Antifungal activity of various plant oils against yeast isolates from ICU patients

Akansha Suman; Sapna Chauhan; Suman Lata; Rajeev K. Sharma

doi:10.18203/2320-6012.ijrms20173018

Authors

Akansha Suman Department of Pharmacology, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, India
Sapna Chauhan Department of Microbiology, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, India
Suman Lata Department of Pharmacology, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, India
Rajeev K. Sharma Department of Pharmacology, Muzaffarnagar Medical College, Muzaffarnagar, Uttar Pradesh, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20173018

Keywords:

Antifungal activity, Essential plant oils, Resistance, Yeast isolates

Abstract

Background: To evaluate the in-vitro antifungal activity of various essential plant oils against yeast species.

Methods: The present study was a prospective study conducted in Department of Microbiology along with Department of Pharmacology at Tertiary care teaching hospital. Various clinical samples from ICU patients were inoculated on Sabourauds Dextrose Agar (SDA) in accordance with the standard methods. Yeast isolates were recovered and identified as per standard recommended procedure. The antifungal activity of plant oils against these isolated yeasts was determined using disc diffusion method. The results were interpreted as <9mm-inactive, 9-12mm-partially active, 13-18mm-very active. Fluconazole and Itraconazole were taken as control drugs.

Results: The various yeast isolated were C. albicans, C. tropicals, C. krusei, C. glabrata, C. gulliermondi, C. keyfr, Cryptococcus and Tricosporon. Among essential plant oils, Cinnamon oil and clove oil showed high activity against all isolated yeast species. Olive oil showed least antifungal activity. Fluconazole was resistant in all yeast isolates, while Itraconazole was sensitive to all yeast isolates. Sensitivity of cinnamon and clove oil was statistically significant than Itraconazole.

Conclusions: To conclude present study shows the potential of essential plant oils as newer therapeutic alternatives to antifungal drugs. These may be used in combination with antifungal agents to overcome drug resistance, adverse effects and in shortening the long-term treatment with antifungal drugs. Thus, these essential plant oils can be used in future as antifungal agents in azoles resistant strains.

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