Isolation and identification of candida species from various clinical samples in a tertiary care hospital

Vignesh Kanna B., Amar Kumar G., Swapna M., Joshy M. Easow


Background: Candida spp is a member of the normal flora of the skin, mucous membrane and gastrointestinal tract. They are endogenous opportunists which cause secondary infection in individuals with underlying immunocompromised conditions. Candidiasis is a common fungal disease in humans. An increase in the prevalence of non-albicans species has been noted during the last decades because of increasing use of azoles. This study aims to Spectate Candida using chromogenic medium.

Methods: A total of 50 Candida isolates from various clinical samples were included in the study. These isolates were subjected to gram's stain, germ tube test and inoculation on commercially available CHROM agar (HiMedia India).

Results: In current study majority of isolates were from high vaginal swab (34%) followed by sputum (28%), urine (18%), pus from surgical sites and others constituted to 20%. Candida albicans (51%) was the most common candida species, followed by C. tropicalis (25%), C. krusei (16%), C. glabrata (6%) and C. dubliniensis (1%).

Conclusions: Along with Candida albicans, non-albicans candida spp like C. tropicalis, C. krusei, C. glabrata, and C. dubliniensis are increasingly being isolated from clinical samples. CHROM agar is a simple, rapid and inexpensive method for identification of such species. Characterization to species level helps to identify species which might be intrinsically resistant to commonly used antifungal agents.


Candida, CHROM agar, Non- albicans candida

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Anaissie EJ, McGinnis MR, Pfaller MA, eds. Clinical Mycology, 2nd edn. Philadelphia, Churchill Livingstone, 2009.

Mokaddas EM, Al-Sweith NA, Khan ZU. Species distribution and antifungal susceptibility of Candida bloodstream isolates in Kuwait: a 10-years study. J Med Microbiol. 2007;56:255-9.

Srinivasan L, Kenneth J. Antibiotic susceptibility of Candida isolates in a tertiary care hospital in Southern India. Ind J Med Microbiol. 2006;24:1-8.

Golia S, Reddy KM, Karjigi KS, Hittinahalli V. Speciation of Candida using chromogenic and cornmeal agar with determination of fluconazole sensitivity. Al Ameen J Med Sci. 2013;6(2):163-6.

Jain N, Mathur P, Misra MC, Behera B, Xess I, Sharma SP. Rapid identification of yeast isolates from clinical specimens in critically ill trauma ICU patients. J Lab Physicians. 2012;4(1):30-4.

Abdel AM, Taha AM, Mashal NE, Shabrawy WE. Antifungal susceptibility testing: New trends. Egyptian Dermatol Online J. 2007;3(1):1.

Lymn LH, Duane RH, Eliriton KM, Dooley D. Direct isolation of Candida spp from blood culture on the chromogenic medium CHROM agar Candida. J Clin Microbiol. 2003;41(6):2629-32.

Murray MP, Zinchuk R, Larone DH. CHROM agar Candida as the sole primary medium for isolation of yeast and as a source medium for the rapid-assimilation of trehalose test. J Clin Microbiol. 2005;43:1210-2.

Manjunath V, Vidya GS, Sharma A, Prakash MR, Murugesh N. Speciation of Candida by Hi-Chrom agar and sugar assimilation test in both HIV infected and non-infected patients. Int J Biol Med Res. 2012;3(2):1778-82.

Vijaya D, Harsha TR, Nagaratanamma T. Candida speciation using CHROM agar. J Clin Diagn Res. 2011;5(4):755-7.

Adhikary R, Joshi S. Species distribution and anti-fungal susceptibility of candidemia at a multi super-speciality center in Southern India. Ind J Med Microbiol. 2013;29:309-11.