Characterization, speciation and antimicrobial resistance pattern of Enterococcus species isolated from clinical specimens at a rural tertiary care hospital

Authors

  • Shweta Jaiswal Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India
  • Amit Singh Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India
  • Rajesh K. Verma Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India
  • Dharmendra P. Singh Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India
  • Sunita Kumari Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20173546

Keywords:

Enterococcu, E-test, Vitek®2 compact system, VRE

Abstract

Background: Enterococci have attracted much attention in recent years due to their increased recognition as a cause of nosocomial infection. They exhibit vancomycin resistance with increasing frequency so that determination of antimicrobial susceptibility pattern is necessary. Aim of present study is to determine the prevalence and susceptibility pattern of Enterococci in rural tertiary care hospital.

Methods: Total of 100 Enterococcus isolated from various clinical samples were identified and speciated by Vitek®2 compact system (BIOMERIEUX). Antibiotic susceptibility was determined by modified Kirby Bauer disc diffusion method. Results were interpreted as per CLSI guidelines and vancomycin MIC was determined by E-test method.

Results: Out of 100 Enterococcus strains 47 were E. faecalis, 51 were E. faecium, 02 were E. gallinarum and 01 was E. cassaliflavus. Resistance to penicillin, ampicillin, ciprofloxacin, elevated level gentamycin and high-level streptomycin were observed. All isolates were sensitive to linezolid and teicoplanin, 6% strains showed vancomycin resistance by E-test.

Conclusions: High rate of resistance to penicillin, ciprofloxacin and aminoglycosides is observed and emergence of VRE has further worsened this situation. So, there is an urgent need for more rational use of antimicrobials and infection control.

Author Biographies

Shweta Jaiswal, Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India

Microbiology MD (Junior resident)

Amit Singh, Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India

Microbiology (professor)

Rajesh K. Verma, Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India

Microbiology (professor)

Dharmendra P. Singh, Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India

Microbiology (Assistant professor)

Sunita Kumari, Department of Microbiology, Uttar Pradesh University of Medical Sciences, Saifai, Etawah, Uttar Pradesh, India

Microbiology(Assistant professor)

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Published

2017-07-26

How to Cite

Jaiswal, S., Singh, A., Verma, R. K., Singh, D. P., & Kumari, S. (2017). Characterization, speciation and antimicrobial resistance pattern of Enterococcus species isolated from clinical specimens at a rural tertiary care hospital. International Journal of Research in Medical Sciences, 5(8), 3484–3487. https://doi.org/10.18203/2320-6012.ijrms20173546

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Section

Original Research Articles