prints, the study of which is known as cheiloscopy. This is unique for the individuals like in fingerprints.

Methods: The present study was conducted in 40 pairs of twins and their families to evaluate the possibility of variation of lip print patterns in twins and their parents to find out any similarity among twins and their families.  The twins were taken as the primary subject and their parents as the secondary subjects.

Results: The subjects with congenital lip deformity and any inflammation were excluded from the study. The lip prints were taken on the cello tape and highlighted with the black printer powder of Oddyessy make. the photographs were taken for the permanent record. The prints were studied on Adobe Photoshop 7.0 software and classified under Tsuchihashi classification.

Conclusions: Comparison of lip prints showed that they are unique to each individual and among twins revealed that they are similar but not identical and their characteristics resembled either parent. Since the lip print patterns are unique, lip print analysis can be considered as a tool for personal identification.


Cheiloscopy, Inheritance, Lip prints, Twins

Full Text:



Tsuchihashi Y. Studies on personal identification by means of lip prints. Forensic Sci.1974;3:233-48.

Vats Y, Dhall AK, Kapoor AK. Heritability of lip print patterns among north Indian population. J Forensic Res. 2011;27-9.

Caldas IM, MagahaesT, Afonso A. Establishing identity using cheiloscopy and palatoscopy. Forensic Sci Int. 2007;165:1-9.

Aggarwal A. Forensic Files. Available from: accessed on 15th january,2012.

Vahanwala SP, Parekh BK, Pagare SS. Study of lip prints for sex identification. Medico-Legal Update.2005;5(3):7-9.

Reddy LVK. Lip prints: An overview in forensic dentistry. Journal of Advanced Dental Research. 2011;I (I):17-20.

Randhawa K, Narang RS, Arora PC. Study of the effect of age changes on lip print patterns and its reliability in sex determination. J Forensic Odontostomatol. 2011; 29(2):45-51.

Kumar P, Mastan KMK, Patil S. Cheiloscopy: Efficacy Of fluorescent dye over lysochrome dye in developing invisible lip prints. IJCD. 2010;3:3-6.

Chorus M. The lip as a biometric. Pattern Analysis and Applications. 2010;13(1):105-12.

Suzuki K, Tsuchihashi Y. Personal identification by means of lip prints. J of Forensic Med. 1970;17:52-7.

Hirthe L, Gottsche H, Goedde HW. Lip prints- variability and genetics. Humangenetik. 1975; 30(1):47-62.

Schnuth, Lee M. Lip prints. The FBI Law Enforcement Bulletin.1992.

Jaishankar S, Jaishankar N, Shanmugham S. Lip prints in personal identification. JIADS. 2010; 1(4):23-6.

Patel S, Paul S, Madhusudan AS, Ramesh G, Sowmya GV. A study of lip prints in relation to gender, family and blood group. International Journal of Oral & Maxillofacial Pathology. 2010; 1(1):4-7.

Venkatesh R, David MP. Cheiloscopy: An aid for personal identification. Journal of Forensic Dental Sciences. 2011;3(2):67-70.

Maheswari TNU, GnanasundaramN. Role of lip prints in Personal Identification and Criminalization. Anil Aggarwal’s Internet Journal of Forensic Medicine and Toxicology 2011;12(1):21serial online 2011;(1).

Ragab AR, Dakroori SA, Rahman RHA. Characteristics patterns of lip prints in Egyptian population sample at Dakhalia Governorate. International Journal of legal medicine. 2012; 127(2):521-7.

Saraswati TR, Mishra G, Rangnathan K. Study of lip prints. J of Forensic Dental Sciences. 2009; 1(1):28-31.

Augustine J, Barpande S.R., Tupkari J.V. Cheiloscopy as an adjunct to forensic identification: a study of 600 individuals. J Forensic Odontostomatol. 2008;27(2):44-521.