DOI: http://dx.doi.org/10.18203/2320-6012.ijrms20173582

Screening of Elaeocarpus floribundus fruit extracts for bioactive phytocomponents and antibacterial activity against food-borne bacteria

Bijayanta Sircar, Shyamapada Mandal

Abstract


Background: Medicinal plants possess several active components having antimicrobial activity. This study was undertaken to explore the antibacterial activity of Indian olive, Elaeocarpus floribundus, fruit extracts against potential food-borne bacterial isolates.

Methods: The ethanolic extracts of olive seed (OSE) and mesocarp-epicarp (OMeE), and the aqueous extracts of olive seed (AqOSE) and mesocarp-epicarp (AqOMeE) were prepared, and analysed qualitatively for phytochemicals. The antibacterial activity of the extracts against food-borne pathogenic bacteria: Bacillus sp., Bacillus cereus, Enterococcus sp. and Corynebacterium sp., was determined by agar-well diffusion method, and minimum inhibitory concentration (MIC) values by agar dilution method.

Results: The concentration dependent activity of the extracts against the bacteria was recorded with zone diameter of inhibition 6 - 28 mm for ethanolic extracts, and 7 - 23 mm for aqueous extracts. The ethanolic extracts were confirmed positive for the presence of cardiac glycosides, anthraquinone glycosides, steroids, terpenoids and quinones, while cardiac glycosides, anthraquinone glycosides, steroids, terpenoids, quinones and phenol were detected in the aqueous extracts. The MICs of OSE and OMeE ranged 9.375-12.5mg/ml, and 1.875 - 3.125 mg/ml, respectively, for the test bacteria.

Conclusions: The olive fruit extracts contained various bioactive components, and had excellent antibacterial activity against food-borne bacteria. The plant might be useful in the preparation of non-antibiotic antibacterial agents and in the storage of food as well.


Keywords


Antibacterial activity, Food-borne bacterial pathogens, Minimum inhibitory concentration, Olive fruit, Phytochemicals

Full Text:

PDF

References


Mandal S, Mandal MD, Pal NK. Antibiotic resistance of Salmonella enterica Serovar Typhi in Kolkata, India, and in vitro experiments on effects of combined chemotherapy. Scientific World J. 2012; ID454059:1-4.

Yadav M, Pal R, Damrolien S, Khumanthem SD. Microbial spectrum of urinary tract infections and its antibiogram in a tertiary care hospital. Int J Res Med Sci. 2017;5(6):2718-22.

Amir F, Wong KC, Eldeen I, Asmawi MZ, Osman H. Evaluation of biological activities of extracts and chemical constituents of Mimusops elengi. Trop J Pharm Res. 2013;12(4):591-6.

Nair R, Kalariya T, Chanda S. Antibacterial activity of some selected Indian medicinal flora. Turkish J Biol. 2005;29(1):41-7.

Chadha ML. Indigenous Vegetables of India with a Potential for Improving Livelihoods. International Symposium on Underutilized Plants for Food Security, Nutrition, Income and Sustainable Development. 2009; 2:1-8.

Shah BN, Seth AK. Pharmacological potential of Trichosanthes dioica – an edible plant. Hygeia J D Med. 2010;2(2):1-7.

Zaman S. Exploring the antibacterial and antioxidant activities of Elaeocarpus floribundus leaves. IAJPS. 2016;3(2):92-7.

Aguilera CM, Mesa MD, Ramirez-Tortosa MC, Nestares MT, Ros E, Gil A. Sunflower oil does not protect against LDL oxidation as virgin olive oil does in patients with peripheral vascular disease. Clin Nutr. 2004;23(4):673-81.

Bulotta S, Celano M, Lepore SM, Montalcini T, Pujia A, Russo D. Beneficial effects of the olive oil phenolic components oleuropein and hydroxytyrosol: focus on protection against cardiovascular and metabolic diseases. J Transl Med. 2014;12:219.

Chowdhury KA, Ghosh SS. Indian woods, their identification, properties and uses. Delhi, Vol. I: Dilleniaceae to Elaeocarpaceae, India: Manager of Publications. 1958.

Yusuf M, Begum J, Hoque MN, Chowdhury JU. Medicinal plants of Bangladesh. 2nd ed. BCSIR Laboratories, Chittagong. 2009:599.

Ghani A. Medicinal Plants of Bangladesh. Asiatic Society of Bangladesh, Dhaka, Bangladesh. 2nd ed. 2003.

Markin D, Duek L, Berdicevsky I. In vitro antimicrobial activity of olive leaves. Mycoses. 2003;46(3-4):132-6.

Khalil MMH, Ismail EM, El-Baghdady KZ, Mohamed D. Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arabian J Chem. 2014;7:1131-9.

Wiart C. Medicinal plants of the Asia-Pacific: Drugs for the future? World Scientific. 2006:87-9.

Kubo I, Matsumoto A, Takase I. A multi chemical defense mechanism of bitter olive Olea europaea (Oleaceae). Is oleuropein a phytoalexin precursor? J Chem Ecol. 1985;47(11):251-63.

Teuber M. Spread of antibiotic resistance with food-borne pathogens. Cell Mol Life Sci. 1999:56:755.

Mandal S, Mandal MD, Pal NK. Synergism Between Mimusops elengi and Bauhinia variegata Seed Extracts Against Salmonella enteric serovar Typhi and Vibrio cholerae O1 Biotype El Tor Serotype Ogawa Isolates. Int J Sci Nat. 2014;5(2):191-5.

