Quantification of serum hepcidin as a potential biomarker in diagnosis of iron deficiency anaemia

Authors

  • Sonal Vyas Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India
  • Anil Kapoor Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India
  • S. K. Nema Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India
  • Sanjeev Suman Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India

DOI:

https://doi.org/10.18203/2320-6012.ijrms20172546

Keywords:

Anemia of chronic disease, Iron deficiency anemia, Serum hepcidin, Serum ferritin, IL-6

Abstract

Background: Iron deficiency (ID) is the most prevalent nutritional deficiency and the most common cause of anemia in the world. Present study aims to detect the serum hepcidin and ferritin levels in iron deficiency induced anaemia’s and compare and correlate the values of serum hepcidin levels with their Serum ferritin levels and IL-6 levels.

Methods: A total of 94 individuals were enrolled in the study. Sample for hematological evaluations were collected and estimation was carried out for biomarker estimation by ELISA method(s) using specified kit(s) procured commercially. The statistical evaluation was done using SPSS version 24.0. Analysis of variance (ANOVA) and Pearson's correlation tests were used to compare the variables and to see the correlation between the different variables.

Results: Serum Hepcidin, a marker of iron deficiency anemia is significantly low in patients with IDA (33.23±12.46 ng/mL) than in normal with p-value <0.001 which is highly significant. In present study, we determined the cut off points differentiating IDA from healthy group which was ≤ 34.55; with AUC 0.845 (P<0.0001), 95% confidence interval was 0.755 to 0.911, and sensitivity was 98.33 % and specificity 52.94%. These cut off points had strong confidence interval and valuable predictive potential.

Conclusions: Serum Hepcidin can be used as a simple and cost effective diagnostic marker for identification of anemia.

Author Biographies

Sonal Vyas, Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India

Index Medical College, Hospital & Research Center, Indore, M.P, India.

Anil Kapoor, Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India

Index Medical College, Hospital & Research Center, Indore, M.P, India.

S. K. Nema, Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India

Index Medical College, Hospital & Research Center, Indore, M.P, India.

Sanjeev Suman, Department of Pathology, Index Medical College, Hospital and Research Centre, Indore, Madhya Pradesh, India

Index Medical College, Hospital & Research Center, Indore, M.P, India.

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Published

2017-06-24

How to Cite

Vyas, S., Kapoor, A., Nema, S. K., & Suman, S. (2017). Quantification of serum hepcidin as a potential biomarker in diagnosis of iron deficiency anaemia. International Journal of Research in Medical Sciences, 5(7), 2926–2930. https://doi.org/10.18203/2320-6012.ijrms20172546

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Section

Original Research Articles