Correlation of serology with morphological changes in gastric biopsy of H. pylori infection
DOI:
https://doi.org/10.18203/2320-6012.ijrms20171599Keywords:
Helicobacter pylori, Hematoxylin and eosin staining, Modified Giemsa staining, SerologyAbstract
Background: Helicobacter pylori is involved in many gastrodeudonal complications and many diagnostic tests are available for its identification. The present study was done with the objective to evaluate the morphological changes induced by H. pylori in the gastric mucosa and to correlate them with the severity of the infection.
Methods: This study was conducted in a tertiary care hospital from July 2013 to June 2014. 60 patients with symptoms of dyspepsia and requiring an upper gastrointestinal endoscopy were included in the study. Upper gastrointestinal endoscopy was performed on all patients. Hematoxylin and Eosin staining (H and E), modified Giemsa staining were performed on tissue sections and examined microscopically for gastritis and presence and absence of H. pylori.
Results: Out of 60 patients, 33 were male and 27 were females. Serology by immunochromatography technique was positive in 41 patients. Serology was found to have a sensitivity and specificity of 90.90% and 59.25% respectively. H. pylori was positive in 28 cases on H and E. With a sensitivity and specificity of 84.84% and 100% respectively. H. pylori was positive in 33 cases on modified Giemsa with a sensitivity and specificity of 100%.
Conclusions: Simultaneous morphologic and serological detection of H. pylori helps in its complete distribution and identification of its precancerous morphological nature.
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References
Marshall BJ, Warren JR. Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration. Lancet. 1984;1:1311-5.
Blaser MJ. Helicobacter pylori and the pathogenesis of gastroduodenal inflammation. J Infect Dis. 1990;161:626-33.
Wotherspoon AC, Ortiz-Hidalgo C, Falzon MR, Isaacson PG. Helicobacter pylori associated gastritis and primary B-cell lymphoma. Lancet. 1991;338:1175-6.
Graham DY, Klein PD, Evans DG, Fiedorek SC, Evans DJ Jr, Adam E, et al. Helicobcter pylori: Epidemiology,relationship to gastric cancer and the role of infants in transmission. Eur J Gatroenterol Hepatol. 1992(l);4:1-6.
Kumar V, Abul AK, Jon AC. Robbins and Cotran Pathologic basis of disease. 9th edition. New Delhi: Elsevier; 2014.
Kumar M, Yachha SK, Aggarwal R, Shukla S, Pandey R, Prasad KN, et al. Healing of chronic antral gastritis: effect of sucralfate and colloidal bismuth subcitrate. Ind J Gastroenterol. 1996;15(3):90-3.
Wu IC, Wu DC, Lu CY, Kuo CH, Su YC, Yu FJ, et al. Comparision of serum and urine ELISA methods for the diagnosis of Helicobacter pylori: a prospective pilot study. Hepatogastroenterol. 2004;51:1736-41.
Luzza F, Maletta M, Imeneo M, Marcheggiano A, Iannoni C, Biancone L, et al. Salivary specific immunoglobulins G in the diagnosis of Helicobacter pylori infection in dyspeptic patients. Am J Gastro. 1995;90(10):1820-5.
Martin-de-Argila C, Boixeda D, Canton R, Valdezate S, Mir N, De Rafeal L, et al. Usefulness of the combined IgG and IgA antibody determinations for serodiagnosis of Helicobacer pylori infection. Eur J Gastroenterol Hepatol. 1997;9(12):1191-6.
Thijs BC, van Zwet AA, Thijs WJ, Oey HB, Karrenbeld A, Stellaard F, et al. Diagnostic tests for Helicobacter pylori: a prospective evaluation of their accuracy, without selecting as the gold standard. Am J Gastroenterol. 1996;91:2125-9.
Graham DU, Evans DJ, Peacock J, Baker JT, Schrier WH. Comparison of rapid serological tests (FlexSure HP and Quickvue) with conventional ELISA for detection of H.pylori infection. Am J Gastroenterol. 1996;91:942-8.
Wilcox MH, Dent TH, Hunter JO, Gray JJ, Brown DF, Wight DG, et al. Accuracy of serology for the diagnosis of Helicobacter pylori infection; a comparison of eight kits. J Clin Pathol 1996;49:373-6.
Laine L, Lewin DN, Naritoku W, Cohen H. Prospective comparision of H and E, Giemsa and Genta stains for the diagnosis of Helicobacter pylori. Gastroint Endosc. 1997;45(6):463-7.
Genta RM, Robason GO, Graham DY. Simultaneous visualization of Helicobacter pylori and gastric morphology: a new stain. Hum Path. 1994;25:221-6.
Gill HH, Desai HG, Majumdar P, Mehta PR, Prabhu SR. Epidemiology of Helicobacter pylori: the Indian scenario. Ind J Gastroenterol. 1993;12(1):9-11.
Gill HH, Majumdar P, Shankaran K, Desai HG. Age related prevalence of Helicobacer pylori in Indian subjects. Ind J Gastroenterol. 1994;13:92-4.
Kang G, Rajan DP, Patra S, Chacko A, Mathan MM. Use of serology, the urease test and histology in diagnosis of Helicobacter pylori infection in symptomatic and asymptomatic Indians. Ind J Med Res. 1999;110:86-90.
Booth L, Holdstock G, MacBride H, Hawtin P, Gibson JR, Ireland A, et al. Clinical importance of Campylobacter pylorides and associated serum IgG and IgA antibody responses in patients undergoing upper gastrointestinal endoscopy. J Clin Pathol. 1986;39:215-9.
Perez-Perez GI, Brown WR, Cover TL, Dunn BE, Cao P, Martin JB. Correlation between serological and mucosal inflammatory responses to Helicobacter pylori. Clin Dia Lab Immun. 1994;1:325-9.
Urita Y, Hike K, Torii N, Kikuchi Y, Kurakata H, Kanda E, et al. Comparison of serum IgA and IgG antibodies for detecting Helicobacter pylori infection. Int Med. 2004;43:548-52.
She RC, Wilson AR, Litwin CM. Evaluation of Helicobacter pylori IgG, IgA and IgM serologic testing compared stool antigen testing. ClinVacc Immun. 2009;16:1253-5.
Jones DM, Lessells AM, Eldridge J. Campylobacter - like organisms on the gastric mucosa: culture, histological and serological studies. J clinpathol. 1984;37:1002-6.
Tokunaga Y, Shirahase H, Yamamoto E, Inao R, Hamaguchi S, Kanaji K, et al. Modified rapid urease test for H. pylori detection in relation to an immunohistochemical stain. J Gastroenterol Hepatol. 2000;15:617-21.
Shafii M, Nikzad SE, Kasiri H, Naghipour M. Histopathological evaluation of chronic gastritis with and without Helicobacter pylori colonization: a study from Iran. Malaysian J Pathol. 2008;30:27-30.
Malik GM, Mubarik M, Kadla SA. Helicobacter pylori infection in endoscopy biopsy specimens of gastric antrum: Laboratory diagnosis and comparative efficacy of three diagnostic tests. Diag Therp Endoscop J. 1999;6:25-9.
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