Comparison of BACTEC MGIT with conventional methods for detection of Mycobacteria in clinically suspected patients of extra pulmonary tuberculosis in a tertiary care hospital
DOI:
https://doi.org/10.18203/2320-6012.ijrms20173556Keywords:
Mycobacterium tuberculosis, MGIT, Lowenstein Jensen (LJ) media, Ziehl Neelsen (Z-N) stainingAbstract
Background: Tuberculosis is an important public health problem in India and globally. Extra pulmonary tuberculosis (EPTB) constitutes for approximately 15 to 20 per cent of all cases of tuberculosis in immunocompetent patients and accounts for more than 50 per cent of the cases in HIV- positive individuals. Main problem with the extra-pulmonary tuberculosis is the paucibacillary nature of the specimen, which makes the diagnosis difficult and delay the treatment. With this in background, this study aimed at the isolation of Mycobacteria from clinical specimens of patients suspected of extra pulmonary tuberculosis using BACTEC MGIT, Lowenstein Jensen (LJ) media and direct acid-fast bacilli smear examination.
Methods: A total of 66 samples were processed for direct AFB smear examination, and culture on MGIT and LJ media. Acid fast staining of the specimens was done using the Ziehl-Neelsen method.
Results: Among 66 specimens, MGIT gave a higher yield of mycobacteria (46.9%), lower contamination rate (3%) and shorter time to positive culture as compared to LJ media.
Conclusions: MGIT gives higher yield and faster results.
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References
Fanning A. Tuberculosis-extrapulmonary disease. CMAJ. 1999;160:1597-603.
World Health Organization WHO Report 2003: Global Tuberculosis Control, surveillance, Planning, Financing Geneva. Available at: http://apps.who.int/iris/bitstream/10665/63835/14/WHO_CDS_TB_2003.316.pdf.
Pfyffer GE, Welscher HM, Kissling P, Cieslak C, Casal MJ, Gutierrez J, et al. Comparison of the mycobacteria growth indicator tube (MGIT) with radiometric and solid culture for recovery of acid-fast bacilli. J Clin Microbial. 1997;35:364-8.
Sekar B, Selvaraj L, Alexis A, Ravi S, Arunagiri K, Rathinavel L. The utility of IS6110 sequence based polymerase chain reaction in comparison to conventional methods in the diagnosis of extra-pulmonary tuberculosis. Indian J Med Microbiol. 2008;26(4):352-5.
Bemer P, Palicova F, Rusch-Gerdes S, Drugeon HB, Pfyffer GE. Multicentre evaluation of fully automatic BACTEC mycobacteria growth indicator tube 960 system for susceptibility testing of Mycobacterium tuberculosis. J Clin Microbiol. 2002;40:150-4.
Tortoli E, Cichero P, Piersimoni C, Simonetti T, Gesu G, Nistta D. Use of BACTEC MGIT for recovery of mycobacteria from clinical specimens: multicentric study. J Clin Microbiol. 1999;37:3578-82.
Needham DM, Godfrey-Faussett P, Foster SD. Barriers to tuberculosis control in urban Zambia: the economic impact and burden on patients prior to diagnosis. Int J Tuberc Lung Dis. 1989;2:811-7.
Kent PT, Kubica GP. Public health mycobacteriology: a guide for the level III laboratory. US Department of health and human services, public health service, centers for disease control; 1985.
Kaur J, Singh J, Mishra P. Comparative evaluation of CFX96TM real time PCR with conventional PCR for rapid diagnosis of Mycobacterium tuberculosis complex in clinical isolates. MedPub J. 2016;7(4):24.
Hanna BA, Ebrahimzadeh A, Elliott LB, Morgan MA, Novak SM, Rusch-Gerdes S, et al. Multicentre evaluation of the BACTEC MGIT 960 system for recovery of mycobacteria. J Clin Microbiol. 1999;37:748-52.
Dongsi L, Dunnc M. Comparison of the automated mycobacteria growth indicator tube system (BACTEC MGIT 960) with Lowenstein Jensen medium for recovery of mycobacteria from clinical specimens. Microbiol Infect Dis. 2002;118:542-5.
Hines N, Payeur JB, Hoffman LJ. Comparison of the recovery of Mycobacterium bovis isolates using the BACTEC 460 TB system and middle brook 7H10 and 7H11 solid media. J Vet Diagn Invest. 2006;18:243-50.
Rishi S, Malhotra B, Pal N. A Comparative study for the detection of mycobacteria by BACTEC MGIT 960, Lowenstein Jensen media and direct AFB smear examination. Ind J Med Microbiol. 2007;25(4).
Bunger R, Singh VA, Avneet, Mehta S, Pathania D. Evaluation of BACTEC Micro MGIT with Lowenstein Jensen media for detection of Mycobacteria in clinically suspected patients of extra pulmonary tuberculosis in a tertiary care hospital at Mullana (Ambala). J Med Microb Diagn. 2013,2:2.
Chihota VN, Grant AD, Fielding K, Ndibongo B, Zyl AV, Muirhead D, et al. Liquid versus solid culture for tuberculosis: performance and cost in a resource-constrained setting. Int J Tuberc Lung Dis. 2010;14(8):1024-31.
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