Published: 2016-12-19

Study of iron status indicators in different phases of menstrual cycle in first year medical college females

Shipra Chandra, Narendra Gupta, Sanjay Kumar Patel


Background: Iron requirements are increased in adolescent girls with growth and the onset of menarche and remain high in women until menopause.

Methods: We conducted a study on first year medical college female students (age group 18-25 years) belonging to socioeconomic classes 1 and 2 as per the Modified Kuppuswamy’s Scale to study the effect of the phases of menstrual cycle on the iron status indicators (ISI) (Hb, Serum Iron, TIBC, MCV and TS%). Menstrual phases were defined and blood samples were drawn from all the girls in all the 3 phases (menstrual, follicular and luteal) and Hb, Serum iron and Total iron binding capacity were assessed on a semi-auto-analyzer and MCV was estimated using a fully automated CBC machine. Transferrin Saturation was derived as Serum Iron / TIBC × 100.

Results: We found significant (p<0.01) difference in iron status indicators (ISI) that varied significantly in accordance to the menstrual cycle, with values highest during the luteal phase and lowest during the menstrual phase.

Conclusions: The effect of hormonal fluctuations during the menstrual cycle plays a part in the variation of iron status measures, which has to be considered while measuring them in a female during her reproductive years.


Iron status indicators, Menstrual cycle

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WHO Geneva Switzerland. 2004:163-209.

United Nations. Second report on the world nutrition situation. Vol 1. Global and regional results. Administrative Committee on Coordination/ Subcommittee on Nutrition. 1992.

McClung JP, Karl JP, Corum SJC, Williams KW, Young AJ, Lieberman HR. Longitudinal decrements in iron status during military training in female Soldiers. J Am Coll Nutr. 2009;102(4):605-9.

Hallberg L, Rossander - Hulthénm L. Iron requirements in menstruating women. Am. J. Clin. Nutr. 1991;54:1047-58.

Kim I, Yetley EA, Calvo MS. Variations in iron-status measures during the menstrual cycle Am J Clin Nutr. 1993;58:705-9.

Harrison’s, Principles of Internal Medicine, 16th edition. Vol 1 Part 5, Section 2,Chapter 90, Pg 588.

Ghai CL. A textbook of practical physiology Jaypee Publications 7th edition. 40, 61.

Bentley ME, Griffiths PL. The burden of anemia among women in India. Euro J Clin Nut. 2003;57:52-60.

Elemo GN, Lamidi FF, Shittu SA. Iron status of premenopausal women in a Nigerian University Community Asian Jour. Of Clin. Nutr. 2010;2(3):101-7.

Gaebelein CJ, Senay LC Jr. Vascular volume dynamics during ergometer exercise at different menstrual phases. Eur. J. Appl. Physiol. 1982;50:1-11.

Reich M. The variations in urinary aldosterone levels of normal females during their menstrual cycle. Australas Ann Med. 1962;11:41-9.