Radhakrishnan K, Thangamani P, Balakrishnan V. Antibacterial and phytochemical analysis of stem and root extracts of Calotropis gigantea against selected pathogens. Malaya J Biosci. 2014;1(1):49-55.

Ayoola GA, Coker HB, Adesegun SA, Adepoju-Bello AA, Obaweya K, Ezennia EC et al. Phytochemical screening and antioxidant activities of some selected medicinal plants used for malaria therapy in southwestern Nigeria. Trop J Pharm Res. 2008;7(3):1019-24.

Joshi A, Bhobe M, Saatarkar A. Phytochemical investigation of the roots of Grewiamicrocos Linn. J Chem Pharm. Res. 2013;5(7):80-7.

Naik LS, Shyam P, Marx KP, Baskari S, Devi CVR. Antimicrobial Activity and Phytochemical Analysis of Ocimum tenuiflorum Leaf Extract. Int J Pharm Tech Res. 2015;8(1):88-95.

Clinical and Laboratory Standards Institute (CLSI): Performance standards for antimicrobial susceptibility testing 21st informational supplement M100S21. CLSI, Wayne, Pa;2011.

Mandal S, Mandal MD, Pal NK. In vitro efficacy of ciprofloxacin alone and in combination with amoxicillin against Salmonella typhi isolates. Indian J Exp Biol. 2003;41:360-2.

Munide OK, Kuria E. Hygienic and Sanitary practices of vendors of street foods in Nairobi Kenya. Afr J food Agric Nutr Dev. 2005;5(1):1-13.

De Boer E, Dod R, Beuner. Methodology for Detection of food- borne Microorganisms. Journal of food protection. 2001;66:1587- 1589.

Medina E, Romero C, Brenes M, de Castro A. Antimicrobial Activity of Olive Oil, Vinegar, and Various Beverages against Foodborne Pathogens. J Food Prot. 2007;70(5):1194-9.

Karaosmanoglu H, Soyer F, Ozen B, Tokatli F. Antimicrobial and Antioxidant Activities of Turkish Extra Virgin Olive Oils. J Agric Food Chem. 2010;58:8238-45.

Sousa A, Ferreira ICFR, Calhelha R, Andrade PB, Valentão P, Seabra R et al. Phenolics and antimicrobial activity of traditional stoned table olives ‘alcaparra’. Bioorg Med Chem. 2006;58(14):8533-8.

Korukluoglu M, Sahan Y, Yigit A, Ozer ET, Gücer S. Antibacterial activity and chemical constitutions of Olea europaea l. Leaf extracts. J Food Process. Preserv. 2010;34:383-96.

Sharvani KA, Jagadeesh D, Chandrakanth R, Sumana K, Seema M, Devaki NS. Antimicrobial assay of Elaeocarpus species of western ghats of Karnataka. Asian J Pharm Anal Med Chem. 2015;3(1):14-19.

Sharma A, Joshi S, Kumar N. Antioxidant and antibacterial properties of leaves of Elaeocarpus sphaericus Roxb. and Pinus wallichiana from Uttarakhand region of India. ‎Int J Green Pharm. 2015;9(4):246-51.

Shazid MD, Sharker, Shahid IJ. Assessment of antibacterial and cytotoxic activity of some locally used medicinal plants in Sundarban mangrove forest region. Afr J Pharm Pharmacol. 2010;4(2):066-9.

Sircar B, Mandal S. Antibacterial activity of Mimusops elengi leaf, seed and bark extracts alone and in combination with antibiotics against human pathogenic bacteria. Transl Med (Sunnyvale). 2016;6(4):188.

Mandal S, Mandal MD, Pal NK. Antibacterial potential of Azadirachta indica seed and Bacopa monniera leaf extracts against multidrug resistant Salmonella enterica serovar Typhi isolates. Arch Med Sci. 2007;3(1):14-8.

Sudjana AN, D’Orazio C, Ryan V, Rasool N, Ng J, Islam N et al. Antimicrobial activity of commercial Olea europaea (olive) leaf extract. Int J Antimicrob Agents. 2009;33(5):461-3.

Pereira JA, Pereira APG, Ferreira ICFR, Valentão P, Andrade PB, Seabra R et al. Table olives from Portugal: phenolic compounds, antioxidant potential and antimicrobial activity. J Agric Food Chem. 2006;54(22):8425-31.

Proestos C, Chorianopoulos N, Nychas GJE, Komaitis M. RP-HPLC analysis of the phenolic compounds of plant extracts. Investigation of their antioxidant capacity and antimicrobial activity. J Agric Food Chem. 2005;53(4):1190-5.

Rauha JP, Remes S, Heinonen M, Hopia A, Kähkönen M, Kujala T et al. Antimicrobial effects of Finnish plant extracts containing flavonoids and other phenolic compounds. Int J Food Microbiol. 2000;56(1):3-12.

Zhu X, Zhang H, Lo R. Phenolic compounds from the leaf extract of artichoke (Cynara scolymus L.) and their antimicrobial activities. J Agric Food Chem. 2004;52(24):7272-8.

Puupponen-Pimiä R, Nohynek L, Meier C, Kähkönen M, Heinonen M, Hopia A et al. Antimicrobial properties of phenolic compounds from berries. J Appl Microbiol. 2001;90(4):494-507.

Medina E, Castro AD, Romero C, Brenes M. Comparison of the concentrations of phenolic compounds in olive oils and other plant oils: correlation with antimicrobial activity. J Agric Food Chem. 2006;54(14):4954-61